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Role of oxygen radicals in tourniquet-related ischemia-reperfusion injury of human patients

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Summary

In the current study we evaluated effluent blood from extremities of human patients undergoing reconstructive surgical treatment which is routinely accompanied by upper extremity exsanguination and application of a tourniquet. Following tourniquet release (reperfusion), there were immediate increases in the plasma levels of xanthine oxidase activity, uric acid, and histamine. Xanthine dehydrogenase activity was not detectable. Plasma also contained products consistent with the formation of oxygen-derived free radicals, namely hemoglobin and fluorescent compounds. Our data indicate in humans that ischemia-reperfusion events are associated with the appearance of xanthine oxidase activity and it's products in the plasma effluent.

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Abbreviations

XO:

xanthine oxidase

XD:

xanthine dehydrogenase

IPSI:

ipsilateral limb

CONTRA:

contralateral, limb

References

  1. Granger ND (1988) Role of xanthine oxidase and granulocytes in ischemia-reperfusion injury. Am J Physiol 255:H1269-H1275

    Google Scholar 

  2. Granger ND, Rutili G, McCord JM (1981) Superoxide radicals in feline intestinal ischemia. Gastroenterology 81:22–29

    Google Scholar 

  3. Petrone WF, English DK, Wong K, McCord JM (1980) Free radicals and inflammation: The superoxide-dependent activation of a neutrophil chemotactic tactor in plasma. Proc Natl Acad Sci USA 77:1159–1163

    Google Scholar 

  4. Grisham MB, Hernandez LA, Granger DN (1986) Xanthine oxidase and neutrophil infiltration in intestinal ischemia. Am J Physiol 251:G456-G574

    Google Scholar 

  5. Romson JL, Hook BG, Kunkel SL, Abrams GD, Schork MA, Lucchesi BR (1983) Reduction of the extent of ischemic myocardial injury by neutrophil depletion in dogs. Circulation 67:1016–1023

    Google Scholar 

  6. Hernandez LA, Grisham MB, Twohig B, Arfors KE, Harlan JM, Granger DN (1987) Role of neutrophils in ischemia-reperfusion induced microvascular injury. Am J Physiol 253:H699-H703

    Google Scholar 

  7. Eddy LJ, Stewart JR, Jones HP, Engerson TD, McCord JM, Downey JM (1987) Free radical-producing enzyme, xanthine oxidase, is undetectable in human hearts. Am J Physiol 253:H709-H711

    Google Scholar 

  8. Bailey IA, Blackwell CP, Woodward B (1988) Chemilumin-escence measurements of xanthine oxidase and xanthine dehydrogenase activity in four types of cardiovascular cell. Basic Res Cardiol 83:392–400

    Google Scholar 

  9. Jarasch ED, Bruder G, Heid HW (1986) Significance of xanthine oxidase in capillary endothelial cells. Acta Physiol Scand[Suppl] 548:39–46

    Google Scholar 

  10. Friedl HP, Till GO, Trentz O, Ward PA (1989) Roles of histamine, complement, and xanthine oxidase in thermal injury of skin. Am J Pathol 135:203–217

    Google Scholar 

  11. Ward PA, Till GO, Hatherill JR, Annesley TM, Kunkel R (1985) Systemic complement activation, lung injury, and products of lipid peroxidation. J Clin Invest 70:517–527

    Google Scholar 

  12. Waud WR, Rajagopalan KV (1976) The mechanism of conversion of conversion of rat liver xanthine dehydrogenase from NAD+-depdent form (type D) to an O2-dependent form (type 0). Arch Biochem Biophys 172:365–379

    Google Scholar 

  13. Hatherill JR, Till GO, Bruner LH, Ward PA (1986) Thermal injury, intravascular hemolysis, and toxic oxygen products. J Clin Invest 78:629–636

    Google Scholar 

  14. Dillard CJ, Tappel AL (1973) Fluorescent products have reaction of peroxidizing polyunsaturated fatty acids with phosphatide ethanolamine and phenylalanine. Lipids 8:183–189

    Google Scholar 

  15. Achterberg PW, Nieukoop AS, Schoutsen B, de Jong JW (1988) Different ATP-catabolism in reperfused adult and newborn rat hearts. Am J Physiol 254:H1091-H1098

    Google Scholar 

  16. Zweier JL, Kuppusamy P, Lutty GA (1988) Measurement of endothelial cell free radical formation: evidence for a central mechanism of free radical injury in postischemic tissues. Proc Natl Acad Sci USA 85:4046–4050

    Google Scholar 

  17. Weiss SJ (1982) Neutrophil-mediated methemoglobin formation in the erythrocyte: The role of superoxide anion and hydrogen peroxide. J Biol Chem 257:2947–2953

    Google Scholar 

  18. Fantone JC, Ward PA (1982) Role of oxygen-derived free radicals and metabolites in leukocyte-dependent inflammatory reactions. Am J Pathol 197:395–418

    Google Scholar 

  19. Pickering RJ, Wolfson MR, Good RA, Gewurz H (1969) Passive hemolysis by serum and cobra venom factor A new mechanism inducing membrane damage by complement. Proc Natl Acad Sci USA 62:521–527

    Google Scholar 

  20. Vogt W, von Zabern I, Hesse D, Nolte R, Haller Y (1987) Generation of an activated form of human C5 (C5b-like C5) by oxygen radicals. Immunol Lett 14:209–215

    Google Scholar 

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Authors and Affiliations

  1. Departement Chirurgie, Klinik für Unfallchirurgie, Universitätsspital Zürich, Schweiz

    H. P. Friedl & O. Trentz

  2. Department of Pathology, University of Michigan Medical School, Ann Arbor

    G. O. Till & P. A. Ward

Authors
  1. H. P. Friedl
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  2. G. O. Till
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  3. O. Trentz
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  4. P. A. Ward
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Additional information

Supported in part by grants GM-28499, GM 29507, HL 31963, GM 39397, HL-21568, and HL-33064 from the National Institutes of Health and FR 744/1 from the Deutsche Forschungsgemeinschaft

Manuscripts published in this issue were the matter of a Symposium held at the University of Ulm, April 24–27, 1991

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Friedl, H.P., Till, G.O., Trentz, O. et al. Role of oxygen radicals in tourniquet-related ischemia-reperfusion injury of human patients. Klin Wochenschr 69, 1109–1112 (1991). https://doi.org/10.1007/BF01645168

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  • DOI: https://doi.org/10.1007/BF01645168

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