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Phospholipid fatty acid ester composition ofPasteurella multocida andActinobacillus lignieresii

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Abstract

Cell surface hydrophobicity properties vary dramatically, whereas cell envelope phospholipid composition is essentially identical among strains ofPasteurella multocida andActinobacillus lignieresii. Fatty acid ester composition of the major phospholipid fractions from cell surface hydrophobicity variants was examined to determine whether hydrophobic properties are influenced by cell envelope fatty acid content. Individual phospholipids were resolved by preparative thin-layer chromatography, and methanolysis was performed with boron trifluoride-methanol. Gas-liquid chromatographic analysis revealed the organisms to be similar qualitatively, whereas hydrophobic variants exhibited consistently, greater and more disparate C16:0+C16:1/C14:0 ratios in all fractions. Fatty acid composition of phospholipids may be related to surface hydrophobicity properties ofP. multocida variants. However, comparative data obtained forA. lignieresii revealed a degree of similarity withP. multocida that precludes use of this parameter as a means for differentiation of thesePasteurellaceae type species, thereby supporting their taxonomic relatedness.

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  1. Cynthia A. Fuller

    Present address: Department of Pulmonary Medicine, Louisiana State University Medical Center, P.O. Box 33932, 71130, Shreveport, LA, USA

Authors and Affiliations

  1. Department of Biological Sciences, Mississippi State University, P.O. Drawer GY, 39762, Mississippi State, Mississippi, USA

    Cynthia A. Fuller &  Franklin R. Champlin

  2. Mississippi State Chemical Laboratory, Mississippi State University, Mississippi State, Mississippi, USA

    Paul J. Brignac

Authors
  1. Cynthia A. Fuller
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  2. Paul J. Brignac
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  3. Franklin R. Champlin
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Fuller, C.A., Brignac, P.J. & Champlin, F.R. Phospholipid fatty acid ester composition ofPasteurella multocida andActinobacillus lignieresii . Current Microbiology 27, 237–240 (1993). https://doi.org/10.1007/BF01692882

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  • DOI: https://doi.org/10.1007/BF01692882

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