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Sinusoidal endothelial liver cells in vitro release endothelin — Augmentation by transforming growth factorβ and Kupffer cell-conditioned media

Kultivierte sinusoidale Endothelzellen der Leber bilden und sezernieren Endothelin — Stimulation durch Transforming Growth Factorβ und konditionierte Kupfferzell Medien

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Summary

Endothelin is the most potent vasoconstrictor peptide known today. Using a radioimmunoassay for endothelin, we measured immunoreactive endothelin in culture media of guinea pig sinusoidal endothelial liver cells and human umbilical vein endothelial cells. A time-dependent release of immunoreactive endothelin by confluent sinusoidal endothelial liver cells in culture was found. Sinusoidal endothelial liver cells produced similar amounts of immunoreactive endothelin as umbilical vein endothelial cells, about 900 pg/μg DNA per 24 h. In the presence of transforming growth factorβ a dose-dependent increase of immunoreactive endothelin release was measured. The maximal increase of 50% was found at a concentration of 1 ng transforming growth factor per ml. To a similar extent Kupffer cell-conditioned media augmented the release of immunoreactive endothelin by sinusoidal endothelial liver cells, especially when Kupffer cells had been stimulated by endotoxin. Endotoxin itself did not alter the release of immunoreactive endothelin. Endothelin released by sinusoidal endothelial liver cells might influence the pericytes of the liver, i.e., the Ito-cells.

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Abbreviations

KC:

Kupffer cells

LSEC:

Liver sinusoidal endothelial cells

FCS:

fetal calf serum

RIA:

radioimmuno-assay

TGFβ :

transforming growth factorβ

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Authors and Affiliations

  1. I. Medizinische Klinik und Poliklinik, Johannes-Gutenberg Universität, Mainz

    H. Rieder, G. Ramadori & K. H. Meyer zum Büschenfelde

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  1. H. Rieder
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  2. G. Ramadori
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  3. K. H. Meyer zum Büschenfelde
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Dedicated to Professor Dr. Wolfgang Gerok on the occasion of his 65th birthday

Supported by Deutsche Forschungsgemeinschaft: grants Ri 460/1-2 and Ra 362/5-2

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Rieder, H., Ramadori, G. & Meyer zum Büschenfelde, K.H. Sinusoidal endothelial liver cells in vitro release endothelin — Augmentation by transforming growth factorβ and Kupffer cell-conditioned media. Klin Wochenschr 69, 387–391 (1991). https://doi.org/10.1007/BF01647411

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  • DOI: https://doi.org/10.1007/BF01647411

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