Skip to main content
Log in

Studies on the ship-fouling algaEnteromorpha

III. Cytochemistry and autoradiography of adhesive production

  • Published:
Protoplasma Aims and scope Submit manuscript

Summary

A cytochemical technique for detection of polysaccharides indicates that the Golgi-derived adhesive vesicles in swimming zoospores contain glycoprotein. The carbohydrate moiety appears to be polymerized after the vesicles have become detached from the Golgi apparatus.

EM autoradiography usingL-leucine-4,5-3H shows that protein synthesized in the ER passes through the Golgi complex into the adhesive vesicles. On settlement the vesicles discharge their content as a fibrillar attaching material.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Baker, J. R. J., andL. V. Evans, 1973: The ship fouling algaEctocarpus. I. Ultrastructure and cytochemistry of plurilocular, reproductive stages. Protoplasma77, 1–13.

    Google Scholar 

  • Beams, H. W., andR. G. Kessel, 1968: The Golgi apparatus, structure and function. Int. Rev. Cytol.23, 209–276.

    Google Scholar 

  • Bonnett, H. T., andE. H. Newcomb, 1966: Coated vesicles and other cytoplasmic components of growing root hairs of radish. Protoplasma62, 59–75.

    Google Scholar 

  • Bonneville, M. A., andB. R. Voeller, 1963: A new cytoplasmic component of plant cells. J. Cell Biol.18, 703–708.

    Google Scholar 

  • Bråten, T., 1971: The ultrastructure of fertilization and zygote formation in the green algaUlva mutabilis Foyn. J. Cell Sci.9, 621–635.

    Google Scholar 

  • Brown, R. M., Jr.,W. W. Franke, H. Kleinig, H. Falk, andP. Sitte, 1970: Scale formation in chrysophycean algae. I. Cellulosic and non cellulosic wall components made by the Golgi apparatus. J. Cell Biol.45, 246–271.

    Google Scholar 

  • Caro, L. G., R. V. van Tubergen, andJ. A. Kolb, 1962: High resolution autoradiography I. Methods. J. Cell Biol.15, 173–188.

    Google Scholar 

  • Chamberlain, A. H. L., andL. V. Evans, 1973: Aspects of spore production in the red algaCeramium. Protoplasma76, 139–159.

    Google Scholar 

  • Christie, A. O., L. V. Evans, andM. Shaw, 1970: Studies on the ship-fouling algaEnteromorpha. II. The effect of certain enzymes on the adhesion of zoospores. Ann. Bot.34, 467–482.

    Google Scholar 

  • Dashek, W. V., andW. G. Rosen, 1966: Electron microscopical localization of chemical components in the growth zone of lily pollen tubes. Protoplasma61, 192–204.

    Google Scholar 

  • Evans, L. V., andA. O. Christie, 1970: Studies on the ship-fouling algaEnteromorpha. I. Aspects of the fine-structure and biochemistry of swimming and newly settled zoospores. Ann. Bot.34, 451–466.

    Google Scholar 

  • Gooday, G. W., 1971: A biochemical and autoradiographic study of the role of the Golgi bodies in thecal formation inPlatymonas tetrathele. J. Exp. Bot.22, 959–971.

    Google Scholar 

  • Green, J. C., andD. H. Jennings, 1967: A physical and chemical investigation of the scales produced by the Golgi apparatus within and found on the surface of the cells ofChrysochromulina chiton Parke et Manton. J. Exp. Bot.18, 359–370.

    Google Scholar 

  • Haddad, A., M. D. Smith, A. Herscovics, N. J. Nadler, andC. P. Leblond, 1971: Radioautographic study ofin vivo andin vitro incorporation of fucose-3H into thyroglobulin by rat thyroid follicular cells. J. Cell Biol.49, 856–882.

    Google Scholar 

  • Kwon, Y. M., andB. R. Grant, 1971: Assimilation and metabolism of glucose byDunaliella tertiolecta. I. Uptake by whole cells and metabolism by cell free systems. Plant and Cell Physiol.12, 29–39.

    Google Scholar 

  • Manton, I., 1967: Further observations on the fine structure ofChrysochromulina chiton with special reference to the haptonema, “peculiar” Golgi structure and scale production. J. Cell Sci.2, 265–272.

    Google Scholar 

  • Micalef, H., etP. Gayral, 1972: Quelques aspects de l'infrastructure des cellules végétatives et des cellules reproductrices d'Ulva lactuca L. (Chlorophycees). J. Microscopie13, 417–428.

    Google Scholar 

  • Morré, D. J., H. H. Mollenhauer, andC. E. Bracker, 1971: Origin and continuity of Golgi apparatus. In: Origin and continuity of cell organelles (J. Reinert andH. Ursprung, eds., pp. 82–126), Berlin-Heidelberg-New York: Springer.

    Google Scholar 

  • Northcote, D. H., 1971: The Golgi apparatus. Endeavour30, 26–33.

    Google Scholar 

  • Peters, T., andC. A. Ashley, 1967: An artefact in radioautography due to binding of free amino acids to tissues by fixatives. J. Cell Biol.33, 53–60.

    Google Scholar 

  • Pickett-Heaps, J. D., 1968: Further ultrastructural observations on polysaccharide localization in plant cells. J. Cell Sci.3, 55–64.

    Google Scholar 

  • Rambourg, A., W. Hernandez, andC. P. Leblond, 1969: Detection of complex carbohydrates in the Golgi apparatus of rat cells. J. Cell Biol.40, 395–414.

    Google Scholar 

  • Retallack, B., andR. D. Butler, 1972: Reproduction inBulbochaete hiloensis (Nordst.) Tiffany. I. Structure of the zoospore. Arch. Mikrobiol.86, 265–280.

    Google Scholar 

  • Rogers, A. W., 1967: Techniques of autoradiography. Amsterdam-London-New York: Elsevier.

    Google Scholar 

  • Schnepf, E., 1966: Feinbau und Funktion pflanzlicher Drüsen. Umschau. Wiss. Technik16, 522–527.

    Google Scholar 

  • —, 1969: Sekretion und Exkretion bei Pflanzen. Protoplasmatologia8, Wien-New York: Springer.

    Google Scholar 

  • Thiéry, J.-P., 1967: Mise en évidence des polysaccharides sur coupes fines en microscopie électronique. J. Microscopie6, 987–1017.

    Google Scholar 

  • —, 1969: Rôle de l'appareil de Golgi dans la synthèse des mucopolysaccharides. Etude cytochimique. I. Mise en évidence de mucopolysaccharides dans les vésicules de transition entre l'ergastoplasme et l'appareil de Golgi. J. Microscopie8, 689–708.

    Google Scholar 

  • Vogel, A., 1960: Zur Feinstruktur der Drüsen vonPinguicula. Beih. Z. schweiz. Forstverw.30, 113–122.

    Google Scholar 

  • Williams, M. A., 1969: The assessment of electron microscopic autoradiographs. In: Advances in Optical and Electron Microscopy3, 219–272.

    Google Scholar 

  • Woude, W. J. van der, D. J. Morré, andC. E. Bracker, 1971: Isolation and characterisation of secretory vesicles in germinated pollen ofLilium longiflorum. J. Cell Sci.8, 331–351.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Callow, M.E., Evans, L.V. Studies on the ship-fouling algaEnteromorpha . Protoplasma 80, 15–27 (1974). https://doi.org/10.1007/BF01666348

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01666348

Keywords

Navigation