Skip to main content
SpringerLink
Log in

Über den Einfluß von Papain, Neuraminidase und AET auf das Verhalten von Lymphozyten im zytotoxischen Test

  • Published:
Blut: Zeitschrift für die Gesamte Blutforschung Aims and scope Submit manuscript

Zusammenfassung

Über die Wirkung von Papain, Neuraminidase und AET auf Lymphozyten wird berichtet. Alle drei Substanzen verstärken in niedrigen Konzentrationen oder kurzen Inkubationszeiten die lymphozytotoxische Reaktion, die bei höheren Konzentrationen oder längeren Inkubationszeiten abnimmt. Die mit diesen drei Substanzen verursachten Membranveränderungen sind unter definierten Konditionen reversibel.

Summary

The influence of papain, neuraminidase and AET to lymphocytes is reported. All three substances strengthen the lymphocytotoxic reaction in low concentrations or by short incubation time and lower it in higher concentrations or longer incubation time.

Membrane changes by these three substances are reversible under defined conditions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Literatur

  1. Bentwich Z., Douglas S. P., Skutelsky E. & Kunkel H. G.: Sheep red cell binding to human lymphocytes treated with Neuraminidase; enhancement of T cell binding hand identification of a subpopulation of B cells. Z. Exp. Med.137, 1532 (1973).

    Article  CAS  Google Scholar 

  2. Bekesi J. G., St.-Arneault G. & Holland J. F.: Increase of Leukemia L 1210 immunogenecity by Victoriaholerea neuraminidase treatment. Cancer Res.31, 2130 (1971).

    CAS  PubMed  Google Scholar 

  3. Bernoco D., Cullen S., Scudellen G., Trinchieri G. & Ceppelini R.: Hl-A molekules at the cell surface. Histokompatibility Testing 1972, 527.

  4. Bhandari S. C. & Singal D. P.: Removal and regeneration of Hl-A-antigens on peripheral blood lymphocytes. Tissue Antigens3, 140 (1973).

    Article  CAS  PubMed  Google Scholar 

  5. Böyum A.: Separation of leukocytes from blood and bone marrow. Scand. J. clin. Lab. Invest.21, 31 (1958).

    Google Scholar 

  6. Braun W. E., Grecek D. R. & Murphy J. J.: Expanded Hl-A phenotypes of human peripheral lymphocytes after trysinization. Transplantation13, 337 (1972).

    Article  CAS  PubMed  Google Scholar 

  7. Bube F. W., Siebel E. and Heumann H.: The possibility of influencing histokompatibility antigens by proteolytic enzymes. Vox Sang.25, 327 (1973).

    Article  CAS  PubMed  Google Scholar 

  8. Chapel H. H. & Welsh K. J.: Removal and resyneresis of Hl-A 2, 5 and 12 determinants at the surface of peripheral blood lymphocytes. Transplantation13, 347 (1972).

    Article  CAS  PubMed  Google Scholar 

  9. Cullen S. E., Bernoco D., Carbonara A. D., Jacot-Guillarmod H., Trinchieri G. & Ceppellini R.: Fate of Hl-A-antigens and antibodies at the lymphocyte surface. Transpl. Proc.5, 1835 (1973).

    CAS  Google Scholar 

  10. Currie G. A. & Bagshawe K. D.: The role of sialic acid in antigenic expression; further studies of the Landschütz ascides tumor. Brit. J. Cancer22, 843 (1968).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Currie G. A. & Bagshawe K. D.: Immunology at pregnancy: The foetomaternal barrier. Proc. roy. Soc. Med.61, 1206 (1968).

    CAS  PubMed  PubMed Central  Google Scholar 

  12. Currie G. A. & Bagshawe K. D.: Tumour specific immunogenecity of methylchloranthrene-induced sarcoma cells after incubation in neuraminidase Brit. J. Cancer23, 141 (1969).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Gibofski A. & Terasaki P. J.: Trypsinization of lymphocytes for HL-A typing. Transplantation13, 192 (1972).

    Article  Google Scholar 

  14. Grothaus E. A., Flye M. W., Yunis E. & Amos D. B.: Human lymphocytes antigen reactivity modified by neuraminidase. Science173, 542 (1971).

    Article  CAS  PubMed  Google Scholar 

  15. Kahan B. D. & Reisfeld R. A.: Transplantation Antigens. Science164, 514 (1969).

    Article  CAS  PubMed  Google Scholar 

  16. Kraemer P. M.: Regeneration of sialic acid on the surface of Chinese hamster cells in culture. J. Cell Physiol.68, 85 (1966).

    Article  CAS  PubMed  Google Scholar 

  17. Mann D. L., Fahey J. L. & Nathenson S. G.: Molecular comparisons of papain solubilized H-2 and HL-A alloantigens. Histokompatibility Testing 1970, 461.

  18. Mercuriali F., Richiardi P., Mattiuz P. L. & Sirchia G.: AET-treated lymphocytes; their use in the lymphocytotoxicity reaction. Tissue Antigens1, 290 (1971).

    Article  CAS  PubMed  Google Scholar 

  19. Mittal K. K., Mickey M. R., Singal D. P. & Terasaki P. J.: Serotyping for homotransplantation. XVIII. Refinement of microdroplet lymphocyte cytotoxicity test. Transplantation6, 911 (1968).

    Article  Google Scholar 

  20. Mittal K. K., Mickey M. R. & Terasaki P. J.: Serotyping for homotransplantation. XXXI. A 45-minute microcytotoxicity test. Transplantation8, 801 (1969).

    Article  CAS  Google Scholar 

  21. Pellegrino M. A., Ferrone S., DelVillano B. & Reisfeld R. A.: Effects of inhibitors of macromolecular synthesis on cell surface expression of HL-A antigens. Transplantation Proc.5, 91 (1973).

    CAS  Google Scholar 

  22. Ray P. K., Gewurz H. & Simmons R. L.: The mechanism of increased sensitivity of neuraminidase-treated cells to antibody induced cytolysis. Fed. Proc.29, 573 (1970).

    Google Scholar 

  23. Ray P. K., Gewurz H. & Simmons R. L.: The serological behaviour of neuraminidase-treated lymphoid cells. Clin. exp. Immunol.11, 441 (1971).

    Google Scholar 

  24. Reisner E. G. & D. B. Amos: The complement-binding and absorbation capacity of human white blood cells treated with neuraminidase. Transplantation14, 455 (1072).

    Article  Google Scholar 

  25. Reisner E. G., Flye M. W., Su Chung K. S. & Amos D. B.: The cytotoxic reactivity and sialic acid content of human lymphoid cells. Tissue Antigens4, 7 (1974).

    Article  CAS  PubMed  Google Scholar 

  26. Sachtleben P.: Neuraminidase and anti-Neuraminidase serum: Effect on the cell surface properties Vox. Sang25, 519 (1973).

    Article  CAS  Google Scholar 

  27. Schwartz B. D. & Nathenson S. G.: Regeneration of transplantation antigens on mouse cells. Transplantation Proc.3, 180 (1971).

    CAS  Google Scholar 

  28. Simmons R. L., Rios A. & Ray P. K.: Mechanism of neuraminidase induced antigen “unmasking”. Surg. Form.21, 265 (1971).

    Google Scholar 

  29. Simmons R. L., Rios A. & Ray P. K.: Immunogenity and antigenicity of lymphoid cells treated with neuraminidase. Nature (New Biol.)231, 179 (1971).

    Article  CAS  Google Scholar 

  30. Sirchia G. & Dacie J. V.: Immunolysis of AET-treated normal red cells (PNH-like cells). Nature215, 747 (1967).

    Article  CAS  PubMed  Google Scholar 

  31. Sirchia G., Scalamogna M., Pizzi G. & Mercuriali F.: Lymphocyte isolation and treatment with AET for routine use in the lymphocytoxicity test. Tissue Antigens3, 163 (1973).

    Google Scholar 

  32. Turner M. J., Strominger J. L. & Sanderson A. R.: Enzymic removal and re-expression of a histocompatibility antigen HL-A2, at the surface of human peripheral lymphocytes. Proc. Nat. Acad. Sci. (Wash.)69, 200 (1972).

    Article  CAS  Google Scholar 

  33. Yunis E. J., Ward F. E. & Amos D. B.: Observation of the CYNAP-phenomenon. Histocompatibility Testing 1970, 351.

  34. Miyajima et al.: Tissue Antigens2, 64 (1972).

    Article  CAS  PubMed  Google Scholar 

  35. Cannady et al.: Tissue Antigens4, 564 (1974).

    Article  Google Scholar 

Download references

Author information

Author notes

  1. Friedrich Wilhelm Bube,  Henning Heumann,  Dietmar Seegers &  Eberhard Siebel

    Present address: Abteilung für Transfusionsmedizin der Univ.-Kliniken Köln, Josef-Stelzmann-Str. 9, D-5, Köln 41

Authors and Affiliations

    Authors
    1. Friedrich Wilhelm Bube
      View author publications

      You can also search for this author in PubMed Google Scholar

    2. Henning Heumann
      View author publications

      You can also search for this author in PubMed Google Scholar

    3. Dietmar Seegers
      View author publications

      You can also search for this author in PubMed Google Scholar

    4. Eberhard Siebel
      View author publications

      You can also search for this author in PubMed Google Scholar

    Additional information

    2-Amino-isothiuroniumbromid, Fa. Serva, Heidelberg.

    Rights and permissions

    Reprints and permissions

    About this article

    Cite this article

    Bube, F.W., Heumann, H., Seegers, D. et al. Über den Einfluß von Papain, Neuraminidase und AET auf das Verhalten von Lymphozyten im zytotoxischen Test. Blut 31, 21–28 (1975). https://doi.org/10.1007/BF01635438

    Download citation

    • Received:

    • Published:

    • Issue Date:

    • DOI: https://doi.org/10.1007/BF01635438

    Search

    Navigation