Summary
Malignant cystosarcoma phylloides (CP) is a relatively rare cancer of the breast. A CP tumor was processed as part of a tumor acquisition, propagation, and preservation program in patient biotherapy. Two tissue culture cell lines were developed from this tumor, one directly from the biopsy, another from a xenograft tumor grown in athymic mice. The two cell lines were similar in character. There was strong immunochemical reactivity with antibodies to vimentin, type I collagen, and type III collagen. There was no reactivity with antibodies to cytokeratin and epithelial membrane antigen. Both cell lines were aneuploid, clonogenic in soft agar, and tumorigenic in nude mice. 5α-dihydrotestosterone and thyroxine added to the culture medium stimulated growth, while testosterone, 17β-estradiol, and 4-hydroxytamoxifen were without effect. Dexamethasone and cortisol were inhibitory at high doses (10−6M). Dibutyryl cyclic AMP, theophylline, and vitamin C were all inhibitory. The biopsy contained tumor-infiltrating lymphocytes which proliferated in cultures containing interleukin 2. The expanded lymphocytes were activated T cells which had the capacity to lyse tumor cells. These results suggest possibilities in the therapy of cystosarcoma phylloides involving vitamin C, certain hormones, and tumor-infiltrating lymphocytes.
References
Lee BJ, Pack CT: Giant intracanalicular fibrodenomyxoma of the breast. The so-called cytosarcoma phylloides mammae of Johannes Muller. Ann Surg 93: 250–268, 1931
Halverson JD, Hori-Rubaina JM: Cystosarcoma phylloides of the breast. American Surg 40: 295–301, 1974
Norris HJ, Taylor HB: Relationship of histologic features to behavior of cystosarcoma phylloides. Cancer 20: 2090–2099, 1967
Toher C: Cystosarcoma phylloides, an ultrastructural study. Cancer 21: 1171–1179, 1968
Rao BR, Meyer JS: Progesterone receptor in cystosarcoma phylloides. Arch Surg 112: 620–622, 1977
Palshof T, Blichert-Toft M, Daehnfeldt JL: Estradiol binding protein in cystosarcoma phylloides of the breast. Eur J Cancer 16: 591–593, 1980
Kesterson GHD, Georgeade N, Seigler HF, Barton TK, McCarty KS, McCarty KS Jr: Cystosarcoma phylloides: A steroid receptor and ultrastructure analysis. Ann Surg 190: 640–647, 1979
Tokuda Y, Kubota M, Ueyama Y, Maruo K, Hata J-I, Tamaoki N, Tajima T, Mitomi T: A malignant cystosarcoma with positive estrogen receptor and its heterotransplantation into nude mice. Cancer 55: 370–374, 1985
Lewko WM, Vaghmar RV, Hubbard D, Moore M, He YJ, Chang L, Husseini S, Wallwork K, Thurman GB, Oldham RK: Cultured cell lines from human breast cancer biopsies and xenografts. Breast Cancer Res Treat 17: 121–129
Lasfargues EY, Ozzello L: Culture of human breast carcinomas. J Natl Cancer Inst 21: 1131–1148, 1958
Horwitz KB, Koseki Y, McGuire WL: Estrogen control of progesterone receptors in human breast cancer: role of estradiol and anti-estrogen. Endocrinology 103: 1742–1752, 1978
Liao S-K, Meranda C, Avner BP, Romano T, Husseini S, Kimbro B, Oldham RK: Immunohistochemical phenotyping of human solid tumors with monoclonal antibodies in devising biotherapeutic strategies. Cancer Immunol Immunother 28: 71–86, 1989
Krishan A: Rapid flow cytometric analysis of mammalian cell cycle by propidium iodide staining. J Cell Biol 66: 188–193, 1975
Ali-Osman F, Beltz PA: Optimization and characterization of the capillary human tumor clonogenic cell assay. Cancer Res 48: 715–724, 1988
Maleckar JR, Friddell CS, Sferruzza A, Thurman GB, Lewko WM, West HW, Oldham RK, Yannelli JR: Activation and expansion of tumor derived activated cells (TDAC). J Natl Cancer Inst 1: 1655–1660, 1989
Lewko WM, Ladd P, Hubbard D, He Y-J, Vaghmar R, Husseini S, Chang L, Moore M, Thurman GB, Oldham RK: Tumor acquisition, propagation and preservation, the culture of human colorectal cancer. Cancer 64: 1600–1608, 1989
Bindal RD, Katzenellenbogen JA: Bis (4-hydroxyphenyl) [α- (phenoxysulfonyl) phenyl] methane: isolation and structure elucidation of a novel estrogen from commercial preparations of phenol red (phenosulfonphthalein). J Med Chem 31: 1978–1983, 1988
Cooperative Breast Cancer Group: Androgens and estrogens in treatment of disseminated mammary carcinoma: retrospective study of 944 patients. JAMA 172: 1271–1275, 1960
Kassem NY, Neri RO, Munroe JS: Effect of Flutamide, an anti androgen, on stage D cancer of the prostate. Clin Pharmacol Ther 29: 256–262, 1981
Vonderhaar BK, Greco AE: Lobulo-alveolar development of mouse mammary glands is regulated by thyroid hormones. Endocrinology 104: 409–418, 1979
Vonderhaar BK, Greco AE: Effect of thyroid status on development of spontaneous mammary tumors in primiparous C3H mice. Cancer Res 42: 4553–4561, 1982
Kapdi CC, Wolfe JN: Breast cancer. Relationship to thyroid supplements for hypothyroidism. JAMA 236: 1124–1127, 1976
Froehlich JE, Rochmeler M: Effect of adenosine 3′:5′-cyclic monophosphate on cell proliferation. J Cell Biol 55: 19–31, 1972
Frimes WJ, Schroeder JL: Dibutyryl cyclic adenosine 3′:5′-monophosphate, sugar transport and regulatory control of cell division in normal and transformed cells. J Cell Biol 56: 487–491, 1973
Rosenberg AM, Packard BS, Aebersold PM: Use of tumorinfiltrating lymphocytes and interleukin-2 in the immunotherapy of patients with metastatic melanoma. New Engl J Med 319: 1676–1679, 1988
Bush DA, Winkler MA: Isoelectricfocusing at cross-linked monoclonal antibody heterodimer, homodimers and derivatized monoclonal antibodies. J Chromatog 489: 303–311, 1989
Hayashi H, Kiyota T, Sakamoto H, Seto M: An enzyme linked immunosorbent assay for recombinant human tumor necrosis factor using monoclonal antibody. In: Ishigami J (ed), Recent Advances in Chemotherapy. University of Tokyo Press, 1985, pp 820–822.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lewko, W.M., Vaghmar, R., Maleckar, J.R. et al. Cultured breast cystosarcoma phylloides cells and applications to patient therapy. Breast Cancer Res Tr 17, 131–138 (1990). https://doi.org/10.1007/BF01806293
Issue Date:
DOI: https://doi.org/10.1007/BF01806293