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Methodology of long-term culture of human hemopoietic cells

  • Published:
Journal of tissue culture methods

Summary

Long-term culture (LTC) of hemopoietic cells has become a relatively specific operational term that is used to refer to a system in which very primitive hemopoietic cells can survive, proliferate, and differentiate into precursors of many lineages in the absence of exogenously added growth factors, but in the presence of other “stromal” cells. The latter are also of marrow origin but are developmentally unrelated to hemopoietic cells. Not surprisingly, therefore, a knowledge of the essential components of the system is required to initiate and maintain cultures in which sustained hemopoiesis will be reproducibly obtained. A considerable body of empirical data has been accumulated to define procedures that achieve this with normal human marrow, and analysis of the system itself has provided an understanding of some of the cellular and molecular dynamics that take place. This review surveys some of the more important features of LTC of human hemopoietic cells, provides associated methodologic information, and summarizes some current and anticipated applications.

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Authors and Affiliations

  1. Terry Fox Laboratory, B. C. Cancer Agency, 601 West 10th Avenue, V5Z 1L3, Vancouver, British Columbia, Canada

    Connie J. Eaves, Johanne D. Cashman & Allen C. Eaves

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  1. Connie J. Eaves
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  2. Johanne D. Cashman
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  3. Allen C. Eaves
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Eaves, C.J., Cashman, J.D. & Eaves, A.C. Methodology of long-term culture of human hemopoietic cells. Journal of Tissue Culture Methods 13, 55–61 (1991). https://doi.org/10.1007/BF01666132

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