Summary
Thein vitro effect of subinhibitory and inhibitory concentrations of ofloxacin and G-CSF on the bactericidal activity of polymorphonuclear leucocytes (PMNL) againstEscherichia coli was investigated. PMNL obtained from healthy volunteers were incubated with different concentrations of G-CSF and ofloxacin for 180 min. The minimum inhibitory concentration (MIC) of ofloxacin and even 1/4 × MIC enhanced the bactericidal activity of PMNL. G-CSF at a concentration of 6,000 units/ml led to a significant improvement of the bactericidal activity of PMNL. The combination of 6,000 units/ml of G-CSF and ofloxacin in inhibitory as well as subinhibitory concentrations, however, showed a significant synergistic effect on the antibacterial activity of PMNL during the complete incubation period. Combinations of G-CSF and antibiotics could therefore be beneficial for infected patients, especially those with impaired cellular host defense.
Zusammenfassung
Wir untersuchtenin vitro die bakterizide Wirkung von neutrophilen Granulozyten in Kombination mit subinhibitorischen und inhibitorischen Konzentrationen von Ofloxacin und G-CSF aufEscherichia coli. Neutrophile Granulozyten wurden dazu zusammen mit unterschiedlichen Konzentrationen von G-CSF und Ofloxacin inkubiert. Die minimale Hemmkonzentration und sogar ein Viertel der minimalen Hemmkonzentration von Ofloxacin verstärkten die bakterizide Aktivität, 6.000 Einheiten/ml G-CSF führten zu einer signifikanten Verbesserung der bakteriziden Wirkung der neutrophilen Granulozyten. Die Kombination von 6.000 Einheiten/ml von G-CSF mit Ofloxacin in inhibitorischen und subinhibitorischen Konzentrationen zeigten einen synergistischen Effekt während der gesamten Inkubationszeit auf neutrophile Granulozyten. Die Gabe von G-CSF in Kombination mit Antibiotika könnte daher bei Infektionen, insbesondere bei immunsupprimierten Patienten, vorteilhaft sein.
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Kropec, A., Lemmen, S.W., Grundmann, H.J. et al. Synergy of simultaneous administration of ofloxacin and granulocyte colony-stimulating factor in killing ofEscherichia coli by human neutrophils. Infection 23, 298–300 (1995). https://doi.org/10.1007/BF01716290
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DOI: https://doi.org/10.1007/BF01716290