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Effect of catechins and citrus flavonoids on invasionin vitro

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Abstract

Catechins, a group of flavonoid molecules, inhibit invasion of mouse MO4 cells into embryonic chick heart fragmentsin vitro. The anti-invasive effects can be ranked as follows: (+)-catechin > (−)-epicatechin>3-O-methyl-(+)-catechin > 3-O-palmitoyl-(+)-catechin. Most of the catechins are unstable in cell culture media, and their spontaneous rearrangement products tend to bind to extracellular matrix (ECM). Due to these interactions proteases such as tissue-type plasminogen activator (t-PA) are linked to the ECM glycoprotein laminin. This leads to a partial inactivation of the enzyme. Within the group of catechins we found a positive correlation between anti-invasive activity and linking of t-PA to laminin. Citrus flavonoids are also anti-invasivein vitro (tangeretin > nobiletin > hesperidin = naringin). However, these stable molecules show poor affinity for ECM, and do not link enzymes to laminin. These data suggest that catechins and citrus flavonoids inhibit invasionin vitro by different mechanisms.

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Authors and Affiliations

  1. Laboratory of Experimental Cancerology, Department of Radiotherapy and Nuclear Medicine, University Hospital, Gent, Belgium

    M. Bracke, B. Vyncke & M. Mareel

  2. Rega Institute for Medical Research, University of Leuven, Belgium

    G. Opdenakker

  3. Laboratory of Dermatology and Department of Gynecology and Obstetrics, University of Liège, Belgium

    J. -M. Foidart

  4. Laboratory of Embryology, University of Gent, Belgium

    G. De Pestel

Authors
  1. M. Bracke
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  2. B. Vyncke
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  3. G. Opdenakker
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  4. J. -M. Foidart
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  5. G. De Pestel
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  6. M. Mareel
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Bracke, M., Vyncke, B., Opdenakker, G. et al. Effect of catechins and citrus flavonoids on invasionin vitro . Clin Exp Metast 9, 13–25 (1991). https://doi.org/10.1007/BF01831706

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  • DOI: https://doi.org/10.1007/BF01831706

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