Summary
The neurotransmitter, noradrenaline, is inactivated by active transport out of the synaptic cleft, either back into the adrenergic neurons (uptake1) or into extraneuronal cells (uptake2). Although experimental models clearly demonstrate uptake2 in various animal tissues, there are few reports of studies that demonstrate uptake2 in human tissue. Furthermore, uptake2 has never been described in human heart tissue. We demonstrated a reduction of3H-noradrenaline uptake in human atrial and venous tissue and in rat atrial and ventricular tissue by O-methylisoprenaline — a known inhibitor of uptake2. These results indicate that uptake2, as has been described in previous experimental models, does exist in humans and further, the results emphasize the importance of uptake2, since about 50% of exogenous noradrenaline is inactivated by uptake2 in the rat heart.
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References
Graefe K-H, Bönisch H (1988) The transport of amines across the axonal membranes of noradrenergic and dopaminergic neurones. In: Trendelenburg U, Weiner N (eds) Catecholamines I (Handbook Exp Pharmacol, vol 90). Springer, Berlin Heidelberg New York London Paris Tokyo, pp 193–245
Iversen LL (1965) The uptake of catecholamines at high perfusion concentrations in the rat isolated heart: A novel catecholamine uptake process. Br J Pharmacol 25:18–33
Gillespie JS, Towart R (1973) Uptake kinetics and ion requirements for extraneuronal uptake of noradrenaline by arterial smooth muscle and collagen. Br J Pharmacol 47:556–567
Trendelenburg U (1988) The extraneuronal uptake and metabolism of catecholamines. In: Trendelenburg U, Weiner N (eds) Catecholamines I (Handbook Exp Pharmacol, vol 90/1). Springer, Berlin Heidelberg New York London Tokyo, pp 279–319
Burnstock G, McCulloch MW, Story DF, Wright ME (1972) Factors affecting the extraneuronal inactivation of noradrenaline in cardiac and smooth muscle. Br J Pharmacol 46:243–253
Chang PC, Krogt van der JA, Brummelen van P (1987) Demonstration of neuronal and extraneuronal uptake of circulating norepinephrine in the forearm. Hypertension 9:647–653
Schömig E, Schönfeld C-L (1990) Extraneuronal noradrenaline transport (uptake2) in a human cell line (Caki-1 cells). Naunyn Schmiedebergs Arch Pharmacol 341:404–410
Schömig E, Babin-Ebell J, Russ H, Trendelenburg U (1992) The force driving the extraneuronal transport mechanism for catecholamines (uptake2). Naunyn Schmiedebergs Arch Pharmacol 345(4):437–443
Fiebig ER, Trendelenburg U (1978) The neuronal and extraneuronal uptake and metabolism of3H-(−)-noradrenaline in the perfused rat heart. Naunyn Schmiedebergs Arch Pharmacol 303:21–35
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Babin-Ebell, J., Gliese, M. Extraneuronal uptake of noradrenaline in human tissue (uptake2). Heart Vessels 10, 151–153 (1995). https://doi.org/10.1007/BF01744482
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DOI: https://doi.org/10.1007/BF01744482