Abstract
The glycoproteins and glycolipids from membranes of virulent strain Z and avirulent strain M ofMycoplasma hyopneumoniae have been compared. The proteins and the glycoproteins were identified by SDS-polyacrylamide gel electrophoresis and concanavalin A-biotin labeling, respectively. The membrane preparation contained approximately 34 protein bands with molecular weights between 20 KD and 100 KD. The concanavalin A-biotin system reacted with a glycoprotein of a molecular weight of approximately 28,000 from avirulent strain M and did not react with the correspondent band from virulent strain Z. The membrane glycolipids of both strains consisted of monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG), and the percentages of 16∶0, 18∶0, and 18∶1 fatty acids comprised more than 80% of the total fatty acids of membrane glycolipids. The 18∶0 fatty acid of MGDG in avirulent strain M was twofold higher than that of virulent strain Z.
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Chen, J.W., Zhang, L., Song, J. et al. Comparative analysis of glycoprotein and glycolipid composition of virulent and avirulent strain membranes ofMycoplasma hyopneumoniae . Current Microbiology 24, 189–192 (1992). https://doi.org/10.1007/BF01579280
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DOI: https://doi.org/10.1007/BF01579280