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Soil adenosine triphosphate: Extraction, recovery and half-life

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Conclusions

Extraction of ATP from microorganisms in desert soil is best carried out using the n-butanol-octanol method. Using this method, one obtains an average ATP-recovery of 68% under widely varying soil moisture and textural conditions. We have also shown that the half-life of non-microbial ATP in soil is not more than one hour and thus the possibility of a build-up of non-microbial ATP in desert soil is unlikely. This method can be used for rapid survey of the microbial content in soil environments with a minimum of soil disruption. It is also a valuable tool for investigating the effect of various environmental factors on fluctuations in soil microflora content.

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Authors and Affiliations

  1. Department of Agricultural Chemistry and Soils, University of Arizona, 85721, Tucson, Ariz.

    A. R. Conklin Jr. & A. N. Macgregor

Authors
  1. A. R. Conklin Jr.
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  2. A. N. Macgregor
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This work was supported in part by funds allocated from the Desert Biome project NSF grant GB-15886 and from Regional Research Project W-111, University of Arizona Experiment Station.

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Conklin, A.R., Macgregor, A.N. Soil adenosine triphosphate: Extraction, recovery and half-life. Bull. Environ. Contam. Toxicol. 7, 296–300 (1972). https://doi.org/10.1007/BF01684528

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