Skip to main content
SpringerLink
Log in

Inhibition of mammary tumorigenesis in virgin rats by adoptive transfer of splenocytes from parous donors

  • Original articles
  • Published:
Cancer Immunology, Immunotherapy Aims and scope Submit manuscript

Summary

Splenocytes from parous rats have been previously found to have cytotoxic activity against mammary tumor cells in vitro. Experiments were carried out to determine if this pregnancy-induced cytotoxic nature of the splenocytes is inherent and transferable. Splenocytes from parous rats were adoptively transferred to a group of virgin rats. Another group of age-matched, virgin rats received splenocytes from virgin donors in a similar way. After a period of rest, at the age of 55 days, the rats belonging to both of the groups, received 7,12-dimethylbenz(a)anthracene (DMBA) intragastrically. A third group of untreated virgin rats were also given the chemical carcinogen the same way as above and were considered as intact controls. The rats were monitored for development and growth of mammary tumor from 60 days of DMBA administration. After 4 months of DMBA administration the rats were sacrificed and mammary glands were examined for tumors. Mammary glands with no visible tumors were taken for whole mount preparation, to be examined for microscopic lesions. The results showed that 33 of 41 intact control rats, developed tumor and 27 of the 34 rats that received spleen cells from virgin rats developed tumors. Of the rats that received spleen cells from parous rats, only 18 out of 37 rats developed tumors, indicating an inhibition of tumor induction in these rats. Growth rate of the tumors in this group was also slower than in the control groups.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Brenner RD, Nisbet AD, Herriot R, Horne CHW, McArdle C, Crawford D, Bohn H (1981) Detection of Placental Protein Five (pps) and Pregnancy Specific Glycoprotein (SPI) in Benign and Malignant Breast Disease. Onco Develop Biol Med 2: 55

    Google Scholar 

  2. Chakravarty PK, Ghosh SK, Sinha DK (1991) Pregnancy-induced cytotoxicity of splenocytes against mammary tumor cells in rat. Oncology (in press)

  3. Crawford V, Pim DC, Bulbrook RD (1982) Detection of antibodies against the cellular protein P53 in sera from patients with breast cancer. Cancer 30: 403

    Google Scholar 

  4. Crum ED (1980) Lymphocytes that mediate systemic resistance to in vivo growth of a carcinogen-induced syngenic tumor. Int J Cancer 26: 53

    PubMed  Google Scholar 

  5. Dao TL, Pazik J (1981) Early pregnancy protects against mammary gland carcinogenesis. Proc Am Assoc Cancer Res 22: 96

    Google Scholar 

  6. Durie BGM, Salmon SE (1975) High speed scintillation autoradiography. Science 190: 1093

    PubMed  Google Scholar 

  7. Forsman LM, Jouppila PI, Andersson LC (1984) Sera from multiparous women containing antibodies mediating cytotoxicity against breast carcinoma cells. J Immunol 19: 135

    Google Scholar 

  8. Corcznski RM (1978) Response of tumor-related and normal lymphocytes to antigen on fibroblasts from embryos of varying age. Br J Cancer 37: 780

    PubMed  Google Scholar 

  9. Gorcznski RM, MacRae S (1982) Retardation and promotion of growth of spontaneous by appearing tumors using immune lymphocytes previously exposed to embryonic antigens. Cancer Res 42: 784

    PubMed  Google Scholar 

  10. Grudzinska J, Coombes R, Ratcliffe JG, Gordon YB, Powles TJ, Munro Neville A, Chard T (1980) Circulating levels of pregnancy specific A-1 glycoprotein in patients with testicular, Bronchogenic and breast carcinomas. Cancer 45: 102

    PubMed  Google Scholar 

  11. Haller O, Hansson M, Kiessling R, Wigzell H (1977) Role of nonconventional natural killer cells in resistance against syngeneic tumor cells in vivo. Nature 270: 609

    PubMed  Google Scholar 

  12. Herberman RB, Holden H (1978) Natural cell mediated immunity. Adv Cancer Res 27: 305

    PubMed  Google Scholar 

  13. Kiessling R, Wigzell H (1979) An analysis of the murine NK cells as to structure, function and biological relevance. Immunol Rev 44: 165

    PubMed  Google Scholar 

  14. Kuperman O, Fortner GW, Lucas ZJ (1975) Immune response to a syngeneic mammary adenocarcinoma III. Development of memory and suppressor functions modulating cellular cytotoxicity. J Immunol 115: 1282

    PubMed  Google Scholar 

  15. Monteiro J, Biswass Al-Awquati MA, Greening WP, McKinn JA, Neville AM (1982) Serum levels of human placental lactogen and pregnancy-specific Al glycoprotein in breast cancer. Br J Cancer 46: 279

    PubMed  Google Scholar 

  16. Moore DH, Moore DH 2nd, Moore CT (1983) Breast carcinoma etiological factors. Adv Cancer Res 40: 189

    PubMed  Google Scholar 

  17. Rhodes HM, Overall JE (1982) Quantitative methods. Psychol Bull 91: 418

    Google Scholar 

  18. Russo J, Russo IH (1980) Susceptibility of the mammary gland to carcinogenesis: II. Pregnancy interruptions a risk factor in tumor incidence. Am J Pathol 100: 497

    PubMed  Google Scholar 

  19. Russo J, Russo IH (1987) Biological and molecular bases of mammary carcinogenesis. Lab Invest 57: 112

    PubMed  Google Scholar 

  20. Sarcione EJ, Dellumo D, Zloty M (1983) Pregnancy associated alpha-2 glycoprotein (PAG) synthesis by human breast cancer tissue and cultured cell lines. Int J Cancer 31: 143

    PubMed  Google Scholar 

  21. Sinha DK (1981) Growth of mammary glands from monodisperesed cells and susceptibility to 7,12-dimethylbenz[a]anthracene. Cancer Lett 12: 111

    PubMed  Google Scholar 

  22. Sinha DK, Pazik JE, Dao TL (1988) Prevention of mammary carcinogenesis in rats by pregnancy: effect of full-term and interrupted pregnancy. Br J Cancer 57: 390

    PubMed  Google Scholar 

  23. Warner NL, Woodruff MFA, Burton RC (1977) Inhibition of the growth of lymphoid tissue in syngeneic athymic (nude) mice. Int J Cancer 20: 146

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Breast Surgery and Breast Cancer Research Unit, Roswell Park Memorial Institute, 666 Elm Street, 14263, Buffalo, NY, USA

    Prabir K. Chakravarty & Dilip K. Sinha

Authors
  1. Prabir K. Chakravarty
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Dilip K. Sinha
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This research was supported by USPHS grant CA 3613906 awarded by the National Cancer Institute

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chakravarty, P.K., Sinha, D.K. Inhibition of mammary tumorigenesis in virgin rats by adoptive transfer of splenocytes from parous donors. Cancer Immunol Immunother 33, 263–266 (1991). https://doi.org/10.1007/BF01744946

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01744946

Key words

Search

Navigation