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Expression and characterization of equine herpesvirus 1 glycoprotein D in mammalian cell lines

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Summary

Equine herpesvirus 1 glycoprotein D (EHV-1 gD) expressed constitutively in mammalian cell lines had similar electrophoretic mobility to gD produced in EHV-1 infected cells but lacked a possibly complexed higher molecular weight form seen in the latter. Recombinant gD was N-terminally cleaved at the same site as gD in EHV-1 infected cells and expression was associated with enhanced levels of cell-cell fusion, indicating a role for EHV-1 gD in cell-to-cell transmission of virus.

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Authors and Affiliations

  1. School of Biological Sciences, Macquarie University, Sydney

    J. E. Wellington, G. L. Lawrence & J. M. Whalley

  2. Department of Veterinary Pathology, University of Sydney, New South Wales, Australia

    D. N. Love

Authors
  1. J. E. Wellington
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  2. G. L. Lawrence
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  3. D. N. Love
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  4. J. M. Whalley
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Wellington, J.E., Lawrence, G.L., Love, D.N. et al. Expression and characterization of equine herpesvirus 1 glycoprotein D in mammalian cell lines. Archives of Virology 141, 1785–1793 (1996). https://doi.org/10.1007/BF01718301

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  • DOI: https://doi.org/10.1007/BF01718301

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