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Multiple forms of soluble monophenol, dihydroxyphenylalanine: oxygen-oxidoreduetase (EC 1.14.1$.1) from potato tubers (Solanum tuberosum)

Multiple Formen löslicher Monophenol, Dihydroxyphenylalanin: O2-Oxidoreductase (EC 1.14.18.1) aus Kartoffeln (Solanum tuberosum)

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Zusammenfassung

Mit Hilfe der Polyacrylamidgel-Elektrophorese wurden in einer löslichen Phenoloxidase aus Kartoffeln (SorteMaritta) 17 multiple Formen mit Aktivität gegenüber Dopa und einer Reihe anderer o-Diphenole nachgewiesen, aber nur 5 dieser Formen reagierten mit Monophenolen. Durch isoelektrisches Fokussieren des Rohenzyms wurden 2 Hauptpeaks mit Aktivität gegenüber Dopa erhalten, deren isoelektrischen Punkte in den pH-Bereichen 4,0--4,7 und 5,1-5,4 lagen; daneben wurden auch kleinere Mengen Enzym bei höheren pH-Werten nachgewiesen. Bei der elektrophoretischen Reinheitsprüfung der Aktivitätspeaks wurden alle Banden, die vorher durch Polyacrylamidgel-Elektrophorese des Rohenzyms erhalten wurden, wiederaufgefunden, aber alle Peaks waren elektrophoretisch heterogen.

Durch Gelchromatographie wurde das Rohenzym in mehrere Formen unterschiedlichen Molekulargewichtes aufgetrennt, die der monomeren, dimeren, tetrameren, oktameren und einer polymeren (mindestens hexadekameren) Form des Enzyms entsprachen. Das Molekulargewicht der monomeren Form lag bei etwa 36000.

Summary

Upon polyacrylamide gel electrophoresis, a soluble phenoloxidase from potatoes (var. Maritta) revealed 17 multiple forms with activity towards dopa and almost all othero-diphenols tested, but only 5 of the forms reacted with monophenols. Isoelectric focusing of the crude enzyme resulted in 2 main peaks with activity towards dopa, having isoelectric points at pH ranges 4.0–4.7 and 5.1–5.4; smaller amounts of the enzyme at higher pI values were also detected. When activity peaks were controlled by polyacrylamide gel electrophoresis, all bands previously detected by electrophoresis of the crude enzyme were recovered, but all peaks were electrophoretically heterogeneous.

Gel chromatography of the crude enzyme showed different molecular forms. Their molecular weights indicated monomer, dimer, tetramer, octamer and polymer (at least hexadecamer) forms with a monomer molecular weight of about 36000.

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References

  1. Comprehensive Biochemistry (Florkin,M., Stotz,E.H., eds.), Vol. 13, 3rd ed., p. 115. Amsterdam, London, New York: Elsevier Scientific Publishing 1973

  2. Goddard, D.R., Holden, C.: Arch. Biochem.27. 41 (1958)

    Google Scholar 

  3. Ugazio, G.: Boll. Soc. Ital. Biol. Sper.36, 366 (1960)

    PubMed  Google Scholar 

  4. Alberghina, F. A. M.: Phytochemistry3, 65 (1964)

    Google Scholar 

  5. Craft, C. C.: Am. Potato J.43, 112 (1966)

    Google Scholar 

  6. Abukharma, D.A., Woolhouse, H.W.: New Phytologist65, 477 (1966)

    Google Scholar 

  7. Constantinides, S.M., Bedford, C.L.: J. Food Sci.32, 446 (1967)

    Google Scholar 

  8. Vincent, D., Perrier, H.: Compt. Rend. Soc. Biol.162, 2153 (1968)

    Google Scholar 

  9. Vecher, A. S., Vasil'kevich, 0. K., Reshetnikov, V. N.: Plastidnyi App. Zhiznedeyatel. Rast.1971, 81

  10. Ruis, H.: Phytochemistry11, 53 (1972)

    Google Scholar 

  11. Patil, S. S., Evans, H. T., McMahill, P.: Nature200, 1322 (1963)

    PubMed  Google Scholar 

  12. Patil, S.S., Zucker, M.: J. Biol. Chem.240, 3938 (1965)

    PubMed  Google Scholar 

  13. Vecher, A. S., Reshetnikov, V. N., Vasil'kevich, O. K.: Dokl. Akad. Nauk Belorussk. SSR11, 257 (1967)

    Google Scholar 

  14. Balasingam, K., Ferdinand, W.: Biochem. J.118, 15 (1970)

    PubMed  Google Scholar 

  15. Cheung, K.W.-K., Henderson, H.M.: Phytochemistry11, 1255 (1972)

    Google Scholar 

  16. Vesterberg, O.: In: Methods in Enzymology (Colowick, S. P., Kaplan, N. O., Jakoby, W. B., eds.), Vol. 22, p. 389. New York, London: Academic Press 1971

    Google Scholar 

  17. Davis, B.J.: Ann. N. Y. Acad. Sei.121, 404 (1964)

    Google Scholar 

  18. Maurer, H. R.: Disc Electrophoresis, p. 44. Berlin, New York: Walter de Gruyter 1971

    Google Scholar 

  19. Ashida, M.: Arch. Biochem. Biophys.144, 749, (1971)

    PubMed  Google Scholar 

  20. Chet, I., Retig, N., Henis, Y.: J. Gen. Microbiol.72, 451 (1972)

    Google Scholar 

  21. Kubowitz, F.: Biochem. Z.292, 221 (1937)

    Google Scholar 

  22. Bluhme, H., Frohne, D.: Planta Med.24, 219 (1973)

    PubMed  Google Scholar 

  23. Coggon, P., Moss, A. G., Sanderson, G.W.: Phytochemistry12, 1947 (1973)

    Google Scholar 

  24. Kertsz, D., Zito, R.: Biochim. Biophys. Acta96, 447 (1965)

    Google Scholar 

  25. Jolley, R.L., Robb, D. A., Mason, H. S.: J. Biol. Chem.244, 1593 (1969)

    PubMed  Google Scholar 

  26. Nakamura, T., Sho, S., Ogura, Y.: J. Biochem. (Tokyo)59, 481 (1966)

    Google Scholar 

  27. Hare, E., Mayer, A. M.:Phytochemistry7, 199 (1968)

    Google Scholar 

  28. Fox, A.S., Burnett, J.B.: Biochim. Biophys. Acta61, 108 (1962)

    PubMed  Google Scholar 

  29. Fling, M., Horowitz, N.H., Heinemann, S. F.: J. Biol. Chem.238, 2045 (1963)

    PubMed  Google Scholar 

  30. Harel, E., Mayer, A. M., Lehmann, E.: Phytochemistry12, 2649 (1973)

    Google Scholar 

  31. Bouchilloux, S., McMahill, P., Mason, H.S.: J. Biol. Chem.238, 1699 (1963)

    PubMed  Google Scholar 

  32. Duckworth, H.W., Coleman, J. E.: J. Biol. Chem.245, 1613 (1970)

    PubMed  Google Scholar 

  33. Dizik, N. S., Knapp, F. W.: J. Food Sci.35, 282 (1970)

    Google Scholar 

  34. Edwards, R. L., Elsworthy, G. C.: Chem. Comm.1967, 373

  35. Steglich, W., Furtner, W., Prox, A.: Z. Naturforsch.23b, 1044 (1968)

    Google Scholar 

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  1. Institut für Lebensmittelchemie der TU München, Lothstr. 17, D-8000, München 2, Germany

    Günter Mathefis & Hans-Dieter Belitz

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  1. Günter Mathefis
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  2. Hans-Dieter Belitz
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Dedicated to Prof. Dr. Dr. W. Diemair on his 75th birthday.

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Mathefis, G., Belitz, HD. Multiple forms of soluble monophenol, dihydroxyphenylalanine: oxygen-oxidoreduetase (EC 1.14.1$.1) from potato tubers (Solanum tuberosum). Z Lebensm Unters Forch 157, 221–227 (1975). https://doi.org/10.1007/BF01785774

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  • DOI: https://doi.org/10.1007/BF01785774

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