Skip to main content
SpringerLink
Log in

Lymphozytenisolierung aus dem Blut des Menschen und der Tiere

  • Kurze Mitteilung
  • Published:
Blut Aims and scope Submit manuscript

Zusammenfassung

Nach Diskussion der verschiedenen Verfahren zur Gewinnung von Leukozyten und Lymphozyten wird eine Methode für die Reinisolierung von Lymphozyten aus dem Blut von Mensch, Pferd, Rind, Schaf, Schwein und Hund beschrieben. Die Methode beruht auf den unterschiedlichen spezifischen Gewichten der Blutzellen, wobei Jodamid und jodamidhaltige Präparate Verwendung finden. Bei den Untersuchungen haben sich besonders die jodamidhaltigen Röntgenkontrastmittel “Uromiro-300” und “Uromiro-380” bewährt.

Summary

After the discussion of the different methods for obtaining leucocytes and lymphocytes a method is described concerning the pure isolation of lymphocytes out of the blood of humans, horses, cattle, sheep, pigs and dogs. The method is based on the different specific weights of the blood cells. Jodamid and jodamid-compounds were used for this purpose. The X-ray-contrast media Uromiro-300 and Uromiro-380 containing both jodamid turned out to be especially effective.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Literatur

  1. Amos, D.: Cytotoxicity Testing, Histocompatibility Testing, Munksgaard, Kopenhagen 1966.

    Google Scholar 

  2. Astoldi, G.: Med. Klin.59, 368 (1964).

    Google Scholar 

  3. Behrens, M. und F. Esch: Experientia19, 406 (1963).

    Google Scholar 

  4. Binns, R. M., J. C. Hall, Brit. J. exp. Path.47, 275 (1966).

    Google Scholar 

  5. Bodmer, W.: Cytofluorchromasie. Manual of Tissue typing techniques. National Institute of Health, Berthesda, Maryland/USA 1969.

    Google Scholar 

  6. Böyum, A.: Nature [London]21, 793 (1964).

    Google Scholar 

  7. Böyum, A.: Scand. Clin. Lab. Invest.21, Suppl.97, 31 (1968).

    Google Scholar 

  8. Borel, J. F.: Inaug. Diss. Nr. 3485, ETH-Zürich 1964.

  9. Brendel, W. und R. Pichlmayr: Dtsch. Med. Wschr.92, 549 (1967).

    Google Scholar 

  10. Brendel, W. und W. Land: Dtsch. Med. Wschr.93, 2309 (1968).

    Google Scholar 

  11. Dausset, A. J.: Micro-Lymphocytotoxicity Techniques, Manual of Tissue typing techniques, p. 43. National Institute of Health Bethesda, Maryland/USA 1969.

    Google Scholar 

  12. Dausset, A. J., Colombani und K. Okochi: Immunological Methods. Edited by J. F.Ackroyd, Blackwell Scientific Publications, Oxford 1964.

    Google Scholar 

  13. Ehrhart, H. und E. Dörfler: Blut10, 231 (1964).

    Google Scholar 

  14. Elves M. E., Flurochromatic Cytotoxic Test. Manual of Tissue typing techniques, p. 10, National Institute of Health, Bethesda, Maryland/USA 1969.

    Google Scholar 

  15. Engelfriet, C. P., Ch. van der Weerdt and C. E. Veenhoven: Development of monospecific lymphocyte antisera. Manual of Tissue typing techniques, p. 13, National Institute of Health. Bethesda, Maryland/USA 1969.

    Google Scholar 

  16. Fallon, H. J., E. Frei, J. D. Davidson, J. S. Trier and D. Burk: J. Lab. Clin. Med.59, 779 (1962).

    Google Scholar 

  17. Fateh-Moghadam, A., R. Pichlmayr, Ch. Morell and M. Knedel: Klin. Wschr.45, 665 (1967).

    Google Scholar 

  18. Greenwood, B.: Vox Sang.15 315 (1968).

    Google Scholar 

  19. Heath, T. J., A. K. Lascelles, B. Morris: J. Anat. Ldn.96, 397 (1962).

    Google Scholar 

  20. Kissmeyer-Nielsen, F. and K. E. Kjerby: Lymphocytotoxic-Micro-Technique. Purification of lymphocytes by Flotation. Histocompatibility Testing. Report of a conference and workshop, Torino and Saint-Vincent, Italy, 14–24 June 1967.

  21. Kissmeyer-Nielsen, F. and K. E. Kjerby: Lymphnytotoxic-Micro Techniques, p. 15, National Institute of Health Bethesda, Maryland/USA 1969.

    Google Scholar 

  22. Moorhead P. S., P. C. Novell, W. J. Malman, D. M. Battipps and D. A. Hungerford: Exp. cell. Research20, 6/3 (1960).

    Google Scholar 

  23. Nagel, W., H. U. Lahann und E. Weihrauch: Klin. Wschr.36, 34 (1958).

    Google Scholar 

  24. Papermaster, B. W., V. M. Papermaster and D. Degling: Cytotoxicity Technique. Manual of Tissue typing techniques, p. 20, National Institute of Health, Bethesda, Maryland/USA 1969.

    Google Scholar 

  25. Payne, R.: Meth. Med. Research10, 27 (1964).

    Google Scholar 

  26. Payne, R.: Agglutination Technique. Manual of Tissue typing techniques, p. 34, National Institute of Health, Bethesda, Maryland/USA 1969.

    Google Scholar 

  27. Pichlmayr, R.: Z. gesamte exp. Med.143, 161 (1967).

    Google Scholar 

  28. Pichlmayr, R., W. Brendel, G. Beck, E. Schnittdiel, G. Titow und I. Pichlmayr: Klin. Wschr.45, 199 (1967).

    Google Scholar 

  29. Pichlmayr, R., W. Brendel und R. Zenker: Münch. Med. Wschr.110, 893 (1968).

    Google Scholar 

  30. Pichlmayr, R., W. Brendel, G. Beck, E. Schnittdiel, I. Pichlmayr, S. Thierfelder, A. Fateh-Moghadam und W. Land. Klin. Wschr.46, 249 (1968).

    Google Scholar 

  31. Preussner, S. und G. Frick: Das Dtsch. Gesundheitswesen24, 2050 (1969).

    Google Scholar 

  32. Rabinowitz, Y.: Blood23, 811 (1964).

    Google Scholar 

  33. Rabinowitz, Y.: Blood27, 470 (1966).

    Google Scholar 

  34. Rogentine, G. N.: 51 CR Release Cytotoxicity Technique. Manual of Tissue typing techniques, p. 21, National Institute of Health, Bethesda, Maryland USA 1969.

    Google Scholar 

  35. Rojahn, A. und A. Tolle: Monatshefte für Tierheilkunde15, 37 (1963).

    Google Scholar 

  36. Rood, van I. I.: Defined Leukocyte antigenic groups in men. Manual of Tissue typing techniques, p. 38, National Institute of Health, Bethesda, Maryland/USA 1969.

    Google Scholar 

  37. Schnappauf, H. und U. Schnappauf. Blut16, 209 (1968).

    Google Scholar 

  38. Skoog, W. A. and W. S. Beck: Blood11, 436 (1956).

    Google Scholar 

  39. Terasaki, P. I.: Microdroplet Cytotoxicity Test. Manual of Tissue typing. Technique, p. 24, National Institute of Health Bethesda, Maryland/USA 1969.

    Google Scholar 

  40. Thierfelder, S.: Vox. Sang.9, 447 (1964).

    Google Scholar 

  41. Thimm, H. J.: Inaug. Diss., Gießen 1964.

  42. Tilz, G. P., S. Sailer und R. Heschel: Med. Klinik64, 1191 (1969).

    Google Scholar 

  43. Vallee, B. L., W. L. Hughes and J. G. Gibson: Blood (special issue)1, 82 (1947)

    Google Scholar 

  44. Walford R. L.: Cytotoxicity Test. Manual of Tissue typing techniques, p. 28, National Institute of Health, Bethesda, Maryland/USA 1969.

    Google Scholar 

  45. Weinhold, E.: Berl.-Münchn.-tierärztl. Wschr.78, 406 (1965).

    Google Scholar 

  46. Woodruff, M. F. A. and U. F. Anderson: Ann. N.Y. Acad. Sci.120, 119 (1964).

    Google Scholar 

Download references

Author information

Author notes

  1. F. Otto &  D. O. Schmid

    Present address: Institut für Blutgruppen- und Resistenz forschung der Tierzuchtforschung München, München, Deutschland

Authors and Affiliations

    Authors
    1. F. Otto
      View author publications

      You can also search for this author in PubMed Google Scholar

    2. D. O. Schmid
      View author publications

      You can also search for this author in PubMed Google Scholar

    Additional information

    Die Arbeit wurde mit Unterstützung der Deutschen Forschungsgemeinschaft durchgeführt.

    Rights and permissions

    Reprints and permissions

    About this article

    Cite this article

    Otto, F., Schmid, D.O. Lymphozytenisolierung aus dem Blut des Menschen und der Tiere. Blut 21, 118–122 (1970). https://doi.org/10.1007/BF01632663

    Download citation

    • Received:

    • Issue Date:

    • DOI: https://doi.org/10.1007/BF01632663

    Search

    Navigation