Summary
The immunohistological distribution of collagen types I, II, III, V and VI in human benign and malignant cartilaginous tumours of bone was examined with regard to their aggressiveness. The matrix of enchondromas consisted of type II collagen distributed diffusely, and type VI predominantly localized in the immediate surroundings of the cells. Types I, III and V collagen were not found. These findings were similar to the distribution of collagenous proteins in normal hyaline cartilage where each lobule was consistently rimmed by types I and V collagen. In grade 1 chondrosarcomas, the main collagenous components of matrix were also types II and VI collagen. Type II was sometimes found in the cytoplasm of tumour cells and type VI tended to lose territorial localization. In addition, type I collagen was demonstrated consistently and type V in some cases. In grade 2 chondrosarcomas, type II collagen was demonstrated not only in the matrix but occasionally in the cytoplasm of tumour cells. Type VI was dispersed in the intercellular areas. The other collagenous proteins such as types I, III and V were also present in the matrix. In grade 3 chondrosarcomas, type II collagen was localized predominantly in the cytoplasm of tumour cells and in the adjacent matrix. Type VI was markedly decreased with complete loss of pericellular distribution, whereas types I, III and V were constantly present in the matrix. Those alterations in the distribution of collagen types correlated well with the aggressive behaviour of the tumours. The findings suggest that distribution of different collagen types in cartilaginous tumours reflects the immaturity of the tumour cells and is a useful indicator of their aggressiveness.
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References
Ayad S, Evans H, Weiss JB, Holt L (1984) Type VI collagen but not type V collagen is present in cartilage. Coll Rel at Res 4:165–168
Burgeson RE (1988) New collagens, new concepts. Annu Rev Cell Biol 4:551–577
Enneking WF (1986) A system of staging musculoskeletal neoplasms. Clin Orthop Relat Res 204:9–24
Eriksson AI, Schiller A, Mankin HJ (1980) The management of chondrosarcoma of bone. Clin Orthop Relat Res 153:44–66
Evans HL, Ayala AG, Romsdahl MM (1977) Prognostic factors in chondrosarcoma of bone. A clinicopathologic analysis with emphasis on histologic grading. Cancer 40:818–831
Gordon MK, Gerecke DR, Olsen BR (1987) Type XII collagen: Distinct extracellular matrix component discovered by cDNA cloning. Proc Natl Acad Sci USA 84:6040–6044
Herwig J, Roessner A, Buddecke E (1986) Isolation and characterization of proteoglycans and glycosoaminoglycans from human chondrosarcoma. Exp Mol Pathol 45:118–127
Hsu SM, Raine L, Fanger H (1981) A comparative study of the peroxidase-antiperoxidase method and avidin-biotin complex method for studying polypeptide hormones with radioimmunoassay antibody. Am J Clin Pathol 75:734–738
Kreicbergs A, Boquist L, Borssen B, Larsson SE (1982) Prognostic factors in chondrosarcoma. A comparative study of cellular DNA content and clinicopathologic features. Cancer 50:577–583
Kwan APL, Dickson IR, Freemont AJ, Grant ME (1989) Comparative studies of type X collagen expression in normal and rachitic chicken epiphyseal cartilage. J Cell Biol 109:1849–1856
Mankin HJ, Cantley KP, Lippiello L, Schiller AL, Campbell CJ (1980a) The biology of human chondrosarcoma. I. Description of the cases, grading, and biochemical analysis. J Bone Joint Surg[Am] 62:160–176
Mankin HJ, Cantley KP, Schiller AL, Lippielo L (1980b) The biology of human chondrosarcoma. II. Variation in chemical composition among types and subtypes of benign and malignant cartilage tumors. J Bone Joint Surg [Am] 62:176–188
Mark K von der, Mark H von der (1977) The role of three genetically distinct collagen types in enchondral ossification and calcification of cartilage. J Bone Joint Surg [Br] 59:458–464
Matsuno T (1979) Morphological and biochemical studies of collagen on benign and malignant cartilage tumors (in Japanese). Hokkaido Assoc Orthop Traum 24:1–15
Mendeer M, Eich-Bender SG, Vaughan L, Winterhalter KH, Bruckner P (1989) Cartilage contains mixed fibrils of collagen type II, IX, and XL J Cell Biol 108:191–197
Miller EJ (1985) Recent Information on the chemistry of the collagens. In: Proceedings of 2nd international Conference on the Biochemistry and Biology of Mineralized Tissues. Ebsco Media, Birmingham, Ala., pp 80–93
Miller EJ, Gay S (1987) The collagens: an overview and update. Methods Enzymol 144:3–41
Mirra JM, Gold R, Downs J, Eckardt JJ (1985) A new histologic approach to the differentiation of enchondroma and chondrosarcoma of the bones. A clinicopathologic analysis of 51 cases. Clin Orthop Relat Res 201:214–237
Oda Y, Kawahara E, Minamoto T, Ueda Y, Ikeda K, Nagai Y, Nakanishi I (1988) Immunohistochemical studies on the tissue localization of collagen types I, III, IV, V, and VI in schwannomas. Correlation with ultrastructural features of the extracellular matrix. Virchows Arch [B] 56:153–163
Poole CA, Wotton SF, Daunce VC (1988) Localization of type IX collagen in chondrons isolated from porcine articular cartilage and rat chondrosarcoma. Histochem J 20:567–574
Reddi AH, Gay R, Gay S, Miller EJ (1977) Transitions in collagen types during matrix-induced cartilage, bone, and bone marrow formation. Proc Natl Acad Sci USA 74:5589–5592
Remberger K, Gay S (1977) Immunohistochemical demonstration of different collagen types in the normal epiphyseal plate and in benign and malignant tumors of bone and cartilage. Z Krebsforsch 90:95–106
Sandberg M, Tamminen M, Hirvonen H, Vuorio E, Philajaniemi T (1989) Expression of mRNAs coding for the α1 chain of type XIII collagen in human fetal tissues: comparison with expression of mRNAs for collagen types I, II, and III. J Cell Biol 109:1371–1379
Schmid TM, Linsenmayer TF (1985) Developmental acquisition of type X collagen in the embryonic chick tibiotarsus. Dev Biol 107:373–381
Sweet MBE, Thonar EJ-MA, Immelman AR (1976) Glycoso-aminoglycans and proteoglycans of human chondrosarcoma. Biochem Biophys Acta 437:71–86
Tacchetti C, Quarto R, Nitsch L, Hartmann DJ, Cancedda R (1987) In vitro morphogenesis of chick embryo hypertrophic cartilage. J Cell Biol 105:999–1006
Ueda Y, Nakanishi I (1989) Immunohistochemical and biochemical studies on the collagenous proteins of human osteosarcomas. Virchows Arch [B] 58:79–88
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Ueda, Y., Oda, Y., Tsuchiya, H. et al. Immunohistological study on collagenous proteins of benign and malignant human cartilaginous tumours of bone. Vichows Archiv A Pathol Anat 417, 291–297 (1990). https://doi.org/10.1007/BF01605779
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DOI: https://doi.org/10.1007/BF01605779