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Rapid screening for bacteriuria in pregnancy

Rascher Nachweis der Bakteriurie bei Schwangeren

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Summary

We evaluated a bioluminescence assay as a screening test for the detection of bacteriuria in pregnancy. A total of 1,000 urine specimens from a randomly selected group of pregnant women undergoing prenatal surveillance was investigated. Sequential dilution of urine specimens on CLED agar plates served as a reference method. Set against the reference group, bioluminescence screening scored a 93% sensitivity, a 78% specificity and a 99% predictive accuracy for negative results. All urine specimens were also analysed chemically for the presence of nitrite and leucocyte esterase by dip sticks. Dip sticks proved to be insufficient because of poor sensitivities of 54% and 59%, respectively. The bioluminescence assay is an effective, time- as well as labor-saving but questionably cost-effective method for the detection of bacteriuria in pregnancy.

Zusammenfassung

In der vorliegenden Studie prüften wir die klinische Wertigkeit eines Biolumineszenz-Verfahrens zum Nachweis der Bakteriurie bei Schwangeren. Insgesamt wurden 1000 Routine-Harnproben von Schwangeren untersucht. Als Referenzmethode diente die sequentielle Verdünnung der Harnproben und Ausstreichen auf CLED-Agarplatten. Eine signifikante Bakteriurie konnte mit der Biolumineszenz-Methode mit einer Sensitivität von 93% und einer Spezifität von 78% erkannt werden. Die Voraussagewahrscheinlichkeit für negative Resultate war 99%. Gleichzeitig untersuchten wir alle Harnproben auf Vorhandensein von Nitrit und Leukozyten-Esterase mittels Teststreifen. Die Sensitivitäten lagen nur bei 54% bzw. 59%. Mit Hilfe der Biolumineszenz-Methode kann eine signifikante Bakteriurie bei Schwangeren rasch, verläßlich, aber nur bei relevanter Probenanzahl auch kostengünstig nachgewiesen werden.

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Authors and Affiliations

  1. Universitätsklinik für Chemotherapie, Lazarettgasse 14, A-1090, Wien, Austria

    W. Graninger,  D. Fleischmann &  Laleh Aram

  2. I. Medizinische Universitätsklinik, Lazarettgasse 14, A-1090, Wien, Austria

    B. Schneeweiβ &  F. Stockenhauber

Authors
  1. W. Graninger
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  2. D. Fleischmann
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  3. Laleh Aram
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  4. B. Schneeweiβ
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  5. F. Stockenhauber
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Graninger, W., Fleischmann, D., Aram, L. et al. Rapid screening for bacteriuria in pregnancy. Infection 20, 9–11 (1992). https://doi.org/10.1007/BF01704885

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  • DOI: https://doi.org/10.1007/BF01704885

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