Summary
Ten presumptive enterovirus isolates which could not be neutralized by type specific antisera to any prototype enterovirus were related to echovirus 22 using molecular, biologic and serologic methods. Viral protein fingerprinting and PCR first suggested that these strains were variants of echovirus 22. Three of the strains were echovirus 22 prime strains, i.e., antiserum made to the variant strain neutralized the variant and the prototype strain. The other strains were neutralized by antiserum to the prime strains. Unlike typical enteroviruses, echovirus 22 and 23 prototype viruses and 7 of the 10 variants were heat stable at 50 °C in H2O for 1 h.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Chapman NM, Tracy S, Gauntt CJ, Fortmueller U (1990) Molecular detection and identification of enteroviruses using enzymatic amplification and nucleic acid hybridization. J Clin Microbiol 28: 843–850
Coller B, Chapman NM, Beck MA, Pallansch MA, Gauntt CJ, Tracy SM (1990) Echovirus 22 is an atypical enterovirus. J Virol 64: 2692–2701
Connor JD, Walpita P (1989) Identification of herpes simplex viruses by automated profile analysis of viral proteins. J Virol Methods 24: 245–252
Ehrnst A, Eriksson M (1993) Epidemiological features of type 22 echovirus infection. Scand J Infect Dis 25: 278–281
Grandien M, Forsgren M, Ehrnst A (1989) Enteroviruses and reoviruses. In: Schmidt NJ, Emmons RW (eds) Diagnostic procedures for viral, rickettsial and chlamydial infections, 6th ed. American Public Health Association, Washington, p 521
Gwaltney JM, Colonno RJ, Hamparian VV, Turner RB (1989) Rhinoviruses. In: Schmidt NJ, Emmons RW (eds) Diagnostic procedures for viral, rickettsial and chlamydial infections, 6th ed. American Public Health Association, Washington, pp 602–603
Hyypia T, Horsnell C, Maaronen M, Kahn M, Kalkkinen N, Auvinen P, Kinnunen L, Stanway G (1992) A distinct picornavirus group identified by sequence analysis. Proc Natl Acad Sci USA 89: 8847–8851
Rotbart HA (1990) Enzymatic RNA amplification of the enteroviruses. J Clin Microbiol 28: 438–442
Schmidt NJ, Lennette EH (1970) Antigenic variants of coxsackievirus type A24. Am J Epidemiol 91: 99–109
Schmidt NJ, Lennette EH, Ho HH (1966) Observations on antigenic variants of echovirus type 11. Proc Soc Exp Biol Med 123: 696–700
Schmidt NJ, Lennette EH, Ho HH (1974) An apparently new enterovirus isolated from patients with disease of the central nervous system. J Infect Dis 129: 304–307
Shimizu C, Rambaud C, Cheron G, Rouzioux C, Lozinski GM, Rao A, Stanway G, Krous HF, Burns JC (1996) Molecular identification of viruses in sudden infant death associated with myocarditis and pericarditis. Ped Infect Dis (in press)
Stanway G, Kalkkinen N, Roivainen M, Ghazi F, Khan M, Smyth M, Meurman O, Hyypia T (1994) Molecular and biologic characteristics of echovirus 22, a representative of a new picornavirus group. J Virol 68: 8232–8238
Wallis C, Melnick JL (1962) Cationic stabilization — a new property of enteroviruses. Virology 16: 504–506
Walpita P, Connor JD, Pfeifer D (1988) Protein fingerprinting: a novel virus identification system. J Virol Methods 24: 315–324
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Schnurr, D., Dondero, M., Holland, D. et al. Characterization of echovirus 22 variants. Archives of Virology 141, 1749–1758 (1996). https://doi.org/10.1007/BF01718297
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01718297