Summary
On the basis of the following evidence, it has been concluded that extracts ofBacillus megaterium KM contain a B12 coenzyme-dependent ribonucleotide reductase. The reduction of cytidine nucleotides to deoxycytidine nucleotides is enhanced by the addition of B12 coenzyme. In addition, the presence of hydroxyurea, a specific inhibitor of non-B12 coenzymedependent reductases, has only a slight effect on this reduction. Finally,B. megaterium extracts catalyze a transfer of tritium from 5′-deoxyadenosylcobalamin-5′-3H2 to water, a reaction specific for B12 coenzyme-dependent ribonucleotide reductases.
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Yau, S., Wachsman, J.T. The Bacillus megaterium ribonucleotide reductase: Evidence for a B12 coenzyme requirement. Mol Cell Biochem 1, 101–105 (1973). https://doi.org/10.1007/BF01659943
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DOI: https://doi.org/10.1007/BF01659943