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Measurement of cell proliferation and cell mediated contraction in 3-dimensional hydrated collagen matrices

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Journal of tissue culture methods

Summary

This report describes simplified methods for the preparation of reconstituted collagen gels containing a uniform distribution of anchorage dependent cells in 96-well microtitration plates, their dissolution with collagenase allow harvesting of either cells or medium, and objective analytical methods for measuring cell mediated contraction of collagen gels and [3 H]thymidine incorporation rates.

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Authors and Affiliations

  1. Ophthalmic Research Unit, University of Western Australia Department of Surgery, Royal Perth Hospital, 6000, Perth, Western Australia

    Francesco M. van Bockxmeer & Clayton E. Martin

Authors
  1. Francesco M. van Bockxmeer
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  2. Clayton E. Martin
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Additional information

To whom requests for reprints should be addressed at Opthalmic Research Unit, University of Western Australia, Royal Perth Hospital, GPO Box X2213, PERTh 6001, Western Australia.

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van Bockxmeer, F.M., Martin, C.E. Measurement of cell proliferation and cell mediated contraction in 3-dimensional hydrated collagen matrices. Journal of Tissue Culture Methods 7, 163–167 (1982). https://doi.org/10.1007/BF01672111

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  • DOI: https://doi.org/10.1007/BF01672111

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