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Plant regeneration from protoplasts of cytoplasmic male sterile lines of rice (Oryza sativa L.)

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Abstract

This study compared plant regeneration from protoplasts isolated from suspension cultures of threeJaponica rice (Oryza sativa L.) lines with different male sterile cytoplasms. More than 180 green plants were regenerated from protoplasts from 5–8 month old suspensions of IR58024A, a line with the WA type of cytoplasmic male sterility (CMS). About 40% of the calli recovered from protoplasts produced green plants. ShuangbaiA (BT type of CMS) and Tai2A (Dian I type of CMS), both from Zhejiang province of China, responded less well in culture. ShuangbaiA produced green plants from 6.6% of calli, although initial protoplast yield per gram fresh weight was higher than for IR58024A. Tai2A showed lower protoplast yield, and only 1.1% of the calli produced green plants. Flow cytometric analyses of nuclear DNA content indicated that many of the regenerated plants were tetraploid. The percentage of tetraploids varied in the different lines. The male sterile characteristics of the original lines were maintained in the regenerated plants. Pollen abortion occured earliest in IR58024A and latest in Tai2A. IR58024A is a promising rice genotype for use as a recipient in direct gene transfer experiments.

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Abbreviations

BAP:

6-benzylaminopurine

CMS:

cytoplasmic male sterility

2,4-D:

2,4-dichlorophenoxyacetic acid

IRRI:

International Rice Research Institute

LS:

Linsmaier and Skoog's (1965) medium

MS:

Murashige and Skoog's (1962) medium

NAA:

α-naphthaleneacetic acid

WA:

wild abortive

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Author notes

  1. Qingzhong Xue

    Present address: Department of Agronomy, Zhejiang Agricultural University, 310029, Hangzhou, China

Authors and Affiliations

  1. Department of Plant Breeding and Biometry, Cornell University, 14853-1902, Ithaca, NY, USA

    Qingzhong Xue & Elizabeth D. Earle

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  1. Qingzhong Xue
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  2. Elizabeth D. Earle
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Communicated by J. Widholm

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Xue, Q., Earle, E.D. Plant regeneration from protoplasts of cytoplasmic male sterile lines of rice (Oryza sativa L.). Plant Cell Reports 15, 76–81 (1995). https://doi.org/10.1007/BF01690258

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  • DOI: https://doi.org/10.1007/BF01690258

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