Abstract
Halophilic microorganisms were isolated from Triassic and Permian salt deposits. Two were rods and grew as red colonies; another was a coccus and produced pink colonies. The rods lysed in solutions that lacked added sodium chloride. Growth of all isolates was inhibited by aphidicolin and their bulk proteins were acidic as judged from isoelectric focusing. Therefore, these organisms were tentatively identified as extreme halophiles. Whole cell proteins patterns of the isolates following gel electrophoresis were distinct and differed from those of representative type strains of halophilic bacteria. The membrane ATPases from the rods were similar to the enzyme fromHalobacterium saccharovorum with respect to subunit composition, enzymatic properties and immunological cross-reaction, but differed slightly in amino acid composition. If the age of the microbial isolated is similar to that of the salt deposits, they can be considered repositories of molecular information of great evolutionary interest.
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Arthurton, R. S.: 1973,Sedimentology 20, 145–160.
Bien, E. and Schwartz, W.: 1965,Z. Allg. Mikrobiol. 5, 185–205.
Bradford, M. M.: 1976,Anal. Biochem. 72, 248–254.
Dombrowski, H. J.: 1963,Ann. New York Acad Sci. 108, 477–484.
Eimhjellen, K.: 1965,Zbl. Bakt., Parasitenk., Infekt., Hyg., Abt. 1, Suppl.1, 126–137.
Fillingame, R. H.: 1990, inThe Bacteria, Vol XII, Bacterial Energetics, T. A. Krulwich, (ed.), Academic Press, San Diego.
Gogarten, J. P., Kibak, H., Dittrich, P., Taiz, L., Bowman, E. J., Bowman, B. J., Manolson, M. F., Poole, R. J., Date, T., Oshima, T., Konishi, J., Denda, K., and Yoshida, M.: 1989,Proc. Natl. Acad. Sci. U.S.A. 86, 6661–6665.
Harlow, E. and Lane, D.: 1988,Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory, New York.
Hilpert, R., Winter, J., Hammes, W., and Kandler, O.: 1981,Zbl. Bakt. Hyg., I. Abt. Orig. C 2, 11–20.
Hochstein, L. I., Kristjansson, H., and Altekar, W.: 1987,Biochem. Biophys. Res. Commun. 147, 295–300.
Ihara, K. and Mukohata, Y.: 1991,Arch. Biochem. Biophys. 286, 111–116.
Klaus, W.: 1974,Carinthia II, 164/Jahrg.84, 79–85, Klagenfurt.
Klaus, W.: 1987,Einführung in die Pal äobotanik, Band I. F. Deuticke Verlag, Vienna.
Kristjansson, H. and Hochstein, L. I.: 1985,Arch. Biochem. Biophys. 241, 590–595.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J.: 1951,J. Biol. Chem. 193, 265–275.
Nanba, T. and Mukohata, Y.: 1987,J. Biochem.102, 591–598.
Norton, C. F. and Grant, W. D.: 1988,J. Gen. Microbiol. 134, 1365–1373.
Norton, C. F.: 1988, Abstr. I-84, Annu. Meetg. Am. Soc. Microbiol., Am. Soc. Microbiol., Washington.
Norton, C. F.: 1989, Abstr. I-108, Annu. Meetg. Am. Soc. Microbiol., Am. Soc. Microbiol., Washington.
Pak, E. and Schauberger, O.: 1981,Verh. Geol. B.-A., Jahrg. 1981, 185–192.
Reiser, R. and Tasch, P.:: 1960,Trans. Kansas Acad. Sci. 60, 31–34.
Reistad, R.: 1970,Arch. Mikrobiol. 71, 353–360.
Rippel, A.: 1935,Arch. Mikrobiol. 6, 350–358.
Ross, H. N. M., Collins, M. D., Tindall, B. J., and Grant, W. D.: 1981,J. Gen. Microbiol. 123, 75–80.
Ross, H. N. M., Grant, W. D., and Harris, J. E.: 1985, inChemical Methods in Bacterial Systematics, Goodfellow, M. and Minnekin, D. E., (eds.), Acad. Press, London, New York.
Schinzel, R. and Burger, K. J.: 1984,FEMS Microbiol. Lett. 25, 187–190.
Sonnenfeld, P.: 1984,Brines and Evaporites, Academic Press, Orlando, Florida.
Staley, J. T., Bryant, M. P., Pfennig, N., and Holt, J. G.: 1989,Bergey's Manual of Systematic Bacteriology, Vol. 3. Willams and Wilkins, Baltimore.
Stan-Lotter, H., and Hochstein, L. I.: 1989,Eur. J. Biochem. 179, 155–160.
Stan-Lotter, H., Lang, F. J. Jr., and Hochstein, L. I.: 1989,Appl. Theoret. Electroph. 1, 147–153.
Stan-Lotter, H., Bowman, E. J., and Hochstein, L. I.: 1991,Arch. Biochem. Biophys. 284, 116–119.
Tomlinson, G. A. and Hochstein, L. I.: 1972,Can. J. Microbiol. 18, 698–701.
Tomlinson, G. A. and Hochstein, L. I.: 1976,Can. J. Microbiol. 22, 587–591.
Woese, C. R.: 1987,Microbiol. Rev. 51, 221–271.
Woese, C. R. and Fox, G. E.: 1977,Proc. Natl. Acad. Sci. U.S.A. 74, 5088–5090.
Woese, C. R., Kandler, O., and Wheelis, M. L.: 1990,Proc. Nat. Acad. Sci. U.S.A. 87, 4576–4579.
Zharkov, M. A.: 1981,History of Paleozoic Salt Accumulation, Springer Verlag, Berlin, Heidelberg, New York.
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Stan-Lotter, H., Sulzner, M., Egelseer, E. et al. Comparison of membrane ATPases from extreme halophiles isolated from ancient salt deposits. Origins Life Evol Biosphere 23, 53–64 (1993). https://doi.org/10.1007/BF01581990
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DOI: https://doi.org/10.1007/BF01581990