Abstract
Ten humanCoxiella burnetii isolates from french patients with acute hepatitis or chronic endocarditis were characterized according to their polymorphism in DNA restriction patterns and differentiated by plasmid-specific PCR. The aim of this investigation was to clarify if the present classification of so called ‘acute’ and ‘chronic’Coxiella burnetii isolates — based on plasmid profile of a so far limited number of partly ancient isolates — could be confirmed with lately isolated organisms of this agent. The data obtained in this investigation indicate that this classification based onC. burnetii plasmid content is no longer justified.
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References
Frazier ME, Mallavia LP, Samuel JE, Baca OG. DNA probes for the identification ofCoxiella burnetii strains. In: Rickettsiology, current issues and perspectives. NY Acad Sci 1990; 590: 445–458.
Hendrix LR, Samuel JE, Mallavia LP. Differentiation ofCoxiella burnetii isolates by analysis of restriction-endonuclease-digested DNA separated by SDS-PAGE. J Gen Microbiol 1991; 137: 269–276.
Mallavia LP, Samuel JE. Genetic diversity ofCoxiella burnetii. In: Moulder J, ed, Intracellular Parasitism. Boca Raton: CRC Press, 1989: 117–126.
Samuel JE, Frazier ME, Mallavia LP. Correlation of plasmid type and disease caused byCoxiella burnetii. Infect Immun 1985; 49: 775–779.
Samuel JE, Frazier ME, Mallavia LP. Stability of plasmid sequences in an acute Q-fever strain ofCoxiella burnetii. Gen Microbiol 1988; 134: 1795–1805.
Savinelli EA, Mallavia LP. Comparison ofCoxiella burnetii plasmids to homologous chromosomal sequences present in a plasmidless endocarditis-causing isolate. In: Rickettsiology, current issues and perspectives. Ann NY Acad Sci 1990; 590: 523–533.
Thiele D, Willems H, Köpf G, Krauss H. Polymorphism in DNA restriction patterns ofCoxiella burnetii isolates investigated by pulsed field gel electrophoresis and image analysis. Eur J Epidemiol 1993; 9: 419–425.
Valková D. A study of plasmid types ofCoxiella burnetii. Thesis (Biology). Bratislava, 1993 (in Czech).
Vodkin MH, Williams JC, Stephenson EH. Genetic heterogeneity among isolates ofCoxiella burnetii. J Gen Microbiol 1986; 132: 455–463.
Willems H, Thiele D, Krauss H. Plasmid based differentiation and detection ofCoxiella burnetii in clinical samples. Eur J Epidemiol 1993; 9: 411–418.
Stein A, Raoult D. Lack of pathotype specific gene in humanCoxiella burnetii isolates. Microbial Pathogen 1993; 15: 177–185.
Raoult D, Vestris G, Enea M. Isolation of 16 strains ofCoxiella burnetii from patients by using a sensitive centrifugation cell culture system and establishment of strains in HEL cell. J Clin Microbiol 1990; 28: 2482–2484.
Arens M. Continuous multiplication ofCoxiella burnetii through persisting infection in buffalo green monkey (BGM) cell cultures. Vet Med 1983; Series B, 30: 109–116.
Sawadogo M, van Dyke MM. A rapid method for the purification of deprotected oligodeoxynucleotides. Nucl Acids Res 1993; 39: 674.
Hoover TA, Vodkin MH, Williams JC. ACoxiella burnetii repeated DNA element resembling a bacterial insertion sequence. J Bacteriol 1992; 174: 5540–5548.
Chomczynski P. One-hour downward alkaline capillary transfer for blotting of DNA and RNA. Anal Biochem 1992; 201: 134–139.
Zhi N, Yu S-R, Yu GQ, Zhang X. Molecular characterization of cloned variants ofCoxiella burnetii isolated in China. Acta Virol 1991; 35: 173–183.
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Thiele, D., Willems, H. Is plasmid based differentiation ofCoxiella burnetii in ‘acute’ and ‘chronic’ isolates still valid?. Eur J Epidemiol 10, 427–434 (1994). https://doi.org/10.1007/BF01719667
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DOI: https://doi.org/10.1007/BF01719667