Abstract
Immunoreactivity of proliferating cell nuclear antigen (PCNA) was assessed to evaluate growth potential in surgically resected tissue specimens from 70 cases of benign and malignant salivary gland tumours. Three stage streptavidin-biotin immunoperoxidase immunostaining using monoclonal antibody to PCNA showed a heterogeneity of PCNA index and distribution. In normal salivary gland specimens, PCNA was demonstrated in the nuclei of few ductal and acinar cells. In pleomorphic adenoma a multiple nodular growth pattern was observed with positive immunoreactivity restricted to the nuclei of tubulo-ductal structures. Warthin's tumour had positive nuclei in the outer cuboidal cells of epithelial component and germinal centres of lymphoid tissue. Myoepithelioma and acinic cell carcinoma showed slightly differing values and a statistically significant difference in the value of the index was observed in tumour cell aggregates of the cribiform type of adenoid cystic carcinoma and the solid undifferentiated type and between low/intermediate and high-grade mucoepidermoid tumours. PCNA is a useful marker of tumour cell proliferation; the index correlates with the grade of malignancy in salivary gland tumours.
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Aaltomaa S, Lipponen Pm, Syrjanen K (1992) Prognostic value of cell proliferation in breast cancer as detected by proliferating cell nuclear antigen (PCNA) immunostaining. Anticancer Res 12:1281–1286
Battersby S, Anderson JJ (1990) Correlation of proliferative activity in breast tissue using PCNA/cyclin. Hum Pathol 21:781
Bravo R, Frank R, Blundell PA, MacDonald-Bravo H (1987) Cyclin/PCNA is the auxiliary protein of DNA polymerase delta. Nature 309:515–520
Dierendock JHV, Wijsman RK, Keizer R, Velde CJH van de, Cornelisse CJ (1991) Cell cycle related staining pattern of antiproliferating cell nuclear antigen monoclonal antibodies: comparison with BrdU labeling and Ki-67 staining. Am J Pathol 138:1165–1172
Figge C, Reinfenberger G, Vogeley KT, Messing M, Roosen N, Wechsler W (1992) Immunohistochemical demonstration of proliferating cell nuclear antigen in glioblastomas: pronounced heterogeneity and lack of prognostic significance. J Cancers Res Clin Oncol 118:289–295
Garcia RL, Coltrera MD, Gown AM (1989) Analysis of proliferative grade using PCNA/cyclin monoclonal antibodies in fixed, embedded tissues — comparison with flow cytometric analysis. Am J Pathol 134:733–739
Hall PA, Levison DA, Woods AL, Yu CC-W, Kellock DB, Watkins JA, Barnes DM, Gillett CE, Camplejohns R, Dover R, Waseem NH, Lane DP (1990) Proliferating cell nuclear antigen (PCNA immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms. J Pathol 162:285–294
Harper ME, Glynne-Jones E, Goddard L, Wilson DW, Metenhelia SS, Conn IG, Peeling WB, Griffiths K (1992) Relationship of proliferating cell nuclear antigen (PCNA) in prostatic carcinoma to various clinical parameters. Prostate 20:243–253
Ito M, Tsurusawa M, Zha Z, Kawai S, Takasaki I, Fujimoto T (1992) Cell proliferation in childhood acute leukemia. Comparison of Ki-67 and proliferating cell nuclear antigen immunocytochemical and DNA flow cytometric analysis. Cancer 69:2176–2182
Jain S, Filipe MI, Hall PA, Waseem N, Lane DP, Levison DA (1991) Prognostic value of proliferating cell nuclear antigen in gastric carcinoma. J Clin Pathol 44:655–659
Jaskulski D, deRiel JK, Mercer WE, Calbretta B, Baserga R (1988) Inhibition of cellular proliferation by antisense oligodeoxynucleotides to PCNA/cyclin. Science 240:1544–1546
Kurki P, Vanderlaan M, Dolbeare F, Grey J, Tan EM (1986) Expression of proliferating cell nuclear antigen (PCNA/cyclin) during the cell cycle. Exp Cell Res 166:209–219
Leonardi E, Girlando S, Serio G, Mauri FA, Perrone G, Scampini S, Dall Palma P, Barbareschi M (1992) PCNA and Ki67 expression in breast carcinoma: correlations with clinical and biological variables. J Clin Pathol 45:416–419
Lipponen PK, Eskelinen MJ (1992) Cell proliferation of transitional cell bladder tumours determined by PCNA/cyclin immunostaining and its prognostic value. Br J Cancer 66:171–176
Morris GF, Mathews MB (1989) Regulation of proliferating cell antigen during cell cycle. J Biol Chem 264:13856–13864
Seifert G, Brocheriou C, Cardesa A, Eveson JW (1990) WHO international histological classification of tumors. Tentative histological classification of salivary gland tumours. Pathol Res Pract 186:555–581
Shrestha P, Yamada K, Wada T, Maeda S, Watatani M, Yasutomi M, Takagi H, Mori M (1992) Proliferating cell nuclear antigen in breast lesions: correlation of c-erbB-2 oncoprotein and EGF receptor and its clinicopathologic significance in breast cancer. Virchows Arch [A] 421:193–202
Theunissen PH, Leers MP, Bollen EC (1992) Proliferating cell nuclear antigen (PCNA) expression in formalin-fixed tissue of non-small cell lung carcinoma. Histopathology 20:251–255
Tsuji T, Shrestha P, Yamada K, Takagi H, Shinozaki F, Sasaki K, Maeda K, Mori M (1992) Proliferating cell nuclear antigen in malignant and pre-malignant lesions of epithelial origin in the oral cavity and skin: an immunohistochemical study. Virchows Arch [A] 420:377–383
Visakorpi T, Kallioniemi OP, Heikkinen A, Koivula T, Isola J (1992) Small subgroup of aggressive, highly proliferative prostatic carcinomas defined by p53 accumulation. J Natl Cancer Inst 84:883–887
Wong RL, Katz ME, Ogata K, Tan EM, Cohen S (1987) Inhibition of nuclear DNA synthesis by an auto-antibody to proliferating cell nuclear antigen/cyclin. Cell Immunol 110:443–448
Woods AL, Hall PA, Shepherd NA, Hanby AM, Waseem NH, Lane DP, Levison DA (1991) The assessment of proliferating cell nuclear antigen (PCNA) immunostaining in primary gastrointestinal lymphoma and its relationship to histological grade, S+G2+M phase fraction (flow cytometric analysis) and prognosis. Histopathology 19:21–27
Yu CC, Hall PA, Fletcher CD, Camplejohn RS, Waseem NH, Lane DP, Levison PA (1991) Hemangiopericytomas: the prognostic value of immunohistochemical staining with a monoclonal antibody to proliferating cell nuclear antigen (PCNA). Histopathology 19:29–33
Yu CC, Fletcher CD, Newman PA, Goodland JR, Burton JC, Levison DA (1992) A comparison of proliferating cell nuclear antigen (PCNA) immunostaining, nucleolar organizer region (AgNOR) staining, and histologic grading in gastrointestinal stromal tumors. J Pathol 166:147–152
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Yang, L., Hashimura, K., Qin, C. et al. Immunoreactivity of proliferating cell nuclear antigen in salivary gland tumours: An assessment of growth potential. Vichows Archiv A Pathol Anat 422, 481–486 (1993). https://doi.org/10.1007/BF01606457
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DOI: https://doi.org/10.1007/BF01606457