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A single-bead decode strategy using electrospray ionization mass spectrometry and a new photolabile linker: 3-Amino-3-(2-nitrophenyl)propionic acid

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Summary

A new linker that employs a photosensitive 3-ammo-3-(2-nitrophenyl)propionyl functionality (ANP-resin) has been developed for the preparation of C-terminal carboxamides. A wide range of carboxamides were prepared and identified using the ANP-resin and electrospray ionization mass spectrometry. A single bead containing tripeptide Fmoc-Asp-Arg(Tos)-Val-NH2 was isolated, photocleaved and the peptide was characterized by tandem mass spectrometry, thereby verifying a library decode strategy that avoids complex tagging procedures.

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  1. Diversity Sciences Department, Glaxo Wellcome Inc., 5 Moore Drive, 27709, Research Triangle Park, NC, USA

    Bradley B. Brown, David S. Wagner & H. Mario Geysen

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  2. David S. Wagner
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Brown, B.B., Wagner, D.S. & Geysen, H.M. A single-bead decode strategy using electrospray ionization mass spectrometry and a new photolabile linker: 3-Amino-3-(2-nitrophenyl)propionic acid. Mol Divers 1, 4–12 (1995). https://doi.org/10.1007/BF01715804

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