Abstract
Non-H-2 genes responsible for negative selection of Tcrb-V 11+ T cells were examined using backcross mice of various strains with C58, which does not delete Tcrb-V 11+ T cells. Two independently segregating genes were found: one leading to partial deletion was closely linked toLy-2/Ly-3 on chromosome 6, and the second giving virtually complete deletion has not yet been mapped. The A strain had only the former, whereas BALB/c, BALBK, B10.BR, CBA-T6, C3H/He, and DBA/2 expressed both of these genes. Although a gene(s) of the NIH strain led only to partial deletion, the chromosomal localization of the gene(s) has not yet been determined: no informative polymorphic molecules are expressed from genes on chromosome 6 of this strain.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bill, J., Kanagawa, O., Woodland, D. L., and Palmer, E.: The MHC molecule I-E is necessary but not sufficient for the clonal deletion of Vβ11-bearing T cells.J Exp Med 169: 1405–1419, 1989
Davisson, M. T., Roderick, T. H., Hillyard, A. L., and Doolittle, D. P.: The locus map of the mouse.Mouse News Letter 84: 15–23, 1989
Gao, E. K., Kanagawa, O., and Sprent, J.: Capacity of unprimed CD4+ and CD8+ T cells expressing Vβ11 receptors to respond to I-E alloantigens in vivo.J Exp Med 170: 1947–1957, 1989
Gorman, S. D., Sun, Y. H., Zamoyska, R., and Parnes, J. R.: Molecular linkage of the Ly-3 and Ly-2 genes. Requirement of Ly-2 for Ly-3 surface expression.J Immunol 140: 3646–3653, 1988
Happ, M. P., Woodland, D. L., and Palmer, E.: A third T-cell receptor β-chain variable region gene encodes reactivity toMls-l a gene products.Proc Natl Acad Sci USA 86: 6293–6296, 1989
Kappler, J. W., Roehm, N., and Marrack, P.: T cell tolerance by clonal elimination in the thymus.Cell 49: 273–280, 1987
Kappler, J. W., Staerz, U., White, J., and Marrack, P. C.: Selftolerance eliminates T cells specific for Mls-modified products of the major histocompatibility complex.Nature 332: 35–40, 1988
Ledbetter, J. A. and Herzenberg, L. A.: Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens.Immunol Rev 47: 63–90, 1979
Love, J. M., Knight, A. M., McAleer, M. A., and Todd, J. A.: Towards construction of a high resolution map of the mouse genome using PCR-analysed microsatellites.Nucleic Acids Res 18: 4123–4130, 1990
MacDonald, H. R., Schneider, R., Lees, R. K., Howe, R. C., AchaOrbea, H., Festenstein, H., Zinkernagel, R. M., and Hengartner, H.: T-cell receptor Vβ use predicts reactivity and tolerance to Mlsa-encoded antigens.Nature 332: 40–45, 1988
Marrack, P. and Kappler, J.: T cells can distinguish between allogeneic major histocompatibility complex products on different cell types.Nature 332: 840–843, 1988
Okada, C. Y., Holzmann, B., Guidos, C., Palmer, E., and Weissman, I. L.: Characterization of a rat monoclonal antibody specific for a determinant encoded by the Vß7 gene segment. Deletion of Vß7+ T cells in mice with Mls-1a haplotype.J Immunol 144: 3473–3477, 1990
Pullen, A. M., Marrack, P., and Kappler, J. W.: The T cell repertoire is heavily influenced by tolerance to polymorphic self-antigens.Nature 335: 795–801, 1988
Sarmiento, M., Glasebrook, A. L., and Fitch, F. W.: IgG or IgM monoclonal antibodies reactive with different determinants on the molecular complex bearing Lyt2 antigen block T cell-mediated cytolysis in the absence of complement.J Immunol 125: 2665–2672, 1980
Tomonari, K.: Tolerance in vivo and in vitro.Immunol Rev 116:139–157, 1990a
Tomonari, K.: Linkage between Tcrb-V and a gene responsible for deletion of Tcrb-V11+ T cells.Immunogenetics 32: 60–62, 1990b
Tomonari, K. and Lovering, E.: T-cell receptor-specific monoclonal antibodies against a Vß11-positive mouse T-cell clone.Immunogenetics 28: 445–451, 1988
Tomonari, K. and Spencer, S.: Epitope specific binding of CD8 regulates activation of T cells and induction of cytotoxicity.Int Immunol 2, in press, 1990
Tomonari, K., Lovering, E., and Spencer, S.: Correlation between the Vß4+CD8+ T-cell population and the H-2d haplotype.Immunogenetics 31: 333–339, 1990
Vacchio, M. S., Ryan, J. J., and Hodes, R. J.: Characterization of the ligand(s) responsible for negative selection of Vβ 11- and Vβ 12-expressing T cells: effect of a new Mls determinant.J Exp Med 172: 807–813, 1990
Woodland, D., Happ, M. P., Bill, J., and Palmer, E.: Requirement for co-tolerogenic gene products in the clonal deletion of I-E reactive T cells.Science 247: 964–967, 1990
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tomonari, K., Fairchild, S. The genetic basis of negative selection of Tcrb-Vll+ T cells. Immunogenetics 33, 157–162 (1991). https://doi.org/10.1007/BF01719234
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01719234