Summary
Renal arteriolar diameters were measured, using microvascular resin casts, in two hyperfiltration models of rats: the remnants kidney of subtotal nephrectomy (NX) and streptozotocin-induced diabetic kidney (DM). In the NX, the blood pressure was elevated, urinary protein excretion was markedly increased and glomeruli were severely damaged. In the DM, although the blood pressure remained normal, urinary protein excretion was significantly increased and glomeruli were damaged but to a lesser extent than in the NX group. In the NX group, the afferent arteriole was dilated and the efferent arteriole was constricted. In the DM group, the afferent arteriole was dilated, while the efferent arteriole remained unchanged. The results showed that afferent arteriolar dilatation was seen in both the NX and DM groups, possibly leading to the glomerular damage. In the NX group, the systemic high blood pressure and efferent arteriolar constriction augmented glomerular damage significantly.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bank N, Lahorra MA, Aynedjian HS (1987) Acute effect of calcium and insulin on hyperfiltration of early diabetes. Am J Physiol 252:E12-E20
Bank N, Lahorra MA, Aynedjian HS, Schlondorff D (1988) Vasoregulatory hormones and the hyperfiltration of diabetes. Am J Physiol 254:F202-F209
Brenner BM (1985) Nephron adaptation to renal injury to ablation. Am J Physiol 249:F324-F337
Brenner BM, Dworkin LD, Ichikawa I (1986) Glomerular filtration. In: Brenner BM, Rector FC (eds) The kidney, vol I. Saunders, Philadelphia, pp 124–144
Cooper ME, Allen TJ, O'Brien RC, Macmillan PA, Clarke B, Jerums G, Doyle AE (1988) Effects of genetic hypertension on diabetic nephropathy in the rat —functional and structural characteristics. J Hypertens 6:1009–1016
Dilley JR, Stier CT, Arendshorst WJ (1984) Abnormalities in glomerular function in rats developing spontaneous hypertension. Am J Physiol 246:F12-F20
Gattone VH, Evans AP, Willis LR, Luft FC (1983) Renal afferent arteriole in the spontaneously hypertensive rat. Hypertension 5:8–16
Herrera-Acosta J, Tapia E, Bobadilla NA, Romero L, Cermeno JL, Alvarado JA, Gabbai FB (1988) Evaluating hyperfiltration with glycine in hypertensive rats with renal ablation. Hypertension 11:I-33–I-37
Hostetter TH, Olsen JL, Rennke HG, Venkatachalam MA, Brenner BM (1981) Hyperfiltration in remnant nephrons: a potentially adverse response to renal ablation. Am J Physiol 241:F85-F93
Jackson B, Johnston CI (1988) The contribution of systemic hypertension to progression of chronic renal failure in the rat remnant kidney: effect of treatment with an angiotensin converting enzyme inhibitor or a calcium inhibitor. J Hypertens 6:495–501
Jensen PK, Christiansen JS, Steven K, Parving HH (1981) Renal function in streptozotocin-diabetic rats. Diabetologia 21:409–414
Jensen PK, Christiansen JS, Steven K, Parving HH (1987) Strict metabolic control and renal function in the streptozotocin diabetic rat. Kidney Int 31:47–51
Kimura K, Nanba S, Tojo A, Hirata Y, Matsuoka H, Sugimoto T (1989) Variations in arterioles in spontaneously hypertensive rats. Morphometric analysis of afferent and efferent arterioles. Virchows Arch [A] 415:565–569
Kimura K, Hirata Y, Matsuoka H, Ishii M, Sugimoto T, Kangawa K, Matsuo H. Drozdoava G (1990) Effects of chronic administration of atrial natriuretic polypeptide on glomerular lesions in spontaneously hypertensive rats. Jpn Heart J 31:227–236
Martinez-Maldonado M, Rodriguez-Sargent C, Cangiano JL, Dworkin LD (1987) Pathogenesis of systemic hypertension and glomerular injury in the spontaneously hypertensive rat. Am J Cardiol 60:471–521
Ohkubo A, Kamei S, Yamanaka M, Arai F, Kitajima M, Kondo A (1981) Plasma glucose concentrations of whole blood, as determined with a multilayer-film analytical element. Clin Chem 27:1287–1290
Ortola FV, Ballermann BJ, Anderson S, Mendez RE, Brenner BM (1987) Elevated plasma atrial natriuretic peptide levels in diabetic rats. J Clin Invest 80:670–674
Volkmann HP, Wehner H (1986) Renal vessel changes in diabetic KK-mice. Virchows Arch [A] 409:669–678
Watanabe N, Kamei S, Ohkubo A, Yamanaka M, Ohsawa S, Makino K, Tokuda K (1986) Urinary protein as measured with a pyrogallol red-molybdate complex, manually and in a Hitachi 726 automated analyzer. Clin Chem 32:1551–1554
Wiseman MJ, Mangili R, Alberetto M, Keen H, Viberti G (1987) Glomerular response mechanisms to glycemic changes in insulin-dependent diabetics. Kidney Int 31:1012–1018
Yoshioka T, Shiraga H, Yoshida Y, Fogo A, Glick AD, Deen WM, Hoyer JR, Ichikawa I (1988) “Intact nephrons” as the primary origin of proteinuria in chronic renal disease. Study in the rat model of subtotal nephrectomy. J Clin Invest 82:1614–1623
Zatz R, Meyer TW, Rennke HG, Brenner BM (1985) Predominance of hemodynamic rather than metabolic factors in the pathogenesis of diabetic glomerulopathy. Proc Natl Acad Sci USA 82:5963–5967
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kimura, K., Tojo, A., Nanba, S. et al. Morphometric analysis of arteriolar diameters in experimental nephropathies: Application of microvascular casts. Vichows Archiv A Pathol Anat 417, 319–323 (1990). https://doi.org/10.1007/BF01605783
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01605783