Summary
A sample of 103 Italians was tested for HLA-DQA1 polymorphism using the polymerase chain reaction (PCR) and dot blot hybridization. Results were in Hardy-Weinberg equilibrium. The power of discrimination was 0.91 and the rate of exclusion 56.7. The frequencies of the DQA1*0201 and DQA1*0301 alleles were found to be significantly different from other Caucasian populations.
Zusammenfassung
Eine Stichprobe von 103 Italienern wurde auf den HLA-DQ-α Polymorphismus untersucht: die Polymerasekettenreaktion (PCR) und die Dot-Blot-Hybridisierung fanden Anwendung. Die Resultate waren im Hardy-Weinberg Gleichgewicht. Der Diskriminationsindex des Systems beträgt 0.91 und die Ausschlie-ßungschance beträgt 0.57. Die Frequenzen der Allele DQAl*0201 und DQ A1*0301 waren signifikant unterschiedlich im Vergleich zu anderen europäischen Populationen.
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References
Barros F, Vide MC, Laren MV, Carracedo A (1992) SDS-Page typing of HLA-DQA1 and pMCT118 after PCR amplification. In: Rittner Ch, Schneider P (eds) Advances in forensic haemogenetics 4. Springer, Berlin Heidelberg, pp 64–66
Bodmer JG, Marsh SGE, Parham P, Erlich HA, Alberet E, Bodner WF, Dupont B, et al. (1990) Nomenclature for factors of the HLA system, 1989. Tissue Antigens 35:1–8
Comey CT, Budowle B (1991) Validation studies on the analysis of the HLA DQa locus using the polymerase chain reaction. J Forensic Sci 36:1633–1648
Helmuth R, Fildes N, Blake E, Luce MC, Chimera J, Madej R, Gorodezky C, Stoneking M, Schmill N, Klitz W, Higuchi R, Erlich HA (1990) HLA-DQa allele and genotype frequencies in various human populations, determined by using enzymatic amplification and oligonucleotide probes. Am J Hum Genet 47:515–523
Ip N, Shaler RC, Fischer S, Van De Stadt I, Neuweiller J, King M, Bert L (1990) A method for the analysis of PCR amplification products to identify base-pair substitution and VNTR polymorphisms. In: Polesky HF, Mayr WR (eds) Advances in forensic haemogenetics 3. Springer-Verlag Berlin Heidelberg, pp 143–147
Maeda M, Murayama N, Ishii H, Uryu N, Ota M, Tsuji K, Inoko H (1989) A simple and rapid method for HLA-DQAl genotyping by digestion of PCR-amplified DNA with allele specific restriction endonucleases. Tissue Antigens 34:290–298
Ota M, Seki T, Nomura N, Sugimura K, Mizuki N, Fukushima H, Tsuji K, Inoko H (1991) Modified PCR-RFLP method for HLA-DPB1 and DQA1 genotyping. Tissue Antigens 38:60–71
Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N (1985) Enzymatic amplification of ß-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230:1350–1354
Saiki RK, Bugawan TL, Horn GT, Mullis KB, Erlich HA (1986) Analysis of enzymatically amplified beta-globin and HLADQa DNA with allele-specific oligonucleotide probes. Nature 324:163–166
Sajantila A, Strom M, Budowle B, Tienari PJ, Ehnholm C, Peltonen L (1991) The distribution of the HLA-DQα alleles and genotypes in the Finnish population as determined by the use of DNA amplification and allele specific oligonucleotides. Int J Leg Med 104:181–184
Schneider PM, Präger-Eberle M, Rittner C (1991) Zur Anwendung der Polymerase Kettenreaktion (PCR) des HLA-DQα-Systems in der forensischen Spurenkunde. Arch Kriminol 5–6:167–174
Tamaki K, Yamamoto T, Uchihi R, Katsumata Y, Kondo K, Mizumo S, Kimura A, Sasazuki T (1991) Frequency of HLADQA1 alleles in the Japanese population. Hum Hered 41:209–214
Uryu N, Maeda M, Ota M, Tsuji K, Inoko H (1990) A simple and rapid method for HLA DRB and DQB typing by digestion of PCR-amplified DNA with allele specific restriction endonucleases. Tissue Antigens 35:20–31
Walsh PS, Metzger DA, Iguchi R (1991) Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10:506–513
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Tagliabracci, A., Giorgetti, R., Agostini, A. et al. Frequency of HLA DQA1 alleles in an Italian population. Int J Leg Med 105, 161–164 (1992). https://doi.org/10.1007/BF01625169
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DOI: https://doi.org/10.1007/BF01625169