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The significance of portal vein chemotherapy for liver micrometastases: An experimental study of a rat model

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Abstract

To clarify the significance of prophylactic portal vein chemotherapy for hepatic metastases, the correlation between the timing of the portal infusion and the growth of liver micrometastases was examined in a rat model. Male Donryu rats weighing 160-180g were first inoculated intraportally with 5 X 106 ascites hepatoma AH60C cells, following which an intraportal infusion of 5-fluorouracil (5-FU) 20 mg/kg/day with heparin 100 U/kg/day was given over 5 days, commencing on day 0, 3, and 6, in groups A, B, and C, respectively. Compared with a control group of rats which received no treatment, a significant inhibition of tumor growth and prolongation of survival time were observed in groups A (P < 0.01) and B (P < 0.05); however, group C, in which the mean diameter of the micrometastases was 0.52 ±0.10 mm at the commencement of the portal infusion, showed no therapeutic response. These results suggest that prophylactic portal vein chemotherapy should be given to prevent the lodgement of tumor cells in the portal system and inhibit their initial proliferation, rather than to destroy established micrometastases.

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References

  1. Gastrointestinal Tumor Study Group (1984) Adjuvant therapy of colon cancer: results of a prospectively randomized trial. New Engl J Med 310:737–743

    Google Scholar 

  2. Metzger U, Mermillod MS, Aeberhard P, Gloor F, Bissat A, Egeli R, Laffer U, Martinoli S, Mueller W, Schroeder R, Weber W, Swiss Group for Clinical Cancer Research (SAKK) (1987) Intraportal chemotherapy in colorectal carcinoma as an adjuvant modality. World J Surg 11:452–458

    Google Scholar 

  3. Wereldsma JCJ, Bruggink EDM, Meijer WS, Roukema JA, van Putten WL (1990) Adjuvant portal liver perfusion in colorectal cancer with 5-fluorouracil/heparin versus urokinase versus control. Cancer 65:425–432

    Google Scholar 

  4. Molzahn E, Gruenagel HH, Freud U, Gross D (1988) Konzepte zur Prophylaxe und Behandlung von Lebermetastasen beim colorectalen Carcinom durch regionale Chemotherapie. Chirurg 59:34–40

    Google Scholar 

  5. Wolmark N, Rockette H, Wickerham DL, Fisher B, Redmond C, Fisher ER, Potvin M, Davies RJ, Jones J, Robidoux A, Wexler M, Gordon P, Cruz AB Jr, Horsley S, Nims TA, Thirlwell M, Phillips WA, Prager D, Stern HS, Lerner HJ, Frazier TG (1990) Adjuvant therapy of Dukes' A, B, and C adenocarcinoma of the colon with portal-vein fluorouracil hepatic infusion: Preliminary results of National Surgical Adjuvant Breast and Bowel Project Protocol C-02. J Clinic Oncol 8:1466–1475

    Google Scholar 

  6. Beart RW Jr, Moertel CG, Wieand HS, Leigh JE, Windschid HE, van Heerden JA, Fitzgibbons RJ Jr, Wolff BG (1990) Adjuvant therapy for resectable colorectal carcinoma with fluorouracil administered by portal vein infusion. A study of the Mayo Clinic and the North Central Cancer Treatment Group. Arch Surg 125:897–901

    Google Scholar 

  7. Taylor I, Rowling JT, West C (1979) Adjuvant cytotoxic liver perfusion for colorectal cancer. Br J Surg 66:833–837

    Google Scholar 

  8. Une Y, Nagabuchi E, Ogasawara K, Kamiyama T, Saito M, Gotoda A, Sato N, Nakajima Y, Uchino J (1991) Intraportal vein infusion chemotherapy for hepatocellular carcinoma: Preliminary report (in Japanese with English abstract). Gan To Kagakuryouhou (Jpn J Cancer Chemother) 18:1195–1198

    Google Scholar 

  9. Tsujitani S, Watanabe A, Kakeji Y, Maehara Y, Tomoda H, Furusawa M, Sugimachi K (1991) Hepatic recurrence not prevented with low-dosage long-term intraportal 5-FU infusion after resection of colorectal liver metastasis. Eur J Surg Oncol 17:526–529

    Google Scholar 

  10. Finlay IG, McArdle CS (1986) Occult hepatic metastases in colorectal carcinoma. Br J Surg 73:732–735

    Google Scholar 

  11. Odashima S (1964) Establishment of ascites hepatoma in the rat, 1951–1962. Natl Cancer Inst Monogr 16:51–93

    Google Scholar 

  12. Ishida H, Iwama T, Imajo M, Mishima Y (1993) Establishment of continuous infusion system via hepatic artery or portal vein of the rat (in Japanese). Gan To Kagakuryouhou (Jpn J Cancer Chemother) 20:961–964

    Google Scholar 

  13. Tabuchi Y, Nakamura T, Imanishi K, Saito Y (1988) Usefulness of resorcin-fuchsin (Weigert) HE stain for various cancer specimens. Bull Allied Med Sci (Kobe) 4:89–93

    Google Scholar 

  14. Archer SG, Gray BN (1990) Comparison of portal vein chemotherapy with hepatic artery chemotherapy in the treatment of liver micrometastases. Am J Surg 159:325–329

    Google Scholar 

  15. Archer SG, Gray BN (1988) A reproducible model of hepatic and peritoneal metastases from colonic carcinoma. Eur J Cancer Clin Oncol 24:1623–1632

    Google Scholar 

  16. Ishida H, Iwama T, Nishioka Y, Ohkubo Y, Imajo M, Mishima Y (1992) An experimental study on portal vein chemotherapy as a prophylactic treatment against liver metastases (in Japanese with English abstract). Nippon Syoukakigeka Gakkai Zassi (Jpn J Gastroenterol Surg) 25:44–48

    Google Scholar 

  17. Tominaga T, Toshida Y, Kosaki G (1987) Liver metastases of colon 26 cells implanted into the superior mesenteric vein in mice. Jpn J Cancer Res (Gann) 78:846–850

    Google Scholar 

  18. Izumi B, Tashiro S, Miyauchi Y (1986) Anticancer effects of local administration of mitomycin C via the hepatic artery or the portal vein on implantation and growth of VXZ cancer injected into rabbit liver. Cancer Res 46:4167–4170

    Google Scholar 

  19. Ackerman NB, Lien WM, Kondi ES, Silverman NA (1969) The blood supply of experimental liver metastases. I. The distribution of hepatic artery and portal vein blood to “small” and “large” tumors. Surgery 66:1067–1072

    Google Scholar 

  20. Ackerman NB (1974) The blood supply of experimental liver metastases. IV. Changes in vascularity with increasing tumor growth. Surgery 75:589–596

    Google Scholar 

  21. Conway JG, Popp JA, Ji S, Thurman RG (1983) Effect of size on portal circulation of hepatic nodules from carcinogen-treated rats. Cancer Res 43:3374–3378

    Google Scholar 

  22. Fisher ER, Turnbull RB (1955) The cytologic demonstration and significance of tumor cells in the mesenteric venous blood in patients with colorectal carcinoma. Surg Gynecol Obstet 100:102–108

    Google Scholar 

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Authors and Affiliations

  1. Second Department of Surgery, Tokyo Medical and Dental University, 1-5-45 Yushima, 113, Bunkyo-ku, Tokyo, Japan

    Hideyuki Ishida, Takeo Iwama & Yoshio Mishima

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  1. Hideyuki Ishida
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  2. Takeo Iwama
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  3. Yoshio Mishima
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Ishida, H., Iwama, T. & Mishima, Y. The significance of portal vein chemotherapy for liver micrometastases: An experimental study of a rat model. Surg Today 24, 900–905 (1994). https://doi.org/10.1007/BF01651006

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  • DOI: https://doi.org/10.1007/BF01651006

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