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Basement membrane invasion by first trimester human trophoblast: requirement for branched complex-type Asn-linked oligosaccharides

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Abstract

Trophoblast cells of normal first trimester human placenta share with malignant tumor cells the ability for significant cellular proliferation and invasion of basement membranes. Because tumor cell metastasisin vivo and invasion of basement membranesin vitro have recently been shown to require the expression of-GIcNAcβ1–6 Manαl-6 Manβl-branched complex type Asn-linked oligosaccharides in tumor cell surface glycoproteins, we decided to determine if such structures were also necessary for invasion by trophoblast cells. We report here that invasive first trimester trophoblasts express leukoagglutinin-reactive βl-6 branched Asn-linked oligosaccharides on their surface. Moreover, basement membrane invasion by trophoblast was significantly inhibited by pretreating the cells with swainsonine, a non-toxic inhibitor of Golgi α-mannosidase II which blocks β1–6 branching of Asn-linked oligosaccharides. The first trimester trophoblast cells pretreated with swainsonine attached more avidly to the amnion basement membrane and to an extracellular matrix (ECM) preparation compared to control non-treated erophoblast cells. Swainsonine treatment did not inhibit secretion of gelatinase or plasminogen activator activities by trophoblast cells. These results suggest that expression of βl-6 branched oligosaccharides in trophoblast cells may be functionally important for the implantation and placentation processes by reducing cell adhesion to ECM and thereby facilitating trophoblast cell invasion.

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Abbreviations

L-PHA:

leukoagglutinin

ECM:

extracellular matrix

Man:

mannose

Gal:

galactose

G1cNAc:

N-acetylglucosamine

SA:

sialic acid

βl-6 branch:

-GlcNAcβ1–6Manα1–6Manβ1- linked antenna of complex-type Asn-linked oligosaccharides

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Authors and Affiliations

  1. Division of Cancer and Cell Biology, Mount Sinai Hospital Research Institute, 600 University Avenue, M5G 1X5, Toronto, Ontario

    S. Yagel, R. Kerbel & J. W. Dennis

  2. Department of Medical Genetics, University of Toronto, Canada

    S. Yagel, R. Kerbel & J. W. Dennis

  3. The Department of Obstetrics and Gynecology, Hadassah University Hospital, Jerusalem, Israel

    S. Yagel & T. Eldar-Gera

  4. Department of Anatomy, The University of Western Ontario, N6A 5C1, London, Ontario, Canada

    S. Yagel & P. Lala

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  1. S. Yagel
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  2. R. Kerbel
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  4. T. Eldar-Gera
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  5. J. W. Dennis
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Yagel, S., Kerbel, R., Lala, P. et al. Basement membrane invasion by first trimester human trophoblast: requirement for branched complex-type Asn-linked oligosaccharides. Clin Exp Metast 8, 305–317 (1990). https://doi.org/10.1007/BF01810677

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  • DOI: https://doi.org/10.1007/BF01810677

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