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Purification of myxamoebal fragmin, and switching of myxamoebal fragmin to plasmodial fragmin during differentiation ofPhysarum polycephalum

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Summary

We have isolated and purified an activity from amoebae ofPhysarum polycephalum that reduces the flow birefringence of a solution of F-actin in a Ca2+-dependent manner. The purified activity from 100 g of amoebae consisted of 1 mg of a 40000 mol. wt protein. DNase I-affinity chromatography demonstrated that the protein binds toPhysarum actin in a Ca2+-dependent manner, and the binding is not reversed by excess EGTA. Viscometric measurement indicated that the protein (i) accelerates polymerization of G-actin, and (ii) severs F-actin, in a Ca2+-dependent manner. Thus, the protein appeared functionally similar to the fragmin previously isolated fromPhysarum plasmodia (plasmodial fragmin). However, the two proteins had slightly different mobilities on urea-SDS-PAGE, and antibodies raised against the two proteins scarcely cross-reacted with each other. Hence, we conclude that the two proteins are closely related to but are different from each other, and we have named the novel protein ‘myxamoebal fragmin’. Immunoblot analysis indicated that myxamoebal and plasmodial fragmins are specifically present in amoebae and plasmodia, respectively. Results of immunofluorescence staining suggest that the synthesis of plasmodial fragmin is switched on coordinately with the synthesis of the heavy chain of plasmodial myosin and other plasmodium-specific contractile proteins during the apogamic differentiation of amoebae to plasmodia.

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Author notes

  1. Masaki Furuya

    Present address: Frontier Research Programs, Laboratory for Plant Biological Regulation, The Riken Institute, 351-01, Saitama, Japan

Authors and Affiliations

  1. Department of Biology, Faculty of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, 113, Tokyo, Japan

    Taro Q. P. Uyeda & Masaki Furuya

  2. Department of Molecular Biology, Faculty of Science, Nagoya University, 464, Nagoya, Japan

    Sadashi Hatano

  3. Department of Pharmacology, Faculty of Medicine, University of Tokyo, 113, Tokyo, Japan

    Kazuhiro Kohama

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  1. Taro Q. P. Uyeda
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  3. Kazuhiro Kohama
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Uyeda, T.Q.P., Hatano, S., Kohama, K. et al. Purification of myxamoebal fragmin, and switching of myxamoebal fragmin to plasmodial fragmin during differentiation ofPhysarum polycephalum . J Muscle Res Cell Motil 9, 233–240 (1988). https://doi.org/10.1007/BF01773893

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