Skip to main content
SpringerLink
Log in

Inhibition of electron-transport in chloroplasts by a quinone analogue: evidence for two sites of DPIPH2 oxidation

  • Published:
Journal of bioenergetics Aims and scope Submit manuscript

Abstract

A new inhibitor of photoreactions in chloroplasts, 2,3-dimethyl 5-dybroxy 6-phytol benzoquinone is shown to be an electron transfer inhibitor which blocks both cyclic and non-cyclic electron flow. Basal levels of electron transport from reduced dichlorophenol-indophenol to methyl viologen are not affected by the inhibitor, but uncoupler stimulated electron transport in the same system is inhibited. It is concluded that reduced dichlorophenol-indophenol can be oxidized by the photosynthetic electron transport chain in isolated chloroplasts at two sites: site I proximal to P700 and site II distal to P700. Site I has a low affinity for the electron donor. Electron flow from this site to methyl viologen does not suppert ATP formation and it is resistant to inhibition by the quinone analogue. Electron donation at site II, located on the linear portion of the electron transport chain between the two photosystems, has a higher affinity for reduced dichlorophenol-indophenol and precedes a phosphorylation site. The electron flow from this site is stimulated by uncouplers and inhibited by the quinone analogue.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

DPIP:

2,6-dichlorophenol indophenol

MeV:

methyl viologen

DCMU:

s-(s, t-dichlocophenyl-1,1-dimethylurca

CCP:

m-chlorocyanocarbonyl phenylthydrazone

DTE:

dithioerythritol

PMS:

phenaxine methosulfate

DMHPB:

2,3-dimethyl 5-hydroxy 6-phytol benzoquinone

References

  1. L. P. Vernon and M. Avron,Ann. Rev. Biochem.,34 (1965) 269.

    Google Scholar 

  2. H. Böhme and A. Trebst,Biochim. Biophys. Acta.,180 (1969) 137.

    Google Scholar 

  3. A. Trebet, H. Eck and S. Wagner, in:Photosynthetic. Mechanisms of Green Plants, B. Kok and A. T. Jagendorf (eds.), National Academy of Sciences, National Research Council. Washington, D.C., 1963, p. 174.

    Google Scholar 

  4. J. Neumann and Z. Drechsler,Plant Physiol.,42, (1967) 473.

    Google Scholar 

  5. F. L. Crane and M. D. Henninger,Vitams. Horm.,24, (1966) 489.

    Google Scholar 

  6. D. W. Krogmann and E. Olivero,J. Biol. Chem.,237 (1962) 3292.

    Google Scholar 

  7. D. I. ArnonPlant Physiol.,24 (1949) 1.

    Google Scholar 

  8. M. Avron,Biochim. Biophys. Acta.,40 (1960) 257.

    Google Scholar 

  9. L. P. Vernon and W. S. J. Zaugs,J. Biol. Chem.,235 (1960) 2728.

    Google Scholar 

  10. M. Losada, F. R. Whatley and D. I. Amon,Nature,190 (1961) 606.

    Google Scholar 

  11. M. Avron,Biochem. Biophys. Res. Comm.,17 (1964) 430.

    Google Scholar 

  12. A. Trebst and H. Eck,Z. Naturf,16b (1961) 455.

    Google Scholar 

  13. D. L. Keister,J. Biol. Chem.,238 (1963) PC2590.

    Google Scholar 

  14. D. L. Keister,J. Biol. Chem.,240 (1965) 2673

    Google Scholar 

  15. Z. Gromet-Elhanan,Biochim. Biophys. Acta.,131 (1967) 526.

    Google Scholar 

  16. B. Kok, in:Photosynthetic Mechanisms of Green Plants B. Kok and A. T. Jagendorf (eds.), National Academy of Sciences, National Research Council, Washington, D.C., 1963, p. 35.

    Google Scholar 

  17. L. W. Jones and B. Kok,Plant Physiol.,41 (1966) 1044.

    Google Scholar 

  18. N. I. Bishop, in:CIBA Foundation Symposium on Quinones in Electron Transport, G. E. W. Wolstenholme and C. M. O'Conner (eds.), Churchill Press, 1961, p. 383.

  19. T. Yamashita and W. L. Butler,Plant Physiol.,43 (1968) 2037.

    Google Scholar 

  20. S. Izawa, T. N. Connolly, G. D. Winget and N. E. Good, in:Energy Concersion by the Photosynthetic Apparatus, Brookhaven Symposia in Biology, No. 19, Upton, New York, 1967, p. 169.

  21. T. Yamashita and W. L. Butler,Plant Physiol.,43 (1968) 1978.

    Google Scholar 

  22. A. Trebst and E. Pistorius,Z. Naturf.,20B (1965) 143.

    Google Scholar 

  23. M. Schwartz,Biochim. Biophys. Acta,112 (1966) 204.

    Google Scholar 

  24. R. C. Honeycutt and D. W. Krogmann,Biochim. Biophys. Acta.,197 (1970) 267.

    Google Scholar 

Download references

Author information

Author notes

  1. C. J. Arntzen

    Present address: Department of Botany, University of Illinois, 61801, Urbana, Illinois

  2. J. Neumann

    Present address: Department of Botany, Tel-Aviv University, Tel-Aviv, Israel

Authors and Affiliations

  1. C. F. Kellering Research Laboratory, Yelloro Springs, Ohio

    C. J. Arntzen, J. Neumann & R. A. Dilley

Authors
  1. C. J. Arntzen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Neumann
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. A. Dilley
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Contribution No. 422 from the Charles F. Kettering Research Laboratory. This research supported in part by the National Science Foundation Grant No. G88432.

Supported by an NSF Post-doctoral Fellowship No. 49032.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Arntzen, C.J., Neumann, J. & Dilley, R.A. Inhibition of electron-transport in chloroplasts by a quinone analogue: evidence for two sites of DPIPH2 oxidation. J Bioenerg Biomembr 2, 73–83 (1971). https://doi.org/10.1007/BF01648922

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01648922

Keywords

Search

Navigation