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Induction of interleukin-2 receptor by tumor necrosis factor α on cultured ovarian tumor-associated lymphocytes

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Summary

We have recently reported that autologous tumor-specific cytotoxic T lymphocyte (CTL) lines and clones can be developed from lymphocytes infiltrating ovarian malignant ascites (TAL). In this study, we investigated the biological effects of tumor necrosis factor α (TNFα) in the induction, expansion, long-term proliferation and lytic function of CD8+ TAL. TNFα up-regulated the IL-2 receptor (IL-2R) α chain (Tac antigen) on the surface of CD3+ CD8+ CD4− TAL, enhanced the proliferation of autologous tumor-specific CTL, and potentiated their lytic function in long-term cultures. Furthermore, in the induction and expansion phase of CD8+ TAL, the presence of TNFα was associated with a selective increase in CD8+ IL-2R+ (Tac+) cells, and subsequent decrease in CD4+ IL-2R+ (Tac+) cells. These results suggest that the observed facilitation of the outgrowth of CD8+ cells in TAL cultures may be due, at least in part, to the up-regulation of IL-2R, and indicate the usefulness of TNFα in the analysis of signalling in autologous tumor-reactive CTL.

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Authors and Affiliations

  1. Department of Gynecology, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Box 67, 77 030, Houston, Texas, USA

    Constantin G. Ioannides, Bryan Fisk, Barbara Tomasovic & Ralph S. Freedman

  2. Cytokine Research Laboratory, Department of Clinical Immunology and Biological Therapy, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, 77 030, Houston, Texas, USA

    Raj Pandita & Bharat B. Aggarwal

Authors
  1. Constantin G. Ioannides
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  2. Bryan Fisk
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  3. Barbara Tomasovic
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  4. Raj Pandita
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  5. Bharat B. Aggarwal
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  6. Ralph S. Freedman
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Ioannides, C.G., Fisk, B., Tomasovic, B. et al. Induction of interleukin-2 receptor by tumor necrosis factor α on cultured ovarian tumor-associated lymphocytes. Cancer Immunol Immunother 35, 83–91 (1992). https://doi.org/10.1007/BF01741854

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  • DOI: https://doi.org/10.1007/BF01741854

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