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DMNH — Ein neuer kompetenter Indikator für die Messung der Chemilumineszenz von neutrophilen Granulocyten und Monocyten

DMNH — A new sensitive indicator for measuring the chemiluminescence of granulocytes and monocytes

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Summary

Comparative studies on the effects of the luminescence indicators 5-Amino-2,3-dihydro-1,4-phthalazinedione (Luminol, LUM) and 7-Dimethylamino-naphthalene-1,2-dicarbonic-acidhydracide (DMNH) in measuring the chemiluminescence of ingesting and non-ingesting polymorphonuclear leukocytes (PMN) and monocytes (MOC) are described. DMNH is more effective than LUM in measuring chemiluminescence of PMN and MOC under both biological conditions. Indications are given that LUM inhibits the oxidative metabolism of both cell types. The use of DMNH as a luminescence indicator makes possible the research of cells with low activity of the oxidative metabolism in small numbers.

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Abbreviations

CL:

Chemilumineszenz

DMNH:

7-dimethylamino-naphthalin-1,2-dicarbonsäure-hydrazid

DMSO:

Dimethylsulfoxid

LUM:

Luminol, 5-amino-2,3-dihydro-1,4-phthalazinedion

MOC:

Monocyten

PBS:

phosphatgepufferte NaCl-Lösung (p h 7.2)

PMN:

neutrophile Granulocyten

RLU:

relative Lichteinheiten

Literatur

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  4. Eschenbach C, Hahm W, Adrian U (1985) deDMNH-Chemilumineszenz ingestierender Monocyten. Klin Wochenschr 63:364–370

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  5. Wulff K (1984) Persönliche Mitteilung

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Authors and Affiliations

  1. Medizinisches Zentrum für Kinderheilkunde der Philipps-Universität Marburg/Lahn, Germany

    C. Eschenbach & U. Adrian

Authors
  1. C. Eschenbach
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  2. U. Adrian
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Additional information

Die Untersuchungen wurden mit Sachbeihilfen der Deutschen Forschungsgemeinschaft durchgeführt (Es 26/5-1)

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Eschenbach, C., Adrian, U. DMNH — Ein neuer kompetenter Indikator für die Messung der Chemilumineszenz von neutrophilen Granulocyten und Monocyten. Klin Wochenschr 63, 1218–1225 (1985). https://doi.org/10.1007/BF01733781

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  • DOI: https://doi.org/10.1007/BF01733781

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