Abstract
Rice tungro spherical virus (RTSV) was shown to have three coat protein (CP) species by high resolution NaDodSO4-PAGE and Western blot analyses. The sequence of a coat protein-expressing cDNA clone that was identified and selected from a RTSV cDNA library showed that the insert was composed of 2823 bp with only one large open reading frame (ORF) coding for 941 amino acids. The positions of the three coat proteins were located in the putative polyprotein by N-terminal microsequencing and were shown to start at amino acids 287, 495, and 698 for CP-1, CP-2, and CP-3, respectively. The coat proteins are expressed as a polyprotein at the 5′ region of the viral RNA genome, and all are cleaved at glutamine carboxy termini, presumably by picornavirus 3C-type of protease(s). Sequence comparisons of coat proteins revealed that there are high amino acid homologies between CP-2 of RTSV and VP3s of encephalomyocarditis virus (EMCV) and Theiler's murine encephalomyelitis virus (TMEV). These results indicate that RTSV is a plant picornavirus.
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References
Hibino H., Ann Phytopathol Soc Jpn49 545–553, (1983).
Hibino H., Daguioag R.D., Mesina E.M., and Aguiero V.M., Plant Dis74 923–926, 1990.
Dahal G., Hibino H., Cabunagan R.C., Tiongco E.M., Flores Z.M., and Aguiero V.M., Phytopathology80 659–665, 1990.
Shimamoto K., Teda R., Izawa T., and Fujimoto H.,Nature 338 274–277, 1989.
Beachy R.N., Loesch-Fries S., and Tumer N.E., Annu Rev Phytopathol28 451–474, 1990.
Jones M.C., Gough K., Dasgupta I., Subba Rao B.L., Cliffe J., Qu R., Shen P., Kaniewska M., Blakebrough M., Davies J.W., Beachy R.N., and Hull R., J Gen Virol72 757–761, 1991.
Gerard G.F., Focus10 12–13, 1988.
Chaconas G. and van de Sande J.H. in Colowick S.P. and Kaplan N.O. (ed).Methods in Enzymology. Academic Press, New York, 1980 pp. 75–85.
Laemmli U.K., Nature227 680–685, 1970.
Blake, M.C., Johnston K.H., Russell-Jones G.J., and Gotschlich E.C., Anal Biochem136 175–179, 1984.
Sanger F., Nicklen S., and Coulson A.R., Proc Natl Acad Sci USA74 5463–5467, 1977.
Schagger H. and Jagow von G., Anal Biochem166 368–379, 1987.
Devereux J., Haeberli P., and Smithies O., Nucleic Acids Res12 387–395, 1984.
Palmenberg A.C., Kirby E.M., Janda M.R., Drake N.L., Duke G.M., Potratz K.F., and Collett M.S., Nucleic Acids Res12 2969–2985, 1984.
Pevear D.C., Calenoff M., Rozhou E., and Lipton H.L., J Virol61 1507–1516, 1987.
Hibino H., Ishikawa K., Omura T., Cabauatan P.Q., and Koganezawa H., Phytopathology81 1130–1132, 1991.
Hibino H., Adv Dis Vector Res6 209–241, 1991.
Davies J.W. and Hull R., J Gen Virol61 1–14, 1982.
Van Wezenbeek P., Verver J., Harmsen J., Vos P., and van Kammen A., EMBO J2 941–946, 1983.
Palmenberg A.C., Annu Rev Microbiol44 603–623, 1990.
Wellink J. and van Kammen A., Arch Virol98 1–26, 1988.
Krausslich H-G. and Wimmer E., Annu Rev Biochem57 701–754, 1988.
Dessens J.T. and Lomonossoff G.P., Virology184 738–746, 1991.
Rossmann M.G. and Johnson J.E., Annu Rev Biochem58 533–573, 1989.
Lomonossoff G.P. and Johnson J.E., Prog Biophys Mol Biol55 107–137, 1991.
Liljas L., Prog Biophys Mol Biol48 1–36, 1986.
Gingery R.E. in Koenig R. (ed).The Plant Viruses. Plenum Press, New York, 1988, pp 259–272.
Goldbach R., Microbiol Sci4 197–202, 1987.
Koonin E.V., Virus Genes5 273–282, 1991.
Argos P., Krammer G., Nicklin M.J.H., and Wimmer E., Nucleic Acids Res12 7251–7267, 1984.
Goldbach R., Annu Rev Phytopathol24 289–310, 1986.
King A.M.Q., Lomonossoff G.P., and Ryan M.D., Semin Virol2 11–17, 1991.
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Zhang, S., Jones, M.C., Barker, P. et al. Molecular cloning and sequencing of coat protein-encoding cDNA of rice tungro spherical virus—A plant picornavirus. Virus Genes 7, 121–132 (1993). https://doi.org/10.1007/BF01702392
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DOI: https://doi.org/10.1007/BF01702392