Abstract
Ala-1 (activated lymphocyte antigen-1) is a murine alloantigen expressed on mitogen-stimulated peripheral T and B cells. C3H/An mice were immunized with PHA-stimulated C58 lymphocytes; reciprocal immunizations were also performed. After multiple absorptions to remove unwanted antibody specificities, the antiserum did not lyse thymocytes, lymph node, or spleen cells, but killed more than 90% of Con A-stimulated T cells and more than 90% of LPS-stimulated B cells in cytotoxicity tests. Quantitative absorption studies confirmed that thymocytes are Ala-1−, but revealed the presence of some Ala-1 antigen in normal lymph node and spleen populations. The strain distribution of Ala-1 was determined for 23 inbred strains. The reactions of the two reciprocal antisera (C3H anti-C58, and C58 anti-C3H) were mutually exclusive on all strains tested, indicating that the antisera probably recognize antithetical forms of Ala-1. Since thymocytes cultured with Con A do not express Ala-1, whereas peripheral mitogen-stimulated cells do, we propose that Ala-1 is a differentiation antigen, the expression of which is restricted to the late stages of development of T and B cells.
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Abbreviations
- Con A:
-
concanavalin A
- PHA:
-
phytohemagglutinin
- LPS:
-
lipopolysaccharide
- C:
-
complement
- BSA:
-
bovine serum albumin
- B6:
-
C57BL/6 mice
- FBS:
-
fetal bovine serum
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Feeney, A.J., Hämmerling, U. Ala-1: A murine alloantigen of activated lymphocytes. Immunogenetics 3, 369–379 (1976). https://doi.org/10.1007/BF01576968
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DOI: https://doi.org/10.1007/BF01576968