Summary
Rat extensor digitorum longus (EDL) muscles were repeatedly frozen and thawed to kill completely all cellular constituents. Within four days, blood vessels and phagocytic cells invaded the muscles. Migrating or circulating myoblasts were not among the invading cells, and could be induced to invade the frozen muscle only when a physical bridge was created with an adjacent intact muscle. A further requirement for migration was that the connective tissue investments of both the frozen EDL and adjacent muscle had to be disrupted. This study demonstrates that regeneration of a muscle is primarily dependent upon the intrinsic satellite cell population, although under some circumstances recruitment of extrinsic cells is possible.
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Bateson, R. G., Woodrow, D. F. &Sloper, J. C. (1967) Circulating cells as a source of myoblasts in regenerating injured mammalian skeletal muscle.Nature, Lond. 213, 1035–6.
Benoit, P. W. &Belt, W. D. (1970) Destruction and regeneration of skeletal muscle after treatment with a local anesthetic, bupivacaine (Marcaine).J. Anal. 107, 547–56.
Bischoff, R. (1974) Enzymatic liberation of myogenic cells from adult rat muscle.Anat. Rec. 180, 645–62.
Ghins, E., Colson-Van Schoor, M. &Marechal, G. (1984) The origin of muscle stem cells in rat triceps surae regenerating after mincing.J. Musc. Res. Cell Motility 5, 711–22.
Grounds, M., Partridge, T. A. &Sloper, J. C. (1980) The contribution of exogenous cells to regenerating skeletal muscle: an isoenzyme study of muscle allografts in mice.J. Path. 132, 325–41.
Grounds, M. D. &Partridge, T. A. (1983) Isoenzyme studies of whole muscle grafts and movement of muscle precursor cells.Cell Tiss. Res. 230, 677–88.
Hall-Craggs, E. C. B. (1974) Rapid degeneration and regeneration of a whole skeletal muscle following treatment with bupivacaine (Marcaine).Expl Neurol. 43, 349–58.
Hansen-Smith, F. A. &Carlson, B. (1979) Cellular responses to free grafting of the extensor digitorum longus muscles of the rat.J. Neurol. Sci. 41, 149–73.
Hansen-Smith, F. M., Carlson, B. M. &Irwin, K. L. (1980) Revascularization of the freely grafted extensor digitorum longus muscle in the rat.Am. J. Anat. 158, 65–82.
Maltin, C. A., Harris, J. B. &Cullen, M. J. (1983) Regeneration of mammalian skeletal muscle following the injection of the snake-venom toxin, Taipoxin.Cell Tiss. Res. 232, 565–77.
Neerunjun, J. S. &Dubowitz, V. (1975) Identification of regenerated dystrophic minced muscle transplanted in normal mice.J. Neurol. Sci. 24, 33–8.
Partridge, T. A. &Sloper, J. C. (1977) A host contribution to the regeneration of muscle grafts.J. Neurol. Sci. 33, 425–35.
Rolston, J. L. I. (1970) Further studies on the orthotopic transplantation of skeletal mucle.Texas Rep. Biol. Med. 28, 97–104.
Schultz, E., Jaryszak, D. L. &Valliere, C. R. (1985) Response of satellite cells to focal skeletal muscle injury.Muscle & Nerve 8, 217–22.
Watt, D. J. (1982) Factors which affect the fusion of-allogenic muscle precursor cellsin vivo.Neuropath. appl. Neurol. 8, 135–47.
Watt, D. J., Lambert, K., Morgan, J. E., Partridge, T. A. &Sloper, J. G. (1982) Incorporation of donor muscle precursor cells into an area of muscle regeneration in the host muscle.J. Neurol. Sci. 57, 319–31.
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Schultz, E., Jaryszak, D.L., Gibson, M.C. et al. Absence of exogenous satellite cell contribution to regeneration of frozen skeletal muscle. J Muscle Res Cell Motil 7, 361–367 (1986). https://doi.org/10.1007/BF01753657
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DOI: https://doi.org/10.1007/BF01753657