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Differentiation of strains from a food-borne outbreak ofSalmonella enterica by phenotypic and genetic typing methods

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Abstract

A combination of typing methods was used to identify the strains and the infection source in a food-borne outbreak ofSalmonella enterica occurring in a summer camp and affecting 25 children. All isolates tested were found to be serovar Enteritidis, with an identical biotype API 20E and ribotype. However, they differed in their plasmid profiles and/or antibiograms, and were grouped into three strains. One strain was found in human stools, another in a hen's egg, and the third in both stools and another egg pointing to large Spanish omelettes to be the contaminated food source.

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References

  1. Ballows A. Manual of clinical microbiology, 5th ed. Washington, DC: ASM, 1991.

    Google Scholar 

  2. Pascual MR. Microbiologia alimentaria: Metodología anal ítica para alimentos y bebidas. Madrid: Diaz de Santos, 1992.

    Google Scholar 

  3. National Committee for Clinical Laboratory Standards. Approved standard M2-A3: Performance standards for antimicrobial disk susceptibility tests. Vilanova, PA: NCCLS, 1984.

    Google Scholar 

  4. Alonso R, et al. Attempts to establish phage typing as epidemiological marker forSalmonella enteritidis. Ann Inst Pasteur Microbiol 1987; 138: 579–585.

    Google Scholar 

  5. Deneer HG, Boychuck I. Species-specific detection ofListeria monocytogenes by DNA amplification. App Environ Microbiol 1991; 57: 606–609.

    Google Scholar 

  6. Sambrook J, Fritch EF, Maniatis T. Molecular cloning: A laboratory manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, 1989.

    Google Scholar 

  7. Brosius J, et al. Construction and fine mapping of recombinant plasmids containing therrnB ribosomal RNA operon ofEscherichia coli. Plasmid 1981; 6: 112–118.

    Google Scholar 

  8. Lujan R, et al. Plasmid profiles as an epidemiological marker forSalmonella enterica serotype Enteritidis foodborne outbreaks. Microbiología SEM 1990; 6: 45–50.

    Google Scholar 

  9. Ribera MJ, et al. Molecular and epidemiological study ofSalmonella clinical isolates. J Clin Microbiol 1991; 29: 927–932.

    Google Scholar 

  10. Nakamura M, et al. Possible relationship of a 36-MegadaltonSalmonella enteritidis plasmid to virulence in mice. Infect Immun 1985; 47: 831–833.

    Google Scholar 

  11. Gonzalez-Hevia MA, Llaneza J, Mendoza MC. Usefulness of molecular genetic markers in the typing ofSalmonella enterica serovar Enteritidis causing a food-borne outbreak. Int J Food Microbiol 1994; 22: 97–103.

    Google Scholar 

  12. Anonymous. Vigilancia de brotes de infecciones e intoxicaciones de origen alimentario: España, años 1990–92. Bol Epidem Microbiol 1993; 162–167.

  13. Anonymous. Estado de las enfermedades de declaración obligatoria: Resultados de la declaración al Sistema de Información Microbiológica. Bol Epidem Microbiol 1993; 1: 249–272.

  14. Martinetti G, Altwegg M. rRNA gene restriction patterns and plasmid analysis as a tool for typingSalmonella Enteritidis. Res Microbiol 1990; 141: 1151–1162.

    Google Scholar 

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Hevia, M.A.G., Mendoza, M.C. Differentiation of strains from a food-borne outbreak ofSalmonella enterica by phenotypic and genetic typing methods. Eur J Epidemiol 11, 479–482 (1995). https://doi.org/10.1007/BF01721237

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  • DOI: https://doi.org/10.1007/BF01721237

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