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Preparation of primary monolayer cultures of postnatal rat liver cells

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Journal of tissue culture methods

Summary

We describe a perfusion with collagenase followed by dissociation with collagenase to isolate hepatocytes from 7 to 10-d postnatal rat liver. Subsequent culture in an arginine-deficient medium selects for hepatocytes by preventing overgrowth by other cell types that are unable to synthesize arginine.

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References

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Authors and Affiliations

  1. Department of Pharmacology and Drug Dynamics Institute College of Pharmacy, The University of Texas, 78712, Austin, Texas, USA

    Daniel Acosta, David C. Anuforo & Robert V. Smith

Authors
  1. Daniel Acosta
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  2. David C. Anuforo
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  3. Robert V. Smith
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Acosta, D., Anuforo, D.C. & Smith, R.V. Preparation of primary monolayer cultures of postnatal rat liver cells. Journal of Tissue Culture Methods 6, 35–37 (1980). https://doi.org/10.1007/BF01665904

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  • DOI: https://doi.org/10.1007/BF01665904

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