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Changes in adhesive properties of tumor cells do not necessarily influence metastasizing capacity

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Abstract

BSp6S and BSp73AS are two rat tumors which grow locally after intra-footpad (ifp) application. BSp73ASML and BSp6AS are variants, which metastasize via the lymphatics. Both variants have lost adherence properties, as shown byin vitro culture on plastic surfaces, suggesting that loss of adherence may be accompanied by increased metastasizing capacity. However, after growth of BSp6S and BSp73ASin vitro on poly(2-hydroxyethylmethacrylate) (polyHEMA)-coated plates, which resulted in loss of adherence and spreading, and subsequent intravenous (iv) or ifp injection of non-adherent tumor cells into syngeneic rats, metastasizing capacity was not increased. It is concluded that loss of adherence may facilitate metastatic spread, but certainly is not sufficient for initiation.

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Abbreviations

BSS:

Hank's balanced salt solution

RPMI-s:

RPMI 1640, supplemented with antibiotics,l-glutamine and 10% fetal calf serum

LN:

lymph nodes

SC:

spleen cells

Mo:

peritoneal macrophages

iv:

intravenous

ifp:

intra-footpad

sc:

subcutaneous

ip:

intraperitoneal

PH/polyHEMA:

poly(2-hydroxyethylmethacrylate)

IFN:

interferon-gamma

CP:

Corynebacterium parvum

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Authors and Affiliations

  1. Institute of Radiology and Pathophysiology, German Cancer Research Center, D-6900, Heidelberg 1, F.R.G.

    M. Zöller & S. Matzku

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  1. M. Zöller
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  2. S. Matzku
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Zöller, M., Matzku, S. Changes in adhesive properties of tumor cells do not necessarily influence metastasizing capacity. Clin Exp Metast 7, 227–242 (1989). https://doi.org/10.1007/BF01787026

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  • DOI: https://doi.org/10.1007/BF01787026

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