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Sequence analysis of the rDNA spacer ofParacentrotus lividus and observations about pre-rRNA processing

NTS sequence of Paracentrotus lividusrDNA

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Abstract

We have isolated and sequenced one intergenic region and a small part of the flanking regions (18S and 26S rRNA coding regions) of the rRNA-encoding genes (rDNA) from the sea urchinParacentrotus lividus. This region is about 3.8 Kb long. Northern blot hybridizations and S1 mapping experiments demonstrated the presence of a partially processed 21S rRNA precursor which has the same 5′ terminus as the 32S primary precursor, also in developmental stages characterized by a low rate of rRNA synthesis.

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Abbreviations

bp:

base pair(s)

Kb:

Kilobase(s) or 1000 bp

nt:

nucleotide(s)

rDNA:

DNA encoding rRNA

rRNA:

ribosomal RNA

S:

sedimentation constant

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Authors and Affiliations

  1. Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Palermo, Via Archirafi 22, Palermo, Italy

    M. Cantone & R. Barbieri

  2. Istituto di Biologia dello Sviluppo del C.N.R., Via Archirafi 20, 90123, Palermo, Italy

    G. Duro, V. Izzo & G. Giudice

Authors
  1. M. Cantone
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  2. R. Barbieri
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  3. G. Duro
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  4. V. Izzo
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  5. G. Giudice
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Cantone, M., Barbieri, R., Duro, G. et al. Sequence analysis of the rDNA spacer ofParacentrotus lividus and observations about pre-rRNA processing. Mol Biol Rep 18, 177–182 (1993). https://doi.org/10.1007/BF01674428

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  • DOI: https://doi.org/10.1007/BF01674428

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