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Mitochondrial DNA polymorphism and phylogenetic relationships inHevea brasiliensis

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Abstract

Using fourteen random mitochondrial DNA probes, we have examined restriction fragment length polymorphism (RFLP) in wild and cultivatedHevea brasiliensis. A total of 395 accessions, including 345 from various prospectings collected in Brazil, Colombia and Peru and 50 cultivated clones, were analyzed. Two other species (H. benthamiana andH. pauciflora) were also included in the study for comparison. The high level of mitochondrial polymorphism allowed us to divide all the accessions analyzed into 212 distinct genotypes. The genetic variability of cultivated clones was limited to four genotypes forming two clusters. In contrast, considerable genetic variation was found in the wild collections. In almost all cases, accessions displaying the same RFLP profile were restricted to the same geographical area (same or neighbor administrative districts). In addition, accessions whose genetic closeness was predicted by RFLP profiles were also clustered according to geographical origin. In a few cases, however, similar RFLP profiles were found for accessions originating from geographically distant districts. This discrepancy can be explained either by seed dispersion (by river) or possibly by similar genetic events occurring independently in different geographical locations. Chloroplast DNA RFLP was also analyzed in 217 accessions, representative of 126 distinct mitochondrial genotypes. Very few differences were found, indicating that the chloroplast genome is more highly conserved than the mitochondrial genome.

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References

  1. Allen PW: Fresh germplasm for natural rubber. Span 27: 7–8 (1984).

    Google Scholar 

  2. Apuya NR, Frazier BL, Kein P, Jill Roth E, Lark KG: Restriction fragment length polymorphisms as genetic markers in soybean,Glycine max (L.) Mervill. Theor Appl Genet 75: 889–901 (1988).

    Google Scholar 

  3. Benzécri JP: L'analyse des correspondances. In: L'analyse de Données, Tome 2, p. 616. Dunod, Paris (1973).

    Google Scholar 

  4. Besse P, Seguin M, Lebrun P, Chevallier MH, Nicolas D, Lanaud C: Genetic diversity among wild and cultivated populations ofHevea brasiliensis assessed by nuclear RFLP analysis. Theor Appl Genet 88: 199–207 (1994).

    Google Scholar 

  5. Boutry M, Briquet M: Mitochondrial modifications associated with the cytoplasmic male sterility in faba beans. Eur J Biochem 127: 129–135 (1982).

    PubMed  Google Scholar 

  6. Chevallier MH: Genetic variability ofHevea brasiliensis germplasm using isozyme markers. J Nat Rubb Res 3: 42–53 (1988).

    Google Scholar 

  7. Clegg MT: Preface to plant molecular evolution. Am Nat 130: S1-S5 (1987).

    Google Scholar 

  8. Clement-Demange A: IRCA Rubber Genetic Improvement Programme: first uses of new Amazonian origins. Goa, IRRDB Plant Breeders Meeting (1987).

    Google Scholar 

  9. Debener T, Salamini F, Gebhardt C: Phylogeny of wild and cultivatedSolanum species based on nuclear restriction fragment length polymorphisms (RFLPs). Theor Appl Genet 79: 360–368 (1990).

    Google Scholar 

  10. Gawel NJ, Jarret RL, Whittemore AP: Restriction fragment length polymorphism (RFLP)-based phylogenetic analysis ofMusa. Theor Appl Genet 84: 286–290 (1992).

    Google Scholar 

  11. Helentjaris T, King G, Slocum M, Siedenstrang C, Wegman S: Restriction fragment polymorphisms as probes for plant diversity and their development as tools for applied plant breeding. Plant Mol Biol 5: 109–118 (1985).

    Google Scholar 

  12. Hosaka K, Spooner DM: RFLP analysis of the wild potato species,Solanum acaule Bitter (Solanum sect.Petota). Theor Appl Genet 84: 851–858 (1992).

    Google Scholar 

  13. Jarret RL, Gawel NJ, Whittemore AP, Sharrocks S: RFLP-based phylogeny ofMusa species in Papua New Guinea. Theor Appl Genet 84: 579–584 (1992).

    Google Scholar 

  14. Jena KK, Kochert G: Restriction fragment length polymorphism analysis of CCDD genome species of the genusOryza L. Plant Mol Biol 16: 831–839 (1991).

    PubMed  Google Scholar 

  15. Keim P, Shoemaker RC, Palmer RG: Restriction fragment length polymorphism diversity in soybean. Theor Appl Genet 77: 786–792 (1989).

    Google Scholar 

  16. Kochert G, Halward T, Branch WD, Simpson CE: RFLP variability in peanut (Arachis hypogaea L.) cultivars and wild species. Theor Appl Genet 81: 565–570 (1991).

    Google Scholar 

  17. Laurent V, Risterucci C, Lanaud C: Chloroplast and mitochondrial DNA diversity inTheobroma cacao. Theor Appl Genet 87: 81–88 (1993).

    Google Scholar 

  18. Levings CS, Brown GG: Molecular biology of plant mitochondria. Cell 56: 171–179 (1989).

    PubMed  Google Scholar 

  19. Lonsdale DM: The plant mitochondrial genome. Biochem Plants 15: 229–295 (1989).

    Google Scholar 

  20. Miller JC, Tanksley SD: Effect of different restriction enzymes, probe source, and probe length on determining restriction fragment length polymorphism in tomato. Theor Appl Genet 80: 385–389 (1990).

    Google Scholar 

  21. Miller JC, Tanksley SD: RFLP analysis of phylogenetic relationship and genetic variation in the genusLycopersicon. Theor Appl Genet 80: 437–448 (1990).

    Google Scholar 

  22. Monte JV, McIntyre CL, Gustafson JP: Analysis of phylogenetic relationships in the Triticeae tribe using RFLPs. Theor Appl Genet 86: 649–655 (1993).

    Google Scholar 

  23. Nei M, Li WH: Mathematical model for studying genetic variation in terms of restriction endonuclease. Proc Nat Acad Sci USA 76: 5269–5273 (1979).

    PubMed  Google Scholar 

  24. Nei M: Molecular Evolutionary Genetics, pp. 106–107. Columbia University Press, New York (1987).

    Google Scholar 

  25. Newton KJ: Plant mitochondrial genomes: organization, expression and variation. Annu Rev Plant Physiol Mol Biol 39: 503–532 (1988).

    Google Scholar 

  26. Palmer JD: Chloroplast DNA evolution and biosystematic uses of chloroplast DNA variation. Am Nat 130: S6-S29 (1987).

    Google Scholar 

  27. Rogers SC, Bendich AY: Extraction of DNA from plant tissues. In: Gelvin SB, Schilperoort RA (eds) Plant Molecular Biology Manual, pp.Ab: 1–10. Kluwer Academic Publishers, Dordrecht, Netherlands (1988).

    Google Scholar 

  28. Saitou N, Nei M: The Neighbour-Joining method: a new method for reconstructing phylogenetic tree. Mol Biol Evol 4: 406–425 (1987).

    PubMed  Google Scholar 

  29. Saitou N, Imanishi T: Relative efficiencies of the Fitch-Margoliash, Maximum-parsimony, Maximum-likehood, Minimum-evolution, and Neighbour-Joining methods of phylogenetic tree construction in obtaining the correct tree. Mol Biol Evol 6: 514–525 (1989).

    Google Scholar 

  30. Scheepers D, Luo H, Boutry M: A variant RNA is associated with cytoplasmic male sterility inVicia faba. Arch Int Physiol Biochem 98: B93 (1990).

    Google Scholar 

  31. Schultes RE: WildHevea — an untapped source of germplasm. J Rubb Res Inst 54: 1–31 (1977).

    Google Scholar 

  32. Simmonds NW: Rubber breeding. In: Webster CC, Baullkwill WJ (eds) Rubber, pp. 85–124. Longman Singapore Publishers, Singapore (1989).

    Google Scholar 

  33. Small ID, Isaac PG, Leaver CJ: Stoichiometric differences in DNA molecules containing theatpA gene suggest mechanisms for the generation of mitochondrial genome diversity in maize. EMBO J 6: 865–869 (1987).

    Google Scholar 

  34. Sneath PHA, Sokal RR: Numerical Taxonomy. Freeman, San Francisco (1973).

    Google Scholar 

  35. Song KM, Osborn TC, William PH:Brassica taxonomy based on nuclear restriction fragment length polymorphism (RFLPs). 1. Genome evolution of diploid and amphidiploid species. Theor Appl Genet 75: 784–794 (1988).

    Google Scholar 

  36. Tao Y, Manners JM, Ludlow MM, Henzell RG: DNA polymorphism in grain sorghum (Sorghum bicolor (L.) Moench). Theor Appl Genet 86: 679–688 (1993).

    Google Scholar 

  37. Varghese YA: Germplasm resources and genetic improvements. In: Sethuraj MR, Mathew NM (eds) Developments in Crop Science 23. Natural Rubber Biology, Cultivation and Technology, pp. 89–115. Elsevier, Cambridge (1992).

    Google Scholar 

  38. Wang ZY, Tanksley SD: Restriction fragment length polymorphism inOryza sativa L. Genome 32: 1113–1118 (1989).

    Google Scholar 

  39. Wang ZY, Second G, Tanksley SD: Polymorphism and phylogenetic relationships among species in the genusOryza as determined by analysis of nuclear RFLPs. Theor Appl Genet 83: 565–581 (1992).

    Google Scholar 

  40. Wycherley PR: Rubber:Hevea brasiliensis (Euphorbiaceae). In: Simmonds NW (ed) Evolution of Crop Plants, pp. 77–81. Longman, London (1976).

    Google Scholar 

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Authors and Affiliations

  1. Unité de Biochimie Physiologique, Université Catholique de Louvain, Place Croix du Sud 2-20, B-1348, Louvain-la-Neuve, Belgium

    Hong Luo, Benoît Van Coppenolle & Marc Boutry

  2. CIRAD (Perennial Crops Department), BIOTROP, B.P. 5035, F-34032, Montpellier, France

    Marc Seguin

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  1. Hong Luo
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  2. Benoît Van Coppenolle
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  3. Marc Seguin
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  4. Marc Boutry
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Luo, H., Van Coppenolle, B., Seguin, M. et al. Mitochondrial DNA polymorphism and phylogenetic relationships inHevea brasiliensis . Mol Breeding 1, 51–63 (1995). https://doi.org/10.1007/BF01682089

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