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Immunocytochemical evidence for SV40-related T antigen in two human brain tumours of ependymal origin

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Summary

A series of thirty-nine human brain tumours has been screened for the presence or absence of SV40-related T antigen by the direct and indirect immunoperoxidase techniques. Two tumours (an ependymoma and a choroid plexus papilloma) of ependymal origin revealed markedly positive nuclear staining for T antigen both in in vivo and in vitro. The possible viral etiology of these human brain tumours is discussed in relation to recent human papovavirus isolates.

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References

  • Becker, L. E., Narayan, O., Johnson, R. T., Studies of human papovavirus tumor antigen in experimental and human cerebral neoplasms. Can. J. Neurol. Sci.3 (1976), 105–109.

    Google Scholar 

  • Black, P. H., Rowe, W. P., Turner, H. C., Huebner, R. J., A specific complement-fixing antigen present in SV40 tumor and transformed cells. Proc. Natl. Acad. Sci.50 (1963), 1148–1156.

    Google Scholar 

  • Brugge, J. S., Butel, L., Role of Simian virus 40 A gene function in maintenance of transformation. J. Virol.15 (1975), 619–635.

    Google Scholar 

  • Busch, H., Isolation and purification of nuclei. In: Colowick, S. P., and Kaplan, N. O. (ed.), Methods in Enzymology, Vol. 12. Part A, pp. 421–448. New York: Academic Press. 1967.

    Google Scholar 

  • Castaigne, P., Rondot, P., Escourolle, R., Ribadeau Dumas, J. L., Cathala, F., Hauw, J. J., Leucoencephalopathie multifocale progressive et “gliomes” multiples. Rev. Neurol.130 (1974), 379–392.

    Google Scholar 

  • Costa, J., Yee, C., Tralka, T. S., Rabson, A. S., Hamster ependymomas produced by intracerebral inoculation of a human papovavirus (MMV). J. Natl. Cancer Inst.56 (1976), 863–864.

    Google Scholar 

  • Dougherty, R. M., DiStefano, H. S., Isolation and characterization of a papova-virus from human urine. Proc. Soc. Exp. Biol. Med.146 (1974), 481–487.

    Google Scholar 

  • —, A comparison of human papovavirus T antigens. J. gen. Virol.33 (1976), 61–70.

    Google Scholar 

  • —, Induction of tumors in Syrian hamsters by a human renal papovavirus, RF strain. J. Natl. Cancer Inst.57 (1976), 395–400.

    Google Scholar 

  • Gardner, S. D., Field, A. M., Coleman, D. V., Hulme, B., New human papovavirus (B.K.) isolated from urine after renal transplantation. Lancet1 (1971), 1253–1257.

    Google Scholar 

  • Graham, R. C., Jr., Karnovsky, M. J., The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: Ultrastructural cytochemistry by a new technique. J. Histochem. Cytochem.14 (1966), 291–302.

    Google Scholar 

  • Greenlee, J. E., Narayan, O., Johnson, R. T., Herndon, R. M., Induction of brain tumors in hamsters with BK virus, a human papovavirus. Lab. Investigation36 (1977), 636–641.

    Google Scholar 

  • Howley, P. M., Khoury, G., Takemoto, K. K., Martin, M. A., Polynucleotide sequence common to the genomes of simian virus 40 and the human papova- viruses JC and BK. Virology73 (1976), 303–307.

    Google Scholar 

  • Ikuta, F., Kumanishi, T., Experimental virus-induced brain tumors. In: Zimmerman, H. M. (ed.), Progress in Neuropathology, pp. 253–334. New York and London: Grune & Stratton. 1973.

    Google Scholar 

  • Lecatsas, G., Prozesky, O. W., Wan Wyk, J., Els, H. J., Papovavirus in urine after renal transplantation. Nature241 (1973), 343–344.

    Google Scholar 

  • Lewis, A. M., Jr., Levine, M. J., Wiese, W. H., Crumpacker, C. S., Henry, P. H., A nondefective (competent) adenovirus-SV40 hybrid isolated from the AD2-SV40 hybrid population. Proc. Natl. Acad. Sci.63 (1969), 1128–1135.

    Google Scholar 

  • —, Rowe, W. P., Studies on non-defective Ad-SV40 hybrid viruses: I. A newly characterized SV40 antigen induced by the Ad2+ND, genome. J. Virol.7 (1971), 189–197.

    Google Scholar 

  • Lin, P. S., Schmidt-Ulrich, R., Wallach, F. H., Transformation by simian virus 40 induces virus-specific, related antigens in the surface membrane and nuclear envelope. Proc. Natl. Acad. Sci.74 (1977), 2495–2499.

    Google Scholar 

  • Näse, L. H., Kärkkäinen, M., Mäntyjärvi, R. A., Transplantable hamster tumors induced with the BK virus. Acta path. microbiol. scand., Sect. B83 (1975), 347–352.

    Google Scholar 

  • Osborn, M., Weber, K., Simian virus 40 gene A function and maintenance of transformation. J. Virol.15 (1975), 635–644.

    Google Scholar 

  • Padgett, B. L., Walker, D., ZuRhein, G. M., Eckroade, R. J., Dessel, B. H., Cultivation of papova-like virus from human brain with progressive multifocal leucoencephalopathy. Lancet1 (1971), 1257–1260.

    Google Scholar 

  • Rabotti, F., Experimental intracranial tumors of viral etiology. In: Kirsch, W. M., Paoletti, E. G., Paoletti, P. (ed.), The experimental Biology of Brain Tumors, pp. 148–180. Springfield, Ill.: Ch. C Thomas. 1972.

    Google Scholar 

  • Rapp, F., Kitahara, T., Butel, J. S., Melnick, J. L., Synthesis of SV 40 tumor antigen during replication of simian papovavirus (SV 40). Proc. Natl. Acad. Sci.52 (1974), 1138–1142.

    Google Scholar 

  • Shah, K. V., Evidence for an SV 40-related papovavirus infection of man. Amer. J. Epidemiol.95 (1972), 199–206.

    Google Scholar 

  • —, Daniel, R. W., Strandberg, J. D., Sarcoma in a hamster inoculated with BK virus, a human papovavirus. J. Natl. Cancer Inst.54 (1975), 945–950.

    Google Scholar 

  • Shein, H. M., Transformation of astrocytes and destruction of spongioblasts induced by a simian tumor virus SV40 in cultures of human fetal neuroglia. J. Neuropathol. Exp. Neurol.26 (1967), 60–76.

    Google Scholar 

  • Shiraki, H., Slow virus infection of human nervous system. In: Hama, K., Ootani, S., Nagai, K. (ed.), Brain and Nerve, pp. 211–236. Tokyo: Bunkodo. 1973. (In Japanese).

    Google Scholar 

  • Soriano, F., Shelburne, C. E., Gükcen, M., Simian virus 40 in a human cancer. Nature249 (1976), 421–424.

    Google Scholar 

  • Tabuchi, K., Lehman, J. M., Kirsch, W. M., Immunocytochemical localization of simian virus 40 T-antigen with peroxidase-labeled antibody fragments. J. Virol.17 (1976), 668–671.

    Google Scholar 

  • Takemoto, K. K., Rabson, A. S., Mullarkey, M. F., Blaese, R. M., Garon, C. F., Nelson, D., Isolation of papovavirus from brain tumor and urine of a patient with Wiskott-Aldrich Syndrome. J. Natl. Cancer Inst.53 (1974), 1205–1207.

    Google Scholar 

  • Tegtmeyer, P., Function of simian virus 40 gene A in transforming infection. J. Virol.15 (1975), 613–618.

    Google Scholar 

  • Tevethia, S. S., Rapp, F., Comparative immunology of carcinogenesis by DNA viruses. In: Hanna, M. G., Jr., and Rapp, F. (ed.), Contemporary Topics in Immunology, Vol. 6, pp. 1–69. New York: Plenum Press. 1977.

    Google Scholar 

  • Tooze, J., The Molecular Biology of Tumor Viruses, Cold Spring Harbor Laboratory, pp. 305–402. New York. 1973.

    Google Scholar 

  • Walker, D. L., Padgett, B. L., ZuRhein, G. M., Human papovavirus (JC): Induction of brain tumors in hamsters. Science181 (1973), 674–676.

    Google Scholar 

  • — — —, Albert, A. D., Marsh, R. F., Current study of an opportunistic papova-virus. In: W. Zeman, Lennette, E. H., Bruson, J. G. (eds.), Slow Viruses, pp. 49–58. Baltimore, Md.: Williams and Wilkins. 1973.

    Google Scholar 

  • Wasserman, E., Levine, L., Quantitative micro-complement fixation and its use in the study of antigenic structure by specific antigen-antibody inhibition. J. Immunol.78 (1961), 290–295.

    Google Scholar 

  • Weiner, L. P., Herndon, R. M., Narayan, O., Johnson, R. T., Shah, K., Rubinstein, L. J., Preziosi, T. J., Conley, F. K., Isolation of virus related to SV 40 from patients with progressive multifocal leucoencephalopathy. New Engl. J. Med.286 (1972), 385–390.

    Google Scholar 

  • —, Narayan, O., Penney, J. B., Herndon, R. M., Feringa, E. R., Tourtellotte, W. W., Johnson, R. T., Papovavirus of JC type in progressive multifocal leucoencephalopathy. Arch. Neurol.29 (1973), 1–3.

    Google Scholar 

  • — —, Virologic studies of progressive multifocal leucoencephalopathy. Prog. Med. Virol.18 (1974), 229–240.

    Google Scholar 

  • Weiss, A. F., Portmann, R., Fischer, H., Simon, J., Zang, K. D., Simian virus 40-related antigens in three human meningiomas with defined chromosome loss. Proc. Natl. Acad. Sci.72 (1975), 609–613.

    Google Scholar 

  • —, Birkmayer, G. D., Miller, F., SV40 related papova-viruses in human meningiomas. Acta Neuropath.34 (1976), 171–174.

    Google Scholar 

  • Varakis, J. N., ZuRhein, G. M., Experimental pineocytoma of the Syrian hamster induced by a human papovavirus (JC). Acta Neuropath.35 (1976), 243–264.

    Google Scholar 

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Author notes

  1. K. Tabuchi

    Present address: Department of Neurological Surgery, Okayama University Medical School, Okayama, Japan

Authors and Affiliations

  1. Department of Neurological Surgery, Okayama University Medical School, Okayama, Japan

    W. M. Kirsch & J. J. Van Buskirk

  2. Department of Neurosurgery, University of Colorado Medical Center, Denver, Colorado, USA

    K. Tabuchi, W. M. Kirsch & J. J. Van Buskirk

Authors
  1. K. Tabuchi
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  2. W. M. Kirsch
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  3. J. J. Van Buskirk
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Additional information

This work was supported by Pediatric Oncology Grant, POI Ca 12247-05, a grant from the Veteran's Administration, #4984-01, CC103, FCP 102(1371) and a National Institutes of Health Grant AI-01909.

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Tabuchi, K., Kirsch, W.M. & Van Buskirk, J.J. Immunocytochemical evidence for SV40-related T antigen in two human brain tumours of ependymal origin. Acta neurochir 43, 239–249 (1978). https://doi.org/10.1007/BF01587959

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