Summary
Nephrectomy of mature male rats was found to result in a significant increase in the circulatory half-life of tritiated ovine lutropin. The interaction of the glycoprotein hormone with the kidneys was studied in a more direct fashion using electron microscopic autoradiography. Evidence is presented showing the transfer of the hormone from microvilli into tubular epithelia (probably via vesicular transport), where radioactivity then becomes associated with lysosomes. This provides direct support for related results based on subcellular fractionation in which renal lysosomal catabolism was suggested as being important in the degradation of tritiated lutropin (M. Ascoli,R. A. Liddle, andD. Puett, Molecular and Cellular Endocrinology 4, 297, 1976). These results add substantial weight to the growing evidence that the kidneys assume a major role in controlling the concentration of circulating macromolecules.
References
Ascoli, M., Liddle, R. A. and Puett, D., 1975. Mol. Cell. Endocrin. 3, 21–36.
Ascoli, M., Liddle, R. A. and Puett, D., 1976. Mol. Cell. Endocrin. 4, 297–310.
Puett, D., Ascoli, M. and Holladay, L. A., 1974. Hormone Binding and Target Cell Activation in the Testis (M. L. Dufau and A. R. Means, eds.), pp. 109–124, Plenum Press, New York.
Ascoli, M. and Puett, D., 1976. Endocrinology 99, 1237–1243.
Holladay, L. A. and Puett, D., 1975. Arch. Biochem. Biophys. 171, 708–720.
Ascoli, M. and Puett, D., 1974. Biochim. Biophys. Acta 371, 203–210.
Ascoli, M. and Puett, D., 1976. Endocrinology 99, 1229–1236.
Spurr, A. R., 1969. J. Ultrastruct. Res. 26, 31–43.
Caro, L. G. and Van Tubergen, R. P., 1962. J. Cell Biol. 15, 173–188.
Karnovsky, M. J., 1961. J. Biophys. Biochem. Cytol. 11, 729–732.
Hay, E. D. and Revel, J. P., 1963. Dev. Biol. 7, 152–168.
Gay, V. L., 1974. Endocrinology 95, 1582–1588.
De Kretser, D. M., Atkins, R. C. and Paulsen, C. A., 1973. J. Endocrinol. 58, 425–434.
Stacy, B. D., Wallace, A. L. C., Gemmell, R. T. and Wilson, B. W., 1976. J. Endocrinol. 68, 21–30.
Maunsbach, A. D., 1969. Lysosomes in Biology and Pathology, Vol. 1 (J. T. Dingle and H. B. Fell, eds.), pp. 115–154, North Holland Publ. Co., Amsterdam.
Author information
Authors and Affiliations
Additional information
Research Career Development Awardee (AM-00055).
Rights and permissions
About this article
Cite this article
Robinson, J.P., Derreberry, S., Liddle, R.A. et al. Renal uptake of lutropin. Studies based on electron microscopic autoradiography and nephrectomy. Mol Cell Biochem 15, 63–66 (1977). https://doi.org/10.1007/BF01731289
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01731289