Summary
The synthesis and secretion of von Willebrand factor (VWF, or Factor VIII-related antigen) and fibronectin by cultured endothelial cells from rhesus monkey choroid retina were demonstrated by immunofluorescence, immunoperoxidase and single radial immunodiffusion techniques. Both VWF and fibronectin are localized in intracellular granules and extracellular fibrils. The results of double immunofluorescence staining and post-embedding immunoelectron microscopy showed that there was a co-distribution of VWF and fibronectin not only in pericellular fibrils where they co-aligned with each other to be the components of extracellular matrix, but also in intracellular granules, suggesting they were synthesized or translocated in the same compartment.
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BAUGHMAN, S., HERRING, M., GLOVER, J. & DILLEY, R. (1984) The peroxidase antiperoxidase staining of Factor VIII-related antigen on cultured endothelial cells.J. Biomed. Mat. Res. 18, 561–6.
BIRDWELL, C. R., GOSPODÁROWICZ, D. & NICOLSON, G. L. (1978) Identification, localization and role of fibronectin in cultured bovine endothelial cells.Proc. Natl. Acad. Sci. USA 75, 3273–7.
CHAN, V. & CHAN, T. K. (1982) Characterization of Factor VIII related protein synthesized by human endothelial cell: A study of structure and function.Thromb. Haemostas. 48, 177–81.
FROMME, H. G., VOSS, B. PFAUTSCH, M., GROTE, M., VON FIGURA, K. & BEECK, H. (1982) Immunoelectronmicroscopic study on the location of fibronectin in human fibroblast cultures.J. Ultrastruct. Res. 80, 264–9.
FURCHT, L. T., SMITH, D., WENDELSCHAFER-CRABB, G., MOSHER, D. F. & FOIDART, J. M. (1980) Fibronectin presence in native collagen fibrils of human fibroblasts.J. Histochem. Cytochem. 28, 1319–33.
HEDMAN, K. (1980) Intracellular localization of fibronectin using immunoperoxidase cytochemistry in light and electron microscopy.J. Histochem. Cytochem. 28, 1233–41.
HORMIA, M. (1982) Expression of Factor VIII-related antigen and Ulex lectin binding sites in endothelial cells during long-term culture.Cell Bio. Int. Rep. 6, 1123–33.
HORMIA, M., LEHTO, V. P. & VIRTANEN, I. (1983) Factor VIII-related antigen. A pericellular matrix component of cultured human endothelial cells.Exp. Cell Res. 149, 483–97.
HORMIA, M., LEHTO, V. P. & VIRTANEN, I. (1984) Intracellular localization of Factor VIII-related antigen and fibronectin in cultured human endothelial cells: evidence for divergent routes of intracellular translocation.Eur. J. Cell Biol. 33, 217–28.
HOYER, L. W. (1981) The Factor-VIII complex: structure and function.Blood 58, 1–13.
HOYER, L. W., DE LOS SANTOS, R. P. & HOYER, J. R. (1973) Antihemophilic factor antigen, localization in endothelial cells by immunofluorescent microscopy.J. Clin. Invest. 52, 2737–44.
HYNES, R. O. & YAMADA, K. M. (1982) Fibronectins: multifunctional modular glycoproteins.J. Cell Biol. 95, 369–77.
IRISH, P. S. & HASTY, D. L. (1983) Immunocytochemical localization of fibronectin in human fibroblast cultures using a cell surface replica technique.J. Histochem. Cytochem. 31, 69–77.
JAFFE, E. A., HOYER, L. W. & NACKMAN, R. L. (1973) Synthesis of antihemophilic factor antigen by cultured human endothelial cells.J. Clin. Invest. 52, 2757–64.
JAFFE, E. A. MINICK, C. R., ADEHMAN, B. BECKER, C. G. & NACHMAN, R. (1976) Synthesis of basement membrane collagen by cultured human endothelial cells.J. Exp. Med. 144, 209–25.
JAFFE, E. A. & MOSHER, D. F. (1978) Synthesis of fibronectin by cultured human endothelial cellsJ. Exp. Med. 147, 1779–91.
JEANNEAU, C. & SULTAN, Y. (1982) Localization of Factor VIII/von Willebrand factor antigen by immunoelectron microscopy in human endothelial cells using Fab fragments coupled to peroxidase.J. Histochem. Cytochem. 30, 1091–6.
LAHAV, J. & HYNES, R. O. (1981) Involvement of fibronectin, von Willebrand factor and fibronectin in platelet interaction with solid substrata.J. Supramol. Struct. Cell Biochem. 17, 299–311.
LAWLER, J. W., SLAYTER, H. S. & COLIGAN, J. E. (1978) Isolation and characterization of a high molecular weight glycoprotein from human blood platelets.J. Biol. Chem. 253, 8609–16.
LOU, D. A. & HU, F. (1987) Specific antigen and organelle expression of long-term rhesus endothelial cell line.In Vitro,23, 75–92.
MACARAK, E. J., KIRBY, E., KIRK, T. & KEFALIDES, N. A. (1978) Synthesis of cold-soluble globulin by cultured calf endothelial cells.Proc. Natl. Acad. Sci. USA 75, 2621–5.
MADIN, J. A., DREYER, B., PITLICK, F. A. & FUTHMAYR, H. (1980) The collagenous components of the subendothelium — correlation of struction and function.Lab. Invest. 43, 303–15.
MOSHER, D. F., DOYLE, M. J. & JAFFE, E. A. (1982) Synthesis and secretion of thrombospondin and fibronectin by cultured human endothelial cells. InPathobiology of the Endothelial Cell (edited by NOSSEL, H. L. and VOGEL, H. J.) pp. 201–13. New York: Academic Press.
NEWMAN, G. R. & JASANI, B. (1984) Post-embedding immunoenzyme techniques. InImmunolabelling for Electron Microscopy (edited by POLAK, J. M. and VARNDELL, I. M.) pp. 53–70. New York: Elsevier Science Publishers.
NEWMAN, G. R., JASANI, B. & WILLIAMS, E. D. (1983) A simple post-embedding system for the rapid demonstration of tissue antigens under the electron microscope.Histochem. J. 15, 543–55.
PASZTOR, L. M. & HU, F. (1973) Chromosomal evolution in a rhesus cell line.Folia Primat. 19, 28–34.
PIOVELLA, F., NALLI, G., MALAMANI, G. D., MAJOLINO, I., FRASSONI, F. & SITAR, G. M. (1978) The ultrastructural localization of factor VIII-antigen in human platelet, megakaryocytes and endothelial cells utilizing a ferritin-labelled antibody.Brit. J. Haematol. 39, 209–13.
PLOW, E. F., BIRDWELL, C. & GINSBERG, M. H. (1979) Identification and quantitation of platelet associated fibronectin antigen.J. Clin. Invest. 63, 540–3.
SAGE, H., PRITZL, P. & BORNSTEIN, P. (1981) Secretory phenotypes of endothelial cells in culture: comparison of aortic, venous, capillary, and corneal endothelium.Arteriosclerosis 1, 427–42.
STENMAN, S., WARTIOVAARA, J. & VOHERI, A. (1977) Changes in the distribution of a major fibroblast protein, fibronectin, during mitosis and interphase.J. Cell Biol. 74, 453–67.
TUDDENHAM, E. G. D., LAZARCHICK, J. & HOYER, L. W. (1981) Synthesis and release of factor VIII by cultured human endothelial cells.Brit. J. Haematol. 47, 617–26.
WAGNER, D. D., OLMSTED, J. B. & MARDER, V. J. (1982) Immunolocalization of von Willebrand protein in Weibel-Palade bodies of human endothelial cells.J. Cell Biol. 95, 355–60.
YAMADA, S. S., YAMADA, K. M. & WILLINGHAM, M. C. (1980) Intracellular localization of fibronectin by immunoelectron microscopy.J. Histochem. Cytochem. 28, 953–60.
ZUCKER, M. B., MOSESSON, M. W., BROEKMAN, M. J. & KAPLAN, K. L. (1979) Release of platelet fibronectin (cold-soluble globulin) from alpha granules induced by thrombin or collagen; lack of requirement for plasma fibronectin in ADP-induced platelet aggregation.Blood 54, 8–12.
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Lou, DA., Hu, F. Co-distribution of von Willebrand factor and fibronectin in cultured rhesus endothelial cells. Histochem J 19, 431–438 (1987). https://doi.org/10.1007/BF01675754
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DOI: https://doi.org/10.1007/BF01675754