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Activation and expansion of tumour-infiltrating lymphocytes by anti-CD3 and anti-CD28 monoclonal antibodies

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Summary

Cytotoxic T lymphocytes from healthy donors can be expanded to high numbers from the peripheral blood using combinations of anti-CD3 and anti-CD28 monoclonal antibodies (mAb). We investigated whether these antibodies could also be used to induce outgrowth of tumour-infiltrating lymphocytes (TIL) from tumour tissue. In the initiation phase of TIL culture immobilized anti-CD3 antibodies together with anti-CD28 mAb and low-dose interleukin-2 induced a rapid expansion of T cells from various human tumour tissues. The cultured cells showed high levels of cytotoxic T lymphocyte activity, but low levels of lymphokine-activated killer cell activity were generated. This study shows that TIL can be efficiently expanded from tumour tissue by combinations of anti-CD3 and anti-CD28 antibodies. This protocol for cell expansion in vitro may substantially reduce the time required to reach sufficient numbers of TIL for re-infusion to the patient.

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Authors and Affiliations

  1. c/o Publication Secretariat, Central Laboratory of the Netherlands Red Cross Blood Transfusion Service and Laboratory for Experimental and Clinical Immunology of the University of Amsterdam, P. O. Box 9406, 1006 AK, Amsterdam, The Netherlands

    Erik W. P. Nijhuis & René A. W. van Lier

  2. Dutch Cancer Institute, Amsterdam, The Netherlands

    Elly v/d Wiel-van Kemenade & Carl G. Figdor

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  1. Erik W. P. Nijhuis
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  2. Elly v/d Wiel-van Kemenade
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  3. Carl G. Figdor
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  4. René A. W. van Lier
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Nijhuis, E.W.P., v/d Wiel-van Kemenade, E., Figdor, C.G. et al. Activation and expansion of tumour-infiltrating lymphocytes by anti-CD3 and anti-CD28 monoclonal antibodies. Cancer Immunol Immunother 32, 245–250 (1990). https://doi.org/10.1007/BF01741708

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  • DOI: https://doi.org/10.1007/BF01741708

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