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Immunotherapy of a plasmacytoma with attenuated salmonella

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Abstract

An attenuated strain ofSalmonella typhimurium, SL3235, developed as a prototypic typhoid vaccine, is shown to retard growth of a murine plasmacytoma, TEPC-183, and to prolong survival of tumor-bearing mice. Live salmonella, but not acetone-killed organisms, had antitumor activity. The immunotherapeutic effect was demonstrable when the tumor was injected intralesionally or intraperitoneally. Increased survival, longer mean time to death, and retardation of tumor growth were found when the salmonella were given intralesionally as late as the sixth day post-tumor injection. Timing of salmonella inoculation, as well as the salmonella dose, had an effect on treatment efficacy. Injection of salmonella intraperitoneally exerted a strong antitumor effect when given as late as the third day post-tumor inoculation. The highest dose (2×106) of salmonella was less effective than doses 10- or 100-fold lower. TEPC-183 plasmacytoma is rapidly growing and highly immunosuppressive, so the ability of the salmonella to exert therapeutic activity against it is a measure of the potency of the vaccine. These observations are of interest, as they show that a genetically engineered, avirulent strain of Salmonella has immunotherapeutic properties similar to those of BCG and other biological response modifiers, and might have clinical potential as an antitumor agent.

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Author notes

  1. Bonnie Bushnell

    Present address: Merck, Sharp & Dohme, 19486, West Point, Pennsylvania, USA

  2. Niloofer Dalal

    Present address: Department of Anesthesiology, Temple University Hospital, 19140, Philadelphia, Pennsylvania, USA

  3. Rosana Schafer

    Present address: Department of Microbiology and Immunology, West Virginia University Sciences Center, 26506, Morgantown, West Virginia, USA

Authors and Affiliations

  1. Department of Microbiology and Immunology, Temple University School of Medicine, 3400 N. Broad St, 19140, Philadelphia, Pennsylvania, USA

    Toby K. Eisenstein, Bonnie Bushnell, Joseph J. Meissler, Niloofer Dalal, Rosana Schafer & H. Francis Havas

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  1. Toby K. Eisenstein
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  2. Bonnie Bushnell
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  5. Rosana Schafer
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  6. H. Francis Havas
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Eisenstein, T.K., Bushnell, B., Meissler, J.J. et al. Immunotherapy of a plasmacytoma with attenuated salmonella. Med Oncol 12, 103–108 (1995). https://doi.org/10.1007/BF01676710

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