Summary
Germination trials of pollen grains of the tea hybrid rose cultivars: ‘Ferry Porche’, ‘Bronze Masterpiece’, ‘Queen Elizabeth’, ‘John F. Kennedy’ and ‘Lady X’, were carried outin vitro, to determine whether these cultivars are suitable as pollen donors in a breeding programme.
Pollen grains of all five cultivars germinated poorly in a medium containing sucrose only. Addition of boric acid in the medium improved the germination percentages. The highest germinability was observed in ‘Ferry Porche’ and ‘Lady X’ on 15% sucrose+50 ppm boric acid, and 20% sucrose+100 ppm boric acid, respectively. Addition of calcium nitrate in the medium reduced germination percentages in all cultivars.
The effect of pH of the medium on pollen grain germinability depended on the cultivar and the composition of the medium. On a medium free of boric acid, the pH values between 5.5 and 7.0 caused a significant increase in the germination percentages of pollen grains of ‘Lady X’ and ‘Queen Elizabeth’. The addition of boric acid decreased the responsiveness of the pollen of all cultivars to the pH changes of the medium.
The germination capacity of pollen grains of ‘Lady X’ and ‘Ferry Porche’ was not affected by incubation temperatures ranging between 20°C and 30°C.
On the basis of the above results ‘Lady X’ and ‘Ferry Porche’ are considered the most suitable for pollen donors.
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References
Brewbacket, J.L. & B.H. Kwack, 1964. The calcium ion and substances influencing pollen growth. In: H.F. Lincens (Ed). Pollen Physiology and Fertilization. North Holland Publishing Company, pp. 143–151.
De Vries, D.P. & L. Dubois, 1987. The effect of temperature on fruit set, seed set and seed germination in ‘Sonia’ x ‘Hadley’ hybrid tea-rose crosses. Euphytica 36: 117–120.
Gudin, S., L. Arene & C. Pellegrino, 1991. Influence of temperature and hygrometry on rose pollen germination. Adv. Hort. Sci. 5: 96–98.
Kim, S.K., H.B. Lagerrstedt & L.S. Daley, 1985. Germination responses of filbert pollen to pH, temperature, glucose, fructose, and sucrose. HorScience 20: 944–946.
Kwack, B.H., 1965. The effect of calcium on pollen germination. Proc. Amer. Soc. Hort. Sci. 86: 818–823.
Luzny, J. & J. Burivalova, 1989. A contribution to the determination of pollen viability in selected garden rose and rootstock collection. Acta Universitatis Agriculturae. Facults Horticulturae, Brne. Chechoslovakia 4: 39–48. Hort. Abstr. 2339/1992.
Marchant, R., J.B. Power, M.R. Davey, J.M. Chartier-Hollis & P.T. Lynch, 1993. Cryopreservation of pollen from two rose cultivars. Euphytica 66: 235–241.
O'Kelley, J.C., 1955. External carbohydrates in growth and respiration of pollen tubesin vitro. Amer. J. Bot. 42: 322–327.
Pearson, H.M. & P.M. Harney, 1984. Pollen viability in Rosa. HortScience 19: 710–711.
Porlingis, I.C. & E.M. Sfakiotakis, 1968. Germinationin vitro and storage of olive pollen. Annals Agricultural and Forestry School. University of Thessaloniki, Vol. XII: 525–556.
Short, K.C. & A.V. Roberts, 1991.Rosa sp. (roses):in vitro culture, micropropagation and the production of secondary products. pp. 377–397. In: Y.P.S. Bajaj (Ed). Biotechnology in Agriculture and Forestry, Vol. III. Medicinal and Aromatic Plants. Springer-Verlag, Berlin.
Socias, R., D.E. Kester & M.V. Bradley, 1976. Effects of temperature and genotype on pollen tube growth in some self incompatible almond cultivars. J. Amer. Soc. Hort. Sci. 101: 490–493.
Therios, I.N., V.M. Tsirakoglou & K.N. Dimasi-Theriou, 1985. Physiological aspects of pistachio pollen germination. Riv. Ortoflorofrutt. It. 69: 161–170.
Thomson, M.M. & L.J. Liu, 1973. Temperature, fruit set and embryo sac development in ‘Italian’ prune. J. Amer. Soc. Hort. Sci. 98: 193–197.
Vasilakakis, M. & I.C. Porlingis, 1985. Effect of temperature on pollen germination pollen tube growth, effective pollination period, and fruit set of pear. HortScience 20:733–735.
Visser, T., 1955. Germination and storage of pollen Meded. Landbouwhogeschool Wageningen. Holland 55: 1–68.
Visser, T., D.P. De Vries, G.W.H. Welles & J.A.M. Scheurink, 1977. Hybrid Tea — rose pollen. I. Germination and storage. Euphytica 26: 721–728.
Yates, J.E. & D. Sparks, 1989. Hydration ad temperature influencein vitro gerrnination of pecan pollen. J. Amer. Soc. Hort. Sci. 114: 599–605.
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Voyiatzi, C.I. An assessment of thein vitro germination capacity of pollen grains of five tea hybrid rose cultivars. Euphytica 83, 199–204 (1995). https://doi.org/10.1007/BF01678130
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DOI: https://doi.org/10.1007/BF01678130