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Factor VIII-related antigen in tissues detected by the indirect immunofluorescence technique

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Summary

A rabbit antibody was used to detect factor VIII-related antigen in tissue sections by means of the indirect immunofluorescence technique. Interaction between antibody and antigen was found on vascular intima associated with endothelial cells in all tissues studied. Since there is a close relationship between factor VIII-related antigen and biological factor VIII activity in normal plasma one may draw conclusions about the distribution of factor VIII in tissues.

Zusammenfassung

Ein Kaninchen-Antikörper wurde zur Darstellung des Faktor-VIII-assoziierten Antigens in Gewebeschnitten mit der indirekten Immunfluoreszenztechnik verwandt. Die Reaktionen zwischen Antikörper und dem Antigen wurden in allen untersuchten Körpergeweben auf der Intima von Gefäßen in Verbindung mit Endothelzellen gefunden. Da eine enge Beziehung zwischen Faktor-VIII-assoziiertem Antigen und biologischer Faktor-VIII-Aktivität in normalem Plasma besteht, kann man daraus möglicherweise Schlüsse über die Verteilung von Faktor VIII in Körpergeweben ziehen.

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Author notes

  1. Rainer Gruson

    Present address: I. Medizinische Abteilung, Marienkrankenhaus, Alfredstr. 9, 2000, Hamburg 76, BRD

Authors and Affiliations

  1. Oxford Haemophilia Centre, Churchill Hospital, Headington, Oxford

    Rainer Gruson & Charles R. Rizza

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  1. Rainer Gruson
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  2. Charles R. Rizza
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Gruson, R., Rizza, C.R. Factor VIII-related antigen in tissues detected by the indirect immunofluorescence technique. Blut 29, 241–250 (1974). https://doi.org/10.1007/BF01635545

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  • DOI: https://doi.org/10.1007/BF01635545

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